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• Programmable and resilient metamaterials with anisotropic and non-linear mechanical responses composed exclusively of stiff constituents.
  Recently, significant progress has been made in the field of flexible bulk metamaterials composed of soft and elastic materials, unlocking the potential for achieving programmable non-linear mechanical responses, such as shape morphing, energy absorption, and …
  pubmed Yao H, Zhao X, Shi K, Sun W 等 2024 Sep 30 置信度 0.82
• Trehalose along with ABA promotes the salt tolerance of Avicennia marina by regulating Na(+) transport.
  Mangroves grow in tropical/subtropical intertidal habitats with extremely high salt tolerance. Trehalose and trehalose-6-phosphate (T6P) have an alleviating function against abiotic stress. However, the roles of trehalose in the salt tolerance of salt-secretin…
  pubmed Song LY, Xu CQ, Zhang LD, Li J 等 2024 Sep 置信度 0.82
• Fine and combinatorial regulation of key metabolic pathway for enhanced β-alanine biosynthesis with non-inducible Escherichia coli.
  β-Alanine is the only β-amino acid in nature and one of the most important three-carbon chemicals. This work was aimed to construct a non-inducible β-alanine producer with enhanced metabolic flux towards β-alanine biosynthesis in Escher…
  pubmed Zhou HY, Ding WQ, Zhang X, Zhang HY 等 2024 Oct 置信度 0.82
• Comparing the antioxidation and bioavailability of polysaccharides from extruded and unextruded Baijiu vinasses via in vitro digestion and fecal fermentation.
  Extrusion has been proven to be a novel approach for modifying the physicochemical characteristic of Baijiu vinasses (BV) to extract polysaccharides, contributing to the sustainable development of brewing industry. However, the comparison of the bioactivity an…
  pubmed Liu Y, Li X, Qin H, Huang M 等 2024 Sep 置信度 0.82
• Harnessing the potential of microalgae for the production of monoclonal antibodies and other recombinant proteins.
  Monoclonal antibodies (mAbs) have become indispensable tools in various fields, from research to therapeutics, diagnostics, and industries. However, their production, primarily in mammalian cell culture systems, is cost-intensive and resource-demanding. Microa…
  pubmed Rajput BK, Ikram SF, Tripathi BN 2024 Nov 置信度 0.82
• Protein rational design and modification of erythrose reductase for the improvement of erythritol production in Yarrowia lipolytica.
  Erythritol is a natural non-caloric sweetener, which is produced by fermentation and extensively applied in food, medicine and chemical industries. The final step of the erythritol synthesis pathway is involved in erythritol reductase, whose activity and NADPH…
  pubmed Huang L, Wang W, Wang K, Li Y 等 2024 Oct 置信度 0.82
• Transcriptome analysis reveals hepatic disordered lipid metabolism, lipotoxic injury, and abnormal development in IUGR sheep fetuses due to maternal undernutrition during late pregnancy.
  Although lipid metabolism in fetal livers under intrauterine growth restriction (IUGR) conditions has been widely studied, the implications of maternal undernutrition on fetal hepatic lipid metabolism, lipotoxic injury, and abnormal development remain largely …
  pubmed Zi Y, Qin Y, Ma C, Qiao Y 等 2024 Sep 15 置信度 0.82
• Antibody glycan quality predicted from CHO cell culture media markers and machine learning.
  N-glycosylation can have a profound effect on the quality of mAb therapeutics. In biomanufacturing, one of the ways to influence N-glycosylation patterns is by altering the media used to grow mAb cell expression systems. Here, we explore the potential of machi…
  pubmed Lakshmanan M, Chia S, Pang KT, Sim LC 等 2024 Dec 置信度 0.82
• Potential nutritional and functional matters in yeast culture prepared by soybean meal fermentation.
  Yeast culture (YC) is a product fermented on a specific medium, which is a type of postbiotic of anaerobic solid-state fermentation. Although YC has positive effects on the animal growth and health, it contains a variety of beneficial metabolites as dark matte…
  pubmed Cao Y, Xu M, Chen Q, Wu D 等 2024 Nov 置信度 0.82
• Antibiotic synergistic effect surge bioenergy potential and pathogen resistance of Chlorella variabilis biofilm.
  Tetracycline (TC) and ciprofloxacin (CF) induce a synergistic effect that alters the biochemical composition, leading to a decrease in the growth and photosynthetic efficiency of microalgae. But the current study provides a novel insight into stress-inducing t…
  pubmed Ugya AY, Hasan DB, Ari HA, Sheng Y 等 2024 Oct 15 置信度 0.82
• Vibrational imaging of metabolites for improved microbial cell strains.
  Biomanufacturing utilizes modified microbial systems to sustainably produce commercially important biomolecules for use in agricultural, energy, food, material, and pharmaceutical industries. However, technological challenges related to non-destructive and hig…
  pubmed Hanninen A 2024 Jun 置信度 0.82
• Co-immobilization of whole cells and enzymes by covalent organic framework for biocatalysis process intensification.
  Co-immobilization of cells and enzymes is often essential for the cascade biocatalytic processes of industrial-scale feasibility but remains a vast challenge. Herein, we create a facile co-immobilization platform integrating enzymes and cells in covalent organ…
  pubmed Zheng D, Zheng Y, Tan J, Zhang Z 等 2024 Jun 29 置信度 0.82
• Simultaneously Enhanced Catalytic Activity and Thermostability of a Baeyer-Villiger Monooxygenase from Oceanicola granulosus by Reshaping the Binding Pocket.
  Enzymatic oxygenation of various cyclic ketones into lactones via Baeyer-Villiger monooxygenases (BVMOs) could provide a promising route for synthesizing fragrances and pharmaceutical ingredients. However, unsatisfactory catalytic activity and thermostability …
  pubmed Du Y, Lv X, Feng C, Ma Y 等 2024 Jul 17 置信度 0.82
• Gastric Cancer Assembloids Derived from Patient-Derived Xenografts: A Preclinical Model for Therapeutic Drug Screening.
  The construction of reliable preclinical models is crucial for understanding the molecular mechanisms involved in gastric cancer and for advancing precision medicine. Currently, existing in vitro tumor models often do not accurately replicate the human gastric…
  pubmed Xu X, Gao Y, Dai J, Wang Q 等 2024 Sep 置信度 0.82
• Enzymatic Routes for Chiral Amine Synthesis: Protein Engineering and Process Optimization.
  Chiral amines are essential motifs in pharmaceuticals, agrochemicals, and specialty chemicals. While traditional chemical routes to chiral amines often lack stereoselectivity and require harsh conditions, biocatalytic methods using engineered enzymes can offer…
  pubmed Vikhrankar SS, Satbhai S, Kulkarni P, Ranbhor R 等 2024 置信度 0.82
• Harnessing the potential of hydrogels for advanced therapeutic applications: current achievements and future directions.
  The applications of hydrogels have expanded significantly due to their versatile, highly tunable properties and breakthroughs in biomaterial technologies. In this review, we cover the major achievements and the potential of hydrogels in therapeutic application…
  pubmed Lu P, Ruan D, Huang M, Tian M 等 2024 Jul 1 置信度 0.82
• Biosensors for the detection of chorismate and cis,cis-muconic acid in Corynebacterium glutamicum.
  Corynebacterium glutamicum ATCC 13032 is a promising microbial chassis for industrial production of valuable compounds, including aromatic amino acids derived from the shikimate pathway. In this work, we developed two whole-cell, transcription factor based flu…
  pubmed Velasquez-Guzman JC, Huttanus HM, Morales DP, Werner TS 等 2024 Jan 9 置信度 0.82
• Extracellular vesicle biogenesis of three-dimensional human pluripotent stem cells in a novel Vertical-Wheel bioreactor.
  Extracellular vesicles (EVs) secreted by human-induced pluripotent stem cells (hiPSCs) have great potential as cell-free therapies in various diseases, including prevention of blood-brain barrier senescence and stroke. However, there are still challenges in pr…
  pubmed Muok L, Sun L, Esmonde C, Worden H 等 2024 Jan 置信度 0.82
• Establishing a Cell-Free Glycoprotein Synthesis System for Enzymatic N-GlcNAcylation.
  N -linked glycosylation plays a key role in the efficacy of many therapeutic proteins. One limitation to the bacterial glycoengineering of human N -linked glycans is the difficulty of installing a single N -acetylglucosamine (GlcNAc), the reducing end sugar of…
  pubmed DeWinter MA, Wong DA, Fernandez R, Kightlinger W 等 2024 Jul 19 置信度 0.82
• The G-Protein-Coupled Estrogen Receptor Selective Agonist G-1 Attenuates Cell Viability and Migration in High-Grade Serous Ovarian Cancer Cell Lines.
  The G-protein-coupled estrogen receptor (GPER; G-protein-coupled estrogen receptor 30, also known as GPR30) is a novel estrogen receptor and has emerged as a promising target for ovarian cancer. GPER, a seven-transmembrane receptor, suppresses cellular viabili…
  pubmed Hanafi D, Onyenwoke RU, Kimbro KS 2024 Jun 13 置信度 0.82
• Integration Approaches to Model Bioreactor Hydrodynamics and Cellular Kinetics for Advancing Bioprocess Optimisation.
  Large-scale bioprocesses are increasing globally to cater to the larger market demands for biological products. As fermenter volumes increase, the efficiency of mixing decreases, and environmental gradients become more pronounced compared to smaller scales. Co…
  pubmed Singh VK, Jiménez Del Val I, Glassey J, Kavousi F 2024 May 27 置信度 0.82
• CRISPR Deletion of miR-27 Impacts Recombinant Protein Production in CHO Cells.
  MicroRNAs represent an interesting group of regulatory molecules with the unique ability of a single miRNA able to regulate the expression of potentially hundreds of target genes. In that regard, their utility has been demonstrated as a strategy to improve the…
  pubmed Kellner K, Lao NT, Barron N 2024 置信度 0.82
• Decoding the Qu-aroma of medium-temperature Daqu starter by volatilomics, aroma recombination, omission studies and sensory analysis.
  Qu-aroma is of great significance for evaluation the quality of Daqu starter. This study aimed to decode the Qu-aroma of medium-temperature Daqu (MT-Daqu) via "top-down" and "bottom-up" approaches. Firstly, 52 aroma descriptors were defined to describe the MT-…
  pubmed Yang SB, Fu JJ, He JH, Zhang XJ 等 2024 Nov 1 置信度 0.82
• The Performance and Evolutionary Mechanism of Ganoderma lucidum in Enhancing Selenite Tolerance and Bioaccumulation.
  Selenium (Se) pollution poses serious threats to terrestrial ecosystems. Mushrooms are important sources of Se with the potential for bioremediation. Pre-eminent Se resources must possess the ability to tolerate high levels of Se. To obtain Se-accumulating fun…
  pubmed Xu M, Meng Q, Zhu S, Yu R 等 2024 Jun 8 置信度 0.82
• Bridging Nature and Engineering: Protein-Derived Materials for Bio-Inspired Applications.
  The sophisticated, elegant protein-polymers designed by nature can serve as inspiration to redesign and biomanufacture protein-based materials using synthetic biology. Historically, petro-based polymeric materials have dominated industrial activities, conseque…
  pubmed Nawaz T, Gu L, Gibbons J, Hu Z 等 2024 Jun 20 置信度 0.82
• Lipid-Encapsulated Engineered Bacterial Living Materials Inhibit Cyclooxygenase II to Enhance Doxorubicin Toxicity.
  Recently, there has been increasing interest in the use of bacteria for cancer therapy due to their ability to selectively target tumor sites and inhibit tumor growth. However, the complexity of the interaction between bacteria and tumor cells evokes unpredict…
  pubmed Jiang N, Ding W, Zhu X, Chen J 等 2024 置信度 0.82
• Engineering a Robust UDP-Glucose Pyrophosphorylase for Enhanced Biocatalytic Synthesis via ProteinMPNN and Ancestral Sequence Reconstruction.
  UDP-glucose is a key metabolite in carbohydrate metabolism and plays a vital role in glycosyl transfer reactions. Its significance spans across the food and agricultural industries. This study focuses on UDP-glucose synthesis via multienzyme catalysis using de…
  pubmed Li Z, Lou M, Sun C, Li Z 2024 Jul 10 置信度 0.82
• Recent advances in lycopene and germacrene a biosynthesis and their role as antineoplastic drugs.
  Sesquiterpenes and tetraterpenes are classes of plant-derived natural products with antineoplastic effects. While plant extraction of the sesquiterpene, germacrene A, and the tetraterpene, lycopene suffers supply chain deficits and poor yields, chemical synthe…
  pubmed Fordjour E, Liu CL, Yang Y, Bai Z 2024 Jun 25 置信度 0.82
• [Graduate teaching reform on synthetic biology featuring the discipline of fermentation engineering].
  Industrial biotechnology is regarded as the most promising technology for sustainable industrial development. The advancement of synthetic biology creates new opportunities and infinite possibilities for the progress of industrial biotechnology. Fermentation e…
  pubmed Xu G, Yu S, Zhou J 2024 Jun 25 置信度 0.82
• Design and development of 3D printed shape memory triphasic polymer-ceramic bioactive scaffolds for bone tissue engineering.
  Scaffolds for bone tissue engineering require considerable mechanical strength to repair damaged bone defects. In this study, we designed and developed mechanically competent composite shape memory triphasic bone scaffolds using fused filament fabrication (FFF…
  pubmed Ansari MAA, Makwana P, Dhimmar B, Vasita R 等 2024 Jul 17 置信度 0.82
• GC/HRMS Analysis of E-Liquids Complements In Vivo Modeling Methods and can Help to Predict Toxicity.
  Tobacco smoking is a major risk factor for disease development, with the user inhaling various chemicals known to be toxic. However, many of these chemicals are absent before tobacco is "burned". Similar, detailed data have only more recently being reported fo…
  pubmed Walker-Franklin I, Onyenwoke RU, Leung T, Huang X 等 2024 Jun 18 置信度 0.82
• Gongronella sp. w5 hydrolyzes plant sucrose and releases fructose to recruit phosphate-solubilizing bacteria to provide plants with phosphorus.
  The mechanisms of how plant-beneficial rhizospheric fungi interact with the soil microbial community to promote plant growth by facilitating their phosphorus acquisition are poorly understood. This work supported that a Mucoromycotina fungus, Gongronella sp. w…
  pubmed Wang X, Fang J, Li L, Li X 等 2024 Jul 24 置信度 0.82
• Polyextremophile engineering: a review of organisms that push the limits of life.
  Nature exhibits an enormous diversity of organisms that thrive in extreme environments. From snow algae that reproduce at sub-zero temperatures to radiotrophic fungi that thrive in nuclear radiation at Chernobyl, extreme organisms raise many questions about th…
  pubmed Caro-Astorga J, Meyerowitz JT, Stork DA, Nattermann U 等 2024 置信度 0.82
• A long-term growth stable Halomonas sp. deleted with multiple transposases guided by its metabolic network model Halo-ecGEM.
  Microbial instability is a common problem during bio-production based on microbial hosts. Halomonas bluephagenesis has been developed as a chassis for next generation industrial biotechnology (NGIB) under open and unsterile conditions. However, the hidden geno…
  pubmed Zhang L, Ye JW, Li G, Park H 等 2024 Jul 置信度 0.82
• Engineering carbon source division of labor for efficient α-carotene production in Corynebacterium glutamicum.
  Effective utilization of glucose, xylose, and acetate, common carbon sources in lignocellulose hydrolysate, can boost biomanufacturing economics. However, carbon leaks into biomass biosynthesis pathways instead of the intended target product remain to be optim…
  pubmed Li K, Li C, Liu CG, Zhao XQ 等 2024 Jul 置信度 0.82
• Advances in aldo-keto reductases immobilization for biocatalytic synthesis of chiral alcohols.
  Chiral alcohols are essential building blocks of numerous pharmaceuticals and fine chemicals. Aldo-keto reductases (AKRs) constitute a superfamily of oxidoreductases that catalyze the reduction of aldehydes and ketones to their corresponding alcohols using NAD…
  pubmed Zhang W, Shao ZQ, Wang ZX, Ye YF 等 2024 Aug 置信度 0.82
• Two Zn(2)Cys(6)-type transcription factors respond to aromatic compounds and regulate the expression of laccases in the white-rot fungus Trametes hirsuta.
  White-rot fungi differentially express laccases when they encounter aromatic compounds. However, the underlying mechanisms are still being explored. Here, proteomics analysis revealed that in addition to increased laccase activity, proteins involved in sphingo…
  pubmed Wang C, Zhang X, Wu K, Liu S 等 2024 Jul 24 置信度 0.82
• Construction of a Novel Vanillin-Induced Autoregulating Bidirectional Transport System in a Vanillin-Producing E. coli Cell Factory.
  Vanillin is one of the world's most extensively used flavoring agents with high application value. However, the yield of vanillin biosynthesis remains limited due to the low efficiency of substrate uptake and the inhibitory effect on cell growth caused by vani…
  pubmed Li Z, Sun L, Wang Y, Liu B 等 2024 Jul 3 置信度 0.82
• RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β and WFS1 knockdown on fed-batch production of IgG(1).
  Secretion levels required of industrial Chinese hamster ovary (CHO) cell lines can challenge endoplasmic reticulum (ER) homeostasis, and ER stress caused by accumulation of misfolded proteins can be a bottleneck in biomanufacturing. The unfolded protein respon…
  pubmed Rives D, Peak C, Blenner MA 2024 Jun 19 置信度 0.82
• Intein-mediated temperature control for complete biosynthesis of sanguinarine and its halogenated derivatives in yeast.
  While sanguinarine has gained recognition for antimicrobial and antineoplastic activities, its complex conjugated structure and low abundance in plants impede broad applications. Here, we demonstrate the complete biosynthesis of sanguinarine and halogenated de…
  pubmed Gou Y, Li D, Zhao M, Li M 等 2024 Jun 19 置信度 0.82
• From waste to protein: a new strategy of converting composted distilled grain wastes into animal feed.
  Distilled grain waste (DGW) is rich in nutrients and can be a potential resource as animal feed. However, DGW contains as much as 14% lignin, dramatically reducing the feeding value. White-rot fungi such as Pleurotus ostreatus could preferentially degrade lign…
  pubmed Yu L, An Z, Xie D, Yin D 等 2024 置信度 0.82
• Biochemical Characterization of a Marine Pseudoalteromonas citrea-Derived Fatty Acyl-AMP Ligase That Exhibits N-Acyl Amino Acid Synthetic Activity.
  Activation of fatty acids as acyl-adenylates by fatty acid-AMP ligase (FAAL) is a well-established process contributing to the formation of various functional natural products. Enzymatic characterization of FAALs is pivotal for unraveling both the catalytic me…
  pubmed Li K, Deng F, Wang Y, Wang F 2024 Dec 置信度 0.82
• Lipase-catalyzed interesterification of Sunite sheep tail fat and flaxseed oil provides a fat having a unique fatty acid content and favorable physicochemical and nutritional properties.
  The aim of this study is to combine flaxseed oil (FO), rich in α-linolenic acid (ALA), with Sunite sheep tail fat (STF) through a lipase-catalyzed transesterification reaction, in order to produce an edible oil with a fatty acid ratio suitable for human …
  pubmed Baoyindugurong JH, Hou JL, Ren YN, Li YW 等 2024 Jun 置信度 0.82
• CRISPR/Cas9-Based Genome Editing for Protein Expression and Secretion in Kluyveromyces lactis.
  The budding yeast Kluyveromyces lactis has emerged as a promising microbial chassis in industrial biotechnology. However, a lack of efficient molecular genetic manipulation tools and strategies has hindered the development of K. lactis as a biomanufacturing pl…
  pubmed Liao L, Shen X, Shen Z, Du G 等 2024 Jul 19 置信度 0.82
• Combinatorial metabolic engineering of Escherichia coli for de novo production of structurally defined and homogeneous Amino oligosaccharides.
  Amino oligosaccharides (AOs) possess various biological activities and are valuable in the pharmaceutical, food industries, and agriculture. However, the industrial manufacturing of AOs has not been realized yet, despite reports on physical, chemical, and biol…
  pubmed Shi J, Deng C, Zhang C, Quan S 等 2024 Dec 置信度 0.82
• Leveraging Senescent Cancer Cell Membrane to Potentiate Cancer Immunotherapy Through Biomimetic Nanovaccine.
  Senescent cancer cells are endowed with high immunogenic potential that has been leveraged to elicit antitumor immunity and potentially complement anticancer therapies. However, the efficacy of live senescent cancer cell-based vaccination is limited by interfe…
  pubmed Yang C, Chen Y, Liu J, Zhang W 等 2024 Aug 置信度 0.82
• Construction and Function of Thiolate-Bridged Diiron N(x)H(y) Nitrogenase Model Complexes.
  ConspectusBiological nitrogen fixation mediated by nitrogenases has garnered significant research interest due to its critical importance to the development of efficient catalysts for mild ammonia synthesis. Although the active center of the most studied FeMo-…
  pubmed Yang D, Wang B, Qu J 2024 Jul 2 置信度 0.82
• Thermostable in vitro transcription-translation compatible with microfluidic droplets.
  In vitro expression involves the utilization of the cellular transcription and translation machinery in an acellular context to produce one or more proteins of interest and has found widespread application in synthetic biology and in pharmaceutical biomanufact…
  pubmed Ribeiro ALJL, Pérez-Arnaiz P, Sánchez-Costa M, Pérez L 等 2024 Jun 10 置信度 0.82
• Molecular biomarkers identification and applications in CHO bioprocessing.
  Biomarkers are valuable tools in clinical research where they allow to predict susceptibility to diseases, or response to specific treatments. Likewise, biomarkers can be extremely useful in the biomanufacturing of therapeutic proteins. Indeed, constraints suc…
  pubmed Desmurget C, Perilleux A, Souquet J, Borth N 等 2024 Sep 10 置信度 0.82
• A novel thermo-responsive phospholipase A(1) with high selectivity and efficiency in enzymatic oil degumming.
  The limited availability of phospholipase A 1 (PLA 1 ) has posed significant challenges in enzymatic degumming. In this study, a novel PLA1 (UM2) was introduced to address this limitation, which had a unique thermo-responsive ability to switch phospholipase an…
  pubmed Li Z, Zhou H, Liu X, Wang W 等 2024 Oct 30 置信度 0.82
• Advances and opportunities in process analytical technologies for viral vector manufacturing.
  Viral vectors are an emerging, exciting class of biologics whose application in vaccines, oncology, and gene therapy has grown exponentially in recent years. Following first regulatory approval, this class of therapeutics has been vigorously pursued to treat m…
  pubmed Sripada SA, Hosseini M, Ramesh S, Wang J 等 2024 Sep 置信度 0.82
• Glutamine-derived peptides: Current progress and future directions.
  Glutamine, the most abundant amino acid in the body, plays a critical role in preserving immune function, nitrogen balance, intestinal integrity, and resistance to infection. However, its limited solubility and instability present challenges for its use a func…
  pubmed Yu T, Hu T, Na K, Zhang L 等 2024 Jul 置信度 0.82
• Mechanism-based and data-driven modeling in cell-free synthetic biology.
  Cell-free systems have emerged as a versatile platform in synthetic biology, finding applications in various areas such as prototyping synthetic circuits, biosensor development, and biomanufacturing. To streamline the prototyping process, cell-free systems oft…
  pubmed Yurchenko A, Özkul G, van Riel NAW, van Hest JCM 等 2024 Jun 20 置信度 0.82
• Engineering a carbon source-responsive promoter for improved biosynthesis in the non-conventional yeast Kluyveromyces marxianus.
  Many desired biobased chemicals exhibit a range of toxicity to microbial cell factories, making industry-level biomanufacturing more challenging. Separating microbial growth and production phases is known to be beneficial for improving production of toxic prod…
  pubmed Bassett S, Da Silva NA, Shane Bassett, Nancy A. Da Silva 2024 Jun 置信度 0.82
• Unleashing plant synthetic capacity: navigating regulatory mechanisms for enhanced bioproduction and secondary metabolite discovery.
  Plant natural products (PNPs) hold significant pharmaceutical importance. The sessile nature of plants has led to the evolution of chemical defense mechanisms over millions of years to combat environmental challenges, making it a crucial and essential defense …
  pubmed Hu R, Teng X, Li Y 2024 Aug 置信度 0.82
• Interfacing data science with cell therapy manufacturing: where we are and where we need to be.
  Although several cell-based therapies have received FDA approval, and others are showing promising results, scalable, and quality-driven reproducible manufacturing of therapeutic cells at a lower cost remains challenging. Challenges include starting material a…
  pubmed Wang B, Chen RQ, Li J, Roy K 2024 Sep 置信度 0.82
• Propelling Minimally Invasive Tissue Regeneration With Next-Era Injectable Pre-Formed Scaffolds.
  The growing aging population, with its associated chronic diseases, underscores the urgency for effective tissue regeneration strategies. Biomaterials play a pivotal role in the realm of tissue reconstruction and regeneration, with a distinct shift toward mini…
  pubmed Liao J, Timoshenko AB, Cordova DJ, Astudillo Potes MD 等 2024 Aug 置信度 0.82
• Design of NAMPTs with Superior Activity by Dual-Channel Protein Engineering Strategy.
  The nicotinamide phosphoribosyltransferase (NAMPT)-catalyzed substitution reaction plays a pivotal role in the biosynthesis of nucleotide compounds. However, industrial applications are hindered by the low activity of NAMPTs. In this study, a novel dual-channe…
  pubmed Peng F, Shen Q, Zou LP, Cheng F 等 2024 Jun 6 置信度 0.82
• Characterization of DmToll and DmToll7 homologue in Litopenaeus vannamei based on structure analysis.
  Toll-like receptors (TLRs) are a family of pattern recognition receptors (PRRs) that recognize invading pathogens and activate downstream signaling pathways. The number of 10 Tolls is found in Litopenaeus vannamei but have not yet been identified as the corres…
  pubmed Shi C, Tang Z, Jin Z, Huang S 等 2024 Sep 置信度 0.82
• SpheroMold: modernizing the hanging drop method for spheroid culture.
  The hanging drop method is a cost-effective approach for 3D spheroid culture. However, obtaining numerous spheroids in a limited area becomes challenging due to the risk of droplet coalescence, primarly during Petri dish handling. In this study, we describe a …
  pubmed Guimaraes APP, Calori IR, Bi H, Tedesco AC 2024 置信度 0.82
• Microbial Degradation of Tetracycline Antibiotics: Mechanisms and Environmental Implications.
  The escalating global consumption of tetracyclines (TCs) as broad-spectrum antibiotics necessitates innovative approaches to mitigate their pervasive environmental persistence and associated risks. While initiatives such as China's antimicrobial reduction effo…
  pubmed Li Q, Zheng Y, Guo L, Xiao Y 等 2024 Jun 4 置信度 0.82
• A cost-saving, safe, and highly efficient natural mediator for laccase application on aflatoxin detoxification.
  Laccase mediators possess advantage of oxidizing substrates with high redox potentials, such as aflatoxin B 1 (AFB 1 ). High costs of chemically synthesized mediators limit laccase industrial application. In this study, thin stillage extract (TSE), a byproduct…
  pubmed Liu S, Zhou Y, Feng Y, Peng Q 等 2024 Oct 15 置信度 0.82
• Multi-cell-line learning for the data-driven construction of mechanistic metabolic models.
  Mammalian cells are commonly used as hosts in cell culture for biologics production in the pharmaceutical industry. Structured mechanistic models of metabolism have been used to capture complex cellular mechanisms that contribute to varying metabolic shifts in…
  pubmed Lu YA, McCann MG, Hu WS, Zhang Q 2024 Sep 置信度 0.82
• Pooled CRISPR Interference Screening Identifies Crucial Transcription Factors in Gas-Fermenting Clostridium ljungdahlii.
  Gas-fermenting Clostridium species hold tremendous promise for one-carbon biomanufacturing. To unlock their full potential, it is crucial to unravel and optimize the intricate regulatory networks that govern these organisms; however, this aspect is currently u…
  pubmed Zhang H, Feng H, Xing XH, Jiang W 等 2024 Jun 21 置信度 0.82
• Toward biomanufacturing of next-generation bacterial nanocellulose (BNC)-based materials with tailored properties: A review on genetic engineering approaches.
  Bacterial nanocellulose (BNC) is a biopolymer that is drawing significant attention for a wide range of applications thanks to its unique structure and excellent properties, such as high purity, mechanical strength, high water holding capacity and biocompatibi…
  pubmed Núñez D, Oyarzún P, González S, Martínez I 2024 Sep 置信度 0.82
• Preparation of medium- and long-chain triacylglycerols rich in n-3 polyunsaturated fatty acids by bio-imprinted lipase-catalyzed interesterification.
  Medium and long-chain triacylglycerol (MLCT) rich in n-3 polyunsaturated fatty acids (PUFAs) were obtained in three-hour interesterification of fish oil with medium-chain triacylglycerol (MCTs), using lipase bio-imprinted with surfactant as a catalyst. Initial…
  pubmed Zou X, Khan I, Wang Y, Hussain M 等 2024 Oct 15 置信度 0.82
• Nanomaterials-Based Hybrid Bioink Platforms in Advancing 3D Bioprinting Technologies for Regenerative Medicine.
  3D bioprinting is recognized as the ultimate additive biomanufacturing technology in tissue engineering and regeneration, augmented with intelligent bioinks and bioprinters to construct tissues or organs, thereby eliminating the stipulation for artificial orga…
  pubmed Chandra DK, Reis RL, Kundu SC, Kumar A 等 2024 Jul 8 置信度 0.82
• Development of growth selection system and pocket engineering of d-amino acid oxidase to enhance selective deamination activity toward d-phosphinothricin.
  D-amino acid oxidase (DAAO)-catalyzed selective oxidative deamination is a very promising process for synthesizing l-amino acids including l-phosphinothricin (l-PPT, a high-efficiency and broad-spectrum herbicide). However, the wild-type DAAO's low activity to…
  pubmed Cheng F, Sun KX, Gong XX, Peng W 等 2024 Sep 置信度 0.82
• A bioprinted sea-and-island multicellular model for dissecting human pancreatic tumor-stroma reciprocity and adaptive metabolism.
  Pancreatic ductal adenocarcinoma (PDAC) presents a formidable clinical challenge due to its intricate microenvironment characterized by desmoplasia and complex tumor-stroma interactions. Conventional models hinder studying cellular crosstalk for therapeutic de…
  pubmed Li M, Freeman S, Franco-Barraza J, Cai KQ 等 2024 Oct 置信度 0.82
• Surface-displayed silicatein-α enzyme in bioengineered E. coli enables biocementation and silica mineralization.
  Biocementation is an exciting biomanufacturing alternative to common cement, which is a significant contributor of CO 2 greenhouse gas production. In nature biocementation processes are usually modulated via ureolytic microbes, such as Sporosarcina pasteurii, …
  pubmed Vigil TN, Schwendeman NK, Grogger MLM, Morrison VL 等 2024 置信度 0.82
• Enhancing the Thermal Stability and Enzyme Activity of Ketopantoate Hydroxymethyltransferase through Interface Modification Engineering.
  Ketopantoate hydroxymethyltransferase (KPHMT) plays a pivotal role in d-pantothenic acid biosynthesis. Most KPHMTs are homodecamers with low thermal stability, posing challenges for protein engineering and limiting output enhancement. Previously, a high-enzyme…
  pubmed Cai X, Shi X, Wang JY, Hu CH 等 2024 Jun 12 置信度 0.82
• Reinforcement learning-guided control strategies for CAR T-cell activation and expansion.
  Reinforcement learning (RL), a subset of machine learning (ML), could optimize and control biomanufacturing processes, such as improved production of therapeutic cells. Here, the process of CAR T-cell activation by antigen-presenting beads and their subsequent…
  pubmed Ferdous S, Shihab IF, Chowdhury R, Reuel NF 2024 Sep 置信度 0.82
• LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.
  Temperature is an important control factor for biologics biomanufacturing in precision fermentation. Here, we explored a highly responsive low temperature-inducible genetic system (LowTempGAL) in the model yeast Saccharomyces cerevisiae. Two temperature biosen…
  pubmed Lu Z, Shen Q, Bandari NC, Evans S 等 2024 Jul 8 置信度 0.82
• Removal of empty capsids from high-dose adeno-associated virus 9 gene therapies.
  Recombinant adeno-associated virus, serotype 9 (rAAV9) has shown promise as a gene therapy vector for muscle and central nervous diseases. High-dose requirements of these therapies present critical safety considerations and biomanufacturing challenges. Notably…
  pubmed Kish WS, Lightholder J, Zeković T, Berrill A 等 2024 Aug 置信度 0.82
• Multiangle Light Scattering as a Lentivirus Purification Process Analytical Technology.
  The limited biomolecular and functional stability of lentiviral vectors (LVVs) for cell therapy poses the need for analytical tools that can monitor their titers and activity throughout the various steps of expression and purification. In this study, we descri…
  pubmed Sripada SA, Barbieri E, Shastry S, Wuestenhagen E 等 2024 Jun 11 置信度 0.82
• Structural Insights of a cis-Epoxysuccinate Hydrolase Facilitate the Development of Robust Biocatalysts for the Production of l-(+)-Tartrate.
  l-(+)-Tartaric acid plays important roles in various industries, including pharmaceuticals, foods, and chemicals. cis -Epoxysuccinate hydrolases (CESHs) are crucial for converting cis -epoxysuccinate to l-(+)-tartrate in the industrial production process. Ther…
  pubmed Han Y, Luo Y, Ma BD, Li J 等 2024 Jun 18 置信度 0.82
• Use of acetate as substrate for sustainable production of homoserine and threonine by Escherichia coli W3110: A modular metabolic engineering approach.
  Acetate, a promising yet underutilized carbon source for biological production, was explored for the efficient production of homoserine and threonine in Escherichia coli W. A modular metabolic engineering approach revealed the crucial roles of both acetate ass…
  pubmed Vo TM, Park JY, Kim D, Park S 等 2024 Jul 置信度 0.82
• Status and future developments for downstream processing of biological products: Perspectives from the Recovery XIX yield roundtable discussions.
  Governments and biopharmaceutical organizations aggressively leveraged expeditious communication capabilities, decision models, and global strategies to make a COVID-19 vaccine happen within a period of 12 months. This was an unusual effort and cannot be trans…
  pubmed Jungbauer A, Ferreira G, Butler M, D'Costa S 等 2024 Aug 置信度 0.82
• Optimization of Culture Media and Feeding Strategy for High Titer Production of an Adenoviral Vector in HEK 293 Fed-Batch Culture.
  Adenoviruses are efficient and safe vectors for delivering target antigens and adenovirus-based vaccines have been used against a wide variety of pathogens, including tuberculosis and COVID-19. Cost-effective and scalable biomanufacturing processes are critica…
  pubmed Shen CF, Rodenbrock A, Lanthier S, Burney E 等 2024 May 10 置信度 0.82
• B355252 Suppresses LPS-Induced Neuroinflammation in the Mouse Brain.
  B355252 is a small molecular compound known for potentiating neural growth factor and protecting against neuronal cell death induced by glutamate in vitro and cerebral ischemia in vivo. However, its other biological functions remain unclear. This study aims to…
  pubmed He Q, Qi Q, Ibeanu GC, Li PA 2024 May 7 置信度 0.82
• Engineering of Saccharomyces cerevisiae for enhanced metabolic robustness and L-lactic acid production from lignocellulosic biomass.
  Metabolic engineering for high productivity and increased robustness is needed to enable sustainable biomanufacturing of lactic acid from lignocellulosic biomass. Lactic acid is an important commodity chemical used for instance as a monomer for production of p…
  pubmed Choi B, Tafur Rangel A, Kerkhoven EJ, Nygård Y 等 2024 Jul 置信度 0.82
• Advances and perspectives in genetic expression and operation for the oleaginous yeast Yarrowia lipolytica.
  The utilization of industrial biomanufacturing has emerged as a viable and sustainable alternative to fossil-based resources for producing functional chemicals. Moreover, advancements in synthetic biology have created new opportunities for the development of i…
  pubmed Hu M, Ge J, Jiang Y, Sun X 等 2024 Dec 置信度 0.82
• [3D tumor spheroids promote activation, expansion, and anti-tumor effects of tumor-infiltrating lymphocytes in vitro].
  The adoptive immunotherapy mediated by tumor-infiltrating lymphocytes (TILs) has shown definite efficacy against various solid tumors. However, the inefficiency of the conventional method based on in vitro expansion of TILs fails to achieve the cell count and …
  pubmed Xu X, Zhang Y, Wang Z, Zhang Q 等 2024 May 25 置信度 0.82
• Highly efficient production of 2-phenylethanol by wild-type Saccharomyces bayanus strain.
  2-Phenylethanol (2-PE) is a highly valuable aromatic alcohol utilized in fragrance, cosmetics and food industries. Due to the toxic by-products from chemical synthesis and the low productivity of the extraction method, bioproduction of 2-PE by yeast is conside…
  pubmed Zhao Y, Li S, Shu Q, Yang X 等 2024 Jul 置信度 0.82
• Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana.
  Cell-surface receptors form the front line of plant immunity. The leucine-rich repeat (LRR)-receptor-like kinases SOBIR1 and BAK1 are required for the functionality of the tomato LRR-receptor-like protein Cf-4, which detects the secreted effector Avr4 of the p…
  pubmed Huang WRH, Braam C, Kretschmer C, Villanueva SL 等 2024 May 21 置信度 0.82
• Exploring the molecular mechanisms of Lrp/AsnC-type transcription regulator DecR, an L-cysteine-responsive feast/famine regulatory protein.
  The Lrp/AsnC family of transcriptional regulators is commonly found in prokaryotes and is associated with the regulation of amino acid metabolism. However, it remains unclear how the L-cysteine-responsive Lrp/AsnC family regulator perceives and responds to L-c…
  pubmed Zhou Z, Li Z, Li Z 2024 Jun 置信度 0.82
• Validation of a next generation sequencing method for adventitious virus detection: Demonstration of sensitivity in multiple cell lines.
  In the past few years NGS has become the technology of choice to replace animal-based virus safety methods and this has been strengthened by the recent revision to the ICHQ5A virus safety chapter. Here we describe the validation of an NGS method using an agnos…
  pubmed Bova RA, Diaz L, Eubank S, Overgard A 等 2024 May 置信度 0.82
• Nitrogen starvation causes lipid remodeling in Rhodotorula toruloides.
  The oleaginous yeast Rhodotorula toruloides is a promising chassis organism for the biomanufacturing of value-added bioproducts. It can accumulate lipids at a high fraction of biomass. However, metabolic engineering efforts in this organism have progressed at …
  pubmed Mishra S, Deewan A, Zhao H, Rao CV 2024 May 17 置信度 0.82
• Design, Synthesis, and Bioactivity of Trifluoroethylthio-Substituted Phenylpyrazole Derivatives.
  As the first marketed phenylpyrazole insecticide, fipronil exhibited remarkable broad-spectrum insecticidal activity. However, it poses a significant threat to aquatic organisms and bees due to its high toxicity. Herein, 35 phenylpyrazole derivatives containin…
  pubmed Dong L, Wang W, Zhou L, Yang W 等 2024 May 29 置信度 0.82
• Lineage-Specific Mesenchymal Stromal Cells Derived from Human iPSCs Showed Distinct Patterns in Transcriptomic Profile and Extracellular Vesicle Production.
  Over the past decades, mesenchymal stromal cells (MSCs) have been extensively investigated as a potential therapeutic cell source for the treatment of various disorders. Differentiation of MSCs from human induced pluripotent stem cells (iMSCs) has provided a s…
  pubmed Winston T, Song Y, Shi H, Yang J 等 2024 Jul 置信度 0.82
• How Framing Bias Impacts Preferences for Innovation in Bone Tissue Engineering.
  It is currently unknown if surgeons and biomaterial scientists &or tissue engineers (BS&orTE) process and evaluate information in similar or different (un)biased ways. For the gold standard of surgery to move "from bench to bedside," there must natural…
  pubmed Laubach M, Whyte S, Chan HF, Hildebrand F 等 2025 Mar 置信度 0.82
• Toward microfluidic continuous-flow and intelligent downstream processing of biopharmaceuticals.
  Biopharmaceuticals have emerged as powerful therapeutic agents, revolutionizing the treatment landscape for various diseases, including cancer, infectious diseases, autoimmune and genetic disorders. These biotherapeutics pave the way for precision medicine wit…
  pubmed Sharma V, Mottafegh A, Joo JU, Kang JH 等 2024 May 28 置信度 0.82
• Discovery and characterization of two novel polyethylene terephthalate hydrolases: One from a bacterium identified in human feces and one from the Streptomyces genus.
  Polyethylene terephthalate (PET) is widely used for various industrial applications. However, owing to its extremely slow breakdown rate, PET accumulates as plastic trash, which negatively affects the environment and human health. Here, we report two novel PET…
  pubmed Han Z, Nina MRH, Zhang X, Huang H 等 2024 Jul 5 置信度 0.82
• Recombinant manufacturing of multispecies biolubricants.
  Lubricin, a lubricating glycoprotein abundant in synovial fluid, forms a low-friction brush polymer interface in tissues exposed to sliding motion including joints, tendon sheaths, and the surface of the eye. Despite its therapeutic potential in diseases such …
  pubmed Colville MJ, Huang LT, Schmidt S, Chen K 等 2024 May 5 置信度 0.82
• Effects of different extrusion temperatures on the physicochemical properties, edible quality and digestive attributes of multigrain reconstituted rice.
  Multigrain reconstituted rice, as a nutritious and convenient staple, holds considerable promise for the food industry. Furthermore, highland barley, corn, and other coarse cereals are distinguished by their low glycemic index (GI), rendering them effective in…
  pubmed Liu R, Geng Z, Li T, Zhang M 等 2024 Jun 4 置信度 0.82
• Corrigendum to "Characterization of a novel live attenuated Edwardsiella piscicida vaccine based on the overexpressed type III secretion system and systematic deletion of the associated effectors" [Fish Shellfish Immunol. 106 (2020) 536-545].
  pubmed Yin K, Ma J, Jin P, Sun X 等 2024 Jun 置信度 0.82
• Preparation of (S)-epichlorohydrin using a novel halohydrin dehalogenase by selective conformation adjustment.
  Chiral epichlorohydrin (ECH) is an attractive intermediate for chiral pharmaceuticals and chemicals preparation. The asymmetric synthesis of chiral ECH using 1,3-dicholoro-2-propanol (1,3-DCP) catalyzed by a haloalcohol dehalogenase (HHDH) was considered as a …
  pubmed Zhang XJ, Huang MY, Peng XX, Cao M 等 2024 Aug 置信度 0.82
• Microbial chassis as the platform for production of dihydroxy xanthophyll-based carotenoids: an overview of recent advances in biomanufacturing.
  Physiological and environmental cues prompt microbes to synthesize diverse carotenoids, including dihydroxy xanthophylls, facilitating their adaptation and survival. Lutein and its isomeric counterpart, zeaxanthin, are notable dihydroxy xanthophylls with bioac…
  pubmed Makaranga A, Nesamma AA, Jutur PP 2024 May 9 置信度 0.82
• Complete biosynthesis of QS-21 in engineered yeast.
  QS-21 is a potent vaccine adjuvant and remains the only saponin-based adjuvant that has been clinically approved for use in humans 1,2 . However, owing to the complex structure of QS-21, its availability is limited. Today, the supply depends on laborious extra…
  pubmed Liu Y, Zhao X, Gan F, Chen X 等 2024 May 置信度 0.82
• Mining novel gene targets for improving tolerance to furfural and acetic acid in Yarrowia lipolytica using whole-genome CRISPRi library.
  Abundant renewable resource lignocellulosic biomass possesses tremendous potential for green biomanufacturing, while its efficient utilization by Yarrowia lipolytica, an attractive biochemical production host, is restricted since the presence of inhibitors fur…
  pubmed Fang L, Chen Y, He Q, Wang L 等 2024 Jul 置信度 0.82