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• Label-free metabolic optical biomarkers track stem cell fate transition in real time.
  Tracking stem cell fate transition is crucial for understanding their development and optimizing biomanufacturing. Destructive single-cell methods provide a pseudotemporal landscape of stem cell differentiation but cannot monitor stem cell fate in real time. W…
  pubmed Zhou H, Li I, Bramlett CS, Wang B 等 2024 May 10 置信度 0.82
• Identification and characterization of a TLR4 homologue in Eriocheir sinensis based on structure analysis.
  Toll-like receptor 4 (TLR4) plays an essential role in the activation of innate immunity by recognizing diverse pathogenic components of bacteria. Six Tolls were found in Eriocheir sinensis but have not yet been identified as mammalian TLR4 homolog. For this p…
  pubmed Shi C, Jin Z, Yu Y, Tang Z 等 2024 Aug 置信度 0.82
• Enhanced extracellular production of laccase in Coprinopsis cinerea by silencing chitinase gene.
  Laccase, a copper-containing polyphenol oxidase, is an important green biocatalyst. In this study, Laccase Lcc5 was homologous recombinantly expressed in Coprinopsis cinerea and a novel strategy of silencing chitinase gene expression was used to enhance recomb…
  pubmed Yao D, Ma Y, Ran J, Wang J 等 2024 May 7 置信度 0.82
• A comprehensive review on the recent advances for 5-aminolevulinic acid production by the engineered bacteria.
  5-Aminolevulinic acid (5-ALA) is a non-proteinogenic amino acid essential for synthesizing tetrapyrrole compounds, including heme, chlorophyll, cytochrome, and vitamin B12. As a plant growth regulator, 5-ALA is extensively used in agriculture to enhance crop y…
  pubmed Chen YY, Huang JC, Wu CY, Yu SQ 等 2025 Feb 置信度 0.82
• Surfactant-mediated bio-manufacture: A unique strategy for promoting microbial biochemicals production.
  Biochemicals are widely used in the medicine and food industries and are more efficient and safer than synthetic chemicals. The amphipathic surfactants can interact with the microorganisms and embed the extracellular metabolites, which induce microbial metabol…
  pubmed Yi Y, Jin X, Chen M, Coldea TE 等 2024 Jul-Aug 置信度 0.82
• Characterisation of aroma compounds, sensory characteristics, and bioactive components of a new type of huangjiu fermented with Chinese wild rice (Zizania latifolia).
  Chinese wild rice (CWR) is a nutritious and healthy whole grain, worth developing. To develop and use its value, a new type of huangjiu was brewed with CWR, and the flavour characteristics, sensory quality, functional and bioactive components were evaluated. C…
  pubmed Zhao Y, Gu M, Jiang P, Fang S 等 2024 Sep 15 置信度 0.82
• Decellularised plant scaffolds facilitate porcine skeletal muscle tissue engineering for cultivated meat biomanufacturing.
  Cultivated meat (CM) offers a sustainable and ethical alternative to conventional animal agriculture, involving cell maturation in a controlled environment. To emulate the structural complexity of traditional meat, the development of animal-free and edible sca…
  pubmed Murugan P, Yap WS, Ezhilarasu H, Suntornnond R 等 2024 May 3 置信度 0.82
• Reprogramming anchorage dependency to develop cell lines for recombinant protein expression.
  As the biopharmaceutical industry continues to mature in its cost-effectiveness and productivity, many companies have begun employing larger-scale biomanufacturing and bioprocessing protocols. While many of these protocols require cells with anchorage-independ…
  pubmed Lee JY, Huh HD, Lee DK, Park SY 等 2024 May 置信度 0.82
• Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence.
  Manufacturing chimeric antigen receptor (CAR) T cell therapies is complex, with limited understanding of how medium composition impacts T cell phenotypes. CRISPR-Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting the endogen…
  pubmed Cappabianca D, Pham D, Forsberg MH, Bugel M 等 2024 Jun 13 置信度 0.82
• Disease related changes in ATAC-seq of iPSC-derived motor neuron lines from ALS patients and controls.
  Amyotrophic Lateral Sclerosis (ALS), like many other neurodegenerative diseases, is highly heritable, but with only a small fraction of cases explained by monogenic disease alleles. To better understand sporadic ALS, we report epigenomic profiles, as measured …
  pubmed Tsitkov S, Valentine K, Kozareva V, Donde A 等 2024 May 2 置信度 0.82
• Enhancing the activity and succinyl-CoA specificity of 3-ketoacyl-CoA thiolase Tfu_0875 through rational binding pocket engineering.
  The 3-ketoacyl-CoA thiolase is the rate-limiting enzyme for linear dicarboxylic acids production. However, the promiscuous substrate specificity and suboptimal catalytic performance have restricted its application. Here we present both biochemical and structur…
  pubmed Liu L, Liu S, Hu X, Zhou S 等 2024 Sep 置信度 0.82
• Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms.
  Recombinant adeno-associated viruses (rAAVs) stand at the forefront of gene therapy applications, holding immense significance for their safe and efficient gene delivery capabilities. The constantly increasing and unmet demand for rAAVs underscores the need fo…
  pubmed Catalán-Tatjer D, Tzimou K, Nielsen LK, Lavado-García J 2024 Jul-Aug 置信度 0.82
• In Vitro Evaluation of Pore Size Graded Bone Scaffolds with Different Material Composition.
  The demand for biomimetic and biocompatible scaffolds in equivalence of structure and material composition for the regeneration of bone tissue is relevantly high. This article is investigating a novel three-dimensional (3D) printed porous structure called bone…
  pubmed Daskalakis E, Huang B, Hassan MH, Omar AM 等 2024 Apr 1 置信度 0.82
• Acidification induce chemical and microbial variation in tea plantation soils and bacterial degradation of the key acidifying phenolic acids.
  Camellia sinensis is an important economic plant grown in southern subtropical hilly areas, especially in China, mainly for the production of tea. Soil acidification is a significant cause of the reduction of yield and quality and continuous cropping obstacles…
  pubmed Zhang Y, Wang B, Wang G, Zheng Z 等 2024 Apr 30 置信度 0.82
• Ribosome Pool Engineering Increases Protein Biosynthesis Yields.
  The biosynthetic capability of the bacterial ribosome motivates efforts to understand and harness sequence-optimized versions for synthetic biology. However, functional differences between natively occurring ribosomal RNA (rRNA) operon sequences remain poorly …
  pubmed Kofman C, Willi JA, Karim AS, Jewett MC 2024 Apr 24 置信度 0.82
• Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16.
  Industrial biomanufacturing of value-added products using CO 2 as a carbon source is considered more sustainable, cost-effective and resource-efficient than using common carbohydrate feedstocks. Cupriavidus necator H16 is a representative H 2 -oxidizing lithoa…
  pubmed Jang Y, Lee YJ, Gong G, Lee SM 等 2024 Apr 27 置信度 0.82
• Identification of hyperthermophilic D-allulose 3-epimerase from Thermotoga sp. and its application as a high-performance biocatalyst for D-allulose synthesis.
  D-Allulose 3-epimerase (DAE) is a vital biocatalyst for the industrial synthesis of D-allulose, an ultra-low calorie rare sugar. However, limited thermostability of DAEs hinders their use at high-temperature production. In this research, hyperthermophilic TI-D…
  pubmed Shen JD, Xu BP, Yu TL, Fei YX 等 2024 Jun 置信度 0.82
• The Preparation and Characterization of Quinoa Protein Gels and Application in Eggless Bread.
  The properties of xanthan gum protein gels composed of quinoa protein (XG-QPG) and ultrasound-treated quinoa protein (XG-UQPG) were compared for the preparation of high-quality quinoa protein gels. The gel qualities at different pH values were compared. The ge…
  pubmed Xu Q, Zhang X, Zuo Z, Zhang M 等 2024 Apr 21 置信度 0.82
• Redox active plant phenolic, acetosyringone, for electrogenetic signaling.
  Redox is a unique, programmable modality capable of bridging communication between biology and electronics. Previous studies have shown that the E. coli redox-responsive OxyRS regulon can be re-wired to accept electrochemically generated hydrogen peroxide (H 2…
  pubmed Zakaria FR, Chen CY, Li J, Wang S 等 2024 Apr 26 置信度 0.82
• Continuous catalytic production of 1,3-dihydroxyacetone: Sustainable approach combining perfusion cultures and immobilized cells.
  Currently, the predominant method for the industrial production of 1,3-dihydroxyacetone (DHA) from glycerol involves fed-batch fermentation. However, previous research has revealed that in the biocatalytic synthesis of DHA from glycerol, when the DHA concentra…
  pubmed Sun Y, Liu T, Nie J, Yan J 等 2024 Jun 置信度 0.82
• Perspective on Lignin Conversion Strategies That Enable Next Generation Biorefineries.
  The valorization of lignin, a currently underutilized component of lignocellulosic biomass, has attracted attention to promote a stable and circular bioeconomy. Successful approaches including thermochemical, biological, and catalytic lignin depolymerization h…
  pubmed Shrestha S, Goswami S, Banerjee D, Garcia V 等 2024 Aug 26 置信度 0.82
• Temperature modulated sustainable on/off photosynthesis switching of microalgae towards hydrogen evolution.
  Despite great progress in the active interfacing between various abiotic materials and living organisms, the development of a smart polymer matrix with modulated functionality of algae towards the application of green bioenergy is still rare. Herein, we design…
  pubmed Li S, Xu Z, Lin S, Li L 等 2024 Apr 24 置信度 0.82
• A Self-Assembled MOF-Escherichia Coli Hybrid System for Light-Driven Fuels and Valuable Chemicals Synthesis.
  The development of semi-artificial photosynthetic systems, which integrate metal-organic frameworks (MOFs) with industrial microbial cell factories for light-driven synthesis of fuels and valuable chemicals, represents a highly promising avenue for both resear…
  pubmed Li J, Shen J, Hou T, Tang H 等 2024 Jul 置信度 0.82
• Oxygen regulation of microbial communities and chemical compounds in cigar tobacco curing.
  Curing is a critical process that determines the sensory quality of cigars. The impact of oxygen on cigar curing and the mechanisms by which it regulates microbial changes affecting cigar quality are not well understood.
  pubmed Yang J, Xue F, Li D, Chen J 等 2024 置信度 0.82
• Development of Leptolyngbya sp. BL0902 into a model organism for synthetic biological research in filamentous cyanobacteria.
  Cyanobacteria have great potential in CO 2 -based bio-manufacturing and synthetic biological studies. The filamentous cyanobacterium, Leptolyngbya sp. strain BL0902, is comparable to Arthrospira ( Spirulina ) platensis in commercial-scale cultivation while pro…
  pubmed Gao H, Wang Y, Huang Z, Yu F 等 2024 置信度 0.82
• Synthesis of glucosamine-selenium compound and evaluation of its oral toxicity and in vitro anti-hepatitis B virus activity.
  Selenium supplements are beneficial to human health, however, concerns regarding the toxicity of inorganic selenium have stimulated research on safer organic compounds. The main objective of this study was to develop a novel glucosamine-selenium compound (Se-G…
  pubmed Ding H, Lu X, Ji X, Wang S 等 2024 Oct 1 置信度 0.82
• Gene circuit-based sensors.
  As an important branch of biosensors, gene circuit-based biosensors constructed based on synthetic biology concepts can sense specific substances to be measured and converted into specific signal outputs. Such sensors have great potential in the fields of biom…
  pubmed Guo X, Li M, Zuo X 2025 Sep 置信度 0.82
• Oriented cellulose hydrogel: Directed tissue regeneration for reducing corneal leukoplakia and managing fungal corneal ulcers.
  Fungal corneal ulcer is one of the leading causes of corneal blindness in developing countries. Corneal scars such as leukoplakia are formed due to inflammation, oxidative stress and non-directed repair, which seriously affect the patients' subsequent visual a…
  pubmed Dong L, Fan Z, Fang B, Zhao X 等 2024 Nov 置信度 0.82
• Well Plate-Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery.
  Efficient and nontoxic delivery of foreign cargo into cells is a critical step in many biological studies and cell engineering workflows with applications in areas such as biomanufacturing and cell-based therapeutics. However, effective molecular delivery into…
  pubmed Patino CA, Sarikaya S, Mukherjee P, Pathak N 等 2024 Jul 20 置信度 0.82
• Peptide ligands for the universal purification of exosomes by affinity chromatography.
  Exosomes are gaining prominence as vectors for drug delivery, vaccination, and regenerative medicine. Owing to their surface biochemistry, which reflects the parent cell membrane, these nanoscale biologics feature low immunogenicity, tunable tissue tropism, an…
  pubmed Kilgore RE, Moore BD, Sripada SA, Chu W 等 2024 Nov 置信度 0.82
• Phosphatidylserine: A comprehensive overview of synthesis, metabolism, and nutrition.
  Phosphatidylserine (PtdS) is classified as a glycerophospholipid and a primary anionic phospholipid and is particularly abundant in the inner leaflet of the plasma membrane in neural tissues. It is synthesized from phosphatidylcholine or phosphatidylethanolami…
  pubmed Hussain M, Khan I, Chaudhary MN, Ali K 等 2024 Oct 置信度 0.82
• Embedding Biomimetic Vascular Networks via Coaxial Sacrificial Writing into Functional Tissue.
  Printing human tissues and organs replete with biomimetic vascular networks is of growing interest. While it is possible to embed perfusable channels within acellular and densely cellular matrices, they do not currently possess the biomimetic architectures fou…
  pubmed Stankey PP, Kroll KT, Ainscough AJ, Reynolds DS 等 2024 Sep 置信度 0.82
• A Light-Powered In Vitro Synthetic Enzymatic Biosystem for the Synthesis of 3-Hydroxypropionic Acid via CO(2) Fixation.
  3-Hydroxypropionic acid (3-HP) is a highly sought-after platform chemical serving as a precursor to a variety of high value-added chemical products. In this study, we designed and constructed a novel light-powered in vitro synthetic enzymatic biosystem compris…
  pubmed Ning X, Li F, Wei X, Zhu Z 等 2024 Aug 16 置信度 0.82
• Evaluating the feasibility of medium-chain oleochemical synthesis using microbial chain elongation.
  Chain elongating bacteria are a unique guild of strictly anaerobic bacteria that have garnered interest for sustainable chemical manufacturing from carbon-rich wet and gaseous waste streams. They produce C6-C8 medium-chain fatty acids, which are valuable platf…
  pubmed Agena E, Gois IM, Bowers CM, Mahadevan R 等 2024 Jan 9 置信度 0.82
• Continuous Flow Microreactors for the High-efficiency Enzymatic Synthesis of 10-Hydroxystearic Acid from Oleic Acid.
  Converting fatty acids into specialty chemicals is sustainable but hindered by the low efficiency and thermal instability of current oleic acid hydratases, along with mass transfer limitations in emulsion reactions. This study introduces an optimized continuou…
  pubmed Zhao YF, Xie WL, He K, Li HP 等 2024 Oct 16 置信度 0.82
• Preparation of High-Purity Docosahexaenoic Acid Ethyl Ester from Algal Oil through Enzymatic Ethanolysis.
  Docosahexaenoic acid plays a crucial role in infant brain function, and the market demand of high-purity docosahexaenoic acid is continuously increasing. The availability of docosahexaenoic acid in natural fish oil is limited, prompting the exploration of alte…
  pubmed Li J, Lin S, Liu X, Chen W 等 2024 置信度 0.82
• Development of a Type I-E CRISPR-Based Programmable Repression System for Fine-Tuning Metabolic Flux toward D-Pantothenic Acid in Bacillus subtilis.
  The CRISPR-based regulation tools enable fine-tuning of gene transcription, showing potential in areas of biomanufacturing and live therapeutics. However, the cell toxicity and PAM specificity of existing CRISPR-based regulation systems limit their broad appli…
  pubmed Mao C, Zheng H, Chen Y, Yuan P 等 2024 Aug 16 置信度 0.82
• A modular toolkit for environmental Rhodococcus, Gordonia, and Nocardia enables complex metabolic manipulation.
  Soil-dwelling Actinomycetes are a diverse and ubiquitous component of the global microbiome but largely lack genetic tools comparable to those available in model species such as Escherichia coli or Pseudomonas putida , posing a fundamental barrier to their cha…
  pubmed Jansen Z, Alameri A, Wei Q, Kulhanek DL 等 2024 Aug 21 置信度 0.82
• Engineering bacterial biomanufacturing: characterization and manipulation of Sphingomonas sp. LM7 extracellular polymers.
  Biologically produced materials are an attractive alternative to traditional materials such as metals and plastics and offer improved functionalities such as better biodegradability and biocompatibility. Polysaccharides are an example of biologically produced …
  pubmed van Wijngaarden EW, Goetsch AG, Brito IL, Hershey DM 等 2024 Aug 14 置信度 0.82
• Robust bioprocess design and evaluation of commercial media for the serial expansion of human induced pluripotent stem cell aggregate cultures in vertical-wheel bioreactors.
  While pluripotent stem cell (PSC) therapies move toward clinical and commercial applications at a rapid rate, manufacturing reproducibility and robustness are notable bottlenecks in regulatory approval. Therapeutic applications of PSCs require large cell quant…
  pubmed Borys BS, Dang T, Worden H, Larijani L 等 2024 Jul 29 置信度 0.82
• A quantitative image analysis platform for assessing trophoblast differentiation.
  Immunofluorescence microscopy is extensively used in characterization of trophoblast differentiation in vitro. However, such data is primarily used to confirm the presence of protein markers or qualitatively compare levels of protein markers across experimenta…
  pubmed Jabeen M, Karakis V, Britt J, Miguel AS 等 2025 Jun 13 置信度 0.82
• Sustainable biofabrication: from bioprinting to AI-driven predictive methods.
  Biofabrication is potentially an inherently sustainable manufacturing process of bio-hybrid systems based on biomaterials embedded with cell communities. These bio-hybrids promise to augment the sustainability of various human activities, ranging from tissue e…
  pubmed Filippi M, Mekkattu M, Katzschmann RK 2025 Feb 置信度 0.82
• Hydrogel Loaded with Extracellular Vesicles: An Emerging Strategy for Wound Healing.
  An increasing number of novel biomaterials have been applied in wound healing therapy. Creating beneficial environments and containing various bioactive molecules, hydrogel- and extracellular vesicle (EV)-based therapies have respectively emerged as effective …
  pubmed Yang Y, Chen H, Li Y, Liang J 等 2024 Jul 10 置信度 0.82
• The Undeniable Potential of Thermophiles in Industrial Processes.
  Extremophilic microorganisms play a key role in understanding how life on Earth originated and evolved over centuries. Their ability to thrive in harsh environments relies on a plethora of mechanisms developed to survive at extreme temperatures, pressures, sal…
  pubmed Gallo G, Imbimbo P, Aulitto M 2024 Jul 13 置信度 0.82
• Shh Gene Regulates the Proliferation and Apoptosis of Dermal Papilla Cells to Affect Its Differential Expression in Secondary Hair Follicle Growth Cycle of Cashmere Goats.
  Sonic hedgehog (Shh) is a component of the Hedgehog signaling pathway, playing an important role in regulating cell proliferation, differentiation, apoptosis, and the repair of damaged organisms. To further clarify the expression pattern of Shh gene in the sec…
  pubmed Zhang J, Liu Y, Chang J, Zhang R 等 2024 Jul 12 置信度 0.82
• Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control.
  Nanobodies are single-domain antibody fragments that have garnered considerable use as diagnostic and therapeutic agents as well as research tools. However, obtaining pure VHHs, like many proteins, can be laborious and inconsistent. High level cytoplasmic expr…
  pubmed Hennigan JN, Menacho-Melgar R, Sarkar P, Golovsky M 等 2024 Sep 置信度 0.82
• The feasibility and safety of biomaterials for posterior scleral reinforcement in rabbits.
  To explore the feasibility and safety of biomaterials for posterior scleral reinforcement (PSR) in rabbits. Decellularization and genipin crosslink were applied to the fresh bovine pericardium and porcine endocranium, and then mechanical properties, suture ret…
  pubmed Zhang WF, Li BH, Liu ZB, Peng TR 等 2024 Aug 8 置信度 0.82
• The Impact of Novel BMPR1B Mutations on Litter Size in Short-Tailed Gobi Sheep and Larger-Tailed Ujimqin Sheep.
  The significant deposition of tail fat in sheep has a profound impact on the economic benefits of animal husbandry. Furthermore, increasing the litter size is a crucial means of enhancing economic benefits. The BMPR1B and T / Brachyury genes are considered maj…
  pubmed Bai Y, Wang S, Wu K, Zhang M 等 2024 Jul 1 置信度 0.82
• Learning metabolic dynamics from irregular observations by Bidirectional Time-Series State Transfer Network.
  Modeling microbial metabolic dynamics is important for the rational optimization of both biosynthetic systems and industrial processes to facilitate green and efficient biomanufacturing. Classical approaches utilize explicit equation systems to represent metab…
  pubmed Xu S, Xu T, Yang Y, Chen X 2024 Aug 20 置信度 0.82
• Considerations for oral and dental tissues in holistic care during long-haul space flights.
  The health of astronauts during and after the return from long-haul space missions is paramount. There is plethora of research in the literature about the medical side of astronauts' health, however, the dental and oral health of the space crew seem to be over…
  pubmed Bakr MM, Caswell GM, Hussein H, Shamel M 等 2024 置信度 0.82
• Reprogramming naturally evolved switches for Streptomyces chassis development.
  The Streptomyces chassis serves as an important platform for efficient biomanufacture of diverse secondary metabolite (SM) compounds, but the current chassis lacks compatibility for integration of these SM biosynthetic pathways reliably and consistently. This …
  pubmed Yan H, Li S, Wang W 2025 Jan 置信度 0.82
• Multicopper Oxidase from Lactobacillus hilgardii: Mechanism of Degradation of Tyramine and Phenylethylamine in Fermented Food.
  Elevated levels of biogenic amines (BAs) in fermented food can have negative effects on both the flavor and health. Mining enzymes that degrade BAs is an effective strategy for controlling their content. The study screened a strain of Lactobacillus hilgardii 1…
  pubmed Yang Q, Bai Y, Liu S, Han X 等 2024 Aug 7 置信度 0.82
• Design considerations and biomaterials selection in embedded extrusion 3D bioprinting.
  In embedded extrusion 3D bioprinting, a temporary matrix preserves a paste-like filament ejecting from a narrow nozzle. For granular sacrificial matrices, the methodology is known as the freeform reversible embedding of suspended hydrogels (FRESH). Embedded ex…
  pubmed Yogeshwaran S, Goodarzi Hosseinabadi H, Gendy DE, Miri AK 2024 Sep 10 置信度 0.82
• Balancing the AspC and AspA Pathways of Escherichia coli by Systematic Metabolic Engineering Strategy for High-Efficient l-Homoserine Production.
  l-Homoserine is a promising C4 platform compound used in the agricultural, cosmetic, and pharmaceutical industries. Numerous works have been conducted to engineer Escherichia coli to be an excellent l-homoserine producer, but it is still unable to meet the ind…
  pubmed Chen Y, Huang L, Yu T, Yao Y 等 2024 Aug 16 置信度 0.82
• Optical redox imaging to screen synthetic hydrogels for stem cell-derived cardiomyocyte differentiation and maturation.
  Heart disease is the leading cause of death in the United States, yet research is limited by the inability to culture primary cardiac cells. Cardiomyocytes (CMs) derived from human induced pluripotent stem cells (iPSCs) are a promising solution for drug screen…
  pubmed Desa DE, Amitrano MJ, Murphy WL, Skala MC 2024 May 置信度 0.82
• Revealing reaction intermediates in one-carbon elongation by thiamine diphosphate/CoA-dependent enzyme family.
  2-Hydroxyacyl-CoA lyase/synthase (HACL/S) is a thiamine diphosphate (ThDP)-dependent versatile enzyme originally discovered in the mammalian α-oxidation pathway. HACL/S natively cleaves 2-hydroxyacyl-CoAs and, in its reverse direction, condenses formyl-C…
  pubmed Kim Y, Lee SH, Gade P, Nattermann M 等 2024 Jul 21 置信度 0.82
• Roles of α-1,3-glucosyltransferase in growth and polysaccharides biosynthesis of Ganoderma lucidum.
  Ganoderma lucidum polysaccharides are valuable natural compounds possessing significant biological activity, with glycosyltransferases playing a crucial role in their biosynthesis. Although the function of β-1,3-glucosyltransferase in polysaccharides pro…
  pubmed Chen H, Zhao L, You C, Liu J 等 2024 Sep 置信度 0.82
• Unraveling the intricacies of glycosaminoglycan biosynthesis: Decoding the molecular symphony in understanding complex polysaccharide assembly.
  Glycosaminoglycans (GAGs) are extensively utilized in clinical, cosmetic, and healthcare field, as well as in the treatment of thrombosis, osteoarthritis, rheumatism, and cancer. The biological production of GAGs is a strategy that has garnered significant att…
  pubmed Yao ZY, Gong JS, Jiang JY, Su C 等 2024 Oct 置信度 0.82
• Bacillus methanolicus: an emerging chassis for low-carbon biomanufacturing.
  Bacillus methanolicus is a thermophilic methylotrophic bacterium that grows quickly on methanol in sea water-based media. It has been engineered for chemical bioproduction from methanol, but its efficiency needs improvement for industrialization. Synthetic bio…
  pubmed Yang X, Zheng Z, Wang Y 2025 Feb 置信度 0.82
• Advances in serum-free media for CHO cells: From traditional serum substitutes to microbial-derived substances.
  The Chinese hamster ovary (CHO) cell is an epithelial-like cell that produces proteins with post-translational modifications similar to human glycosylation. It is widely used in the production of recombinant therapeutic proteins and monoclonal antibodies. Cult…
  pubmed Zhang M, Zhao X, Li Y, Ye Q 等 2024 Jun 置信度 0.82
• 3D Printed Organisms Enabled by Aspiration-Assisted Adaptive Strategies.
  Devising an approach to deterministically position organisms can impact various fields such as bioimaging, cybernetics, cryopreservation, and organism-integrated devices. This requires continuously assessing the locations of randomly distributed organisms to c…
  pubmed Han G, Khosla K, Smith KT, Ng DWH 等 2024 Aug 置信度 0.82
• A novel phosphotriesterase hybrid nanoflower-hydrogel sensor equipped with a smartphone detector for real-time on-site monitoring of organophosphorus pesticides.
  Designing efficient and rapid methods for the detection of organophosphorus pesticides (OPs) residue is a prerequisite to mitigate their negative health impacts. In this study, we propose the concept of an enzyme catalysis system-based hydrogel kit integrated …
  pubmed Chen J, Hao M, Xin Y, Zhu R 等 2024 Sep 置信度 0.82
• Combinatorial strain improvement and bioprocess development for efficient production of ε-poly-L-lysine in Streptomyces albulus.
  ε-Poly-L-lysine (ε-PL) is an amino acid homopolymer with diverse potential applications in the food, pharmaceutical and cosmetic industries. To improve its biomanufacturing efficiency, strain engineering and bioprocess optimization were combined in…
  pubmed Zhu D, Liu Y, Yang H, Zhang J 等 2024 Sep 置信度 0.82
• Antibacterial, mosquito larvicidal, and cytotoxicity potential of AgNPs synthesized using Pittosporum undulatum under in vitro conditions.
  In this study, the phytochemical profile and silver nanoparticle (AgNPs)-synthesizing ability of Pittosporum undulatum methanol extract were investigated. Furthermore, biological applications of the AgNPs, such as antibacterial effect (against Klebsiella pneum…
  pubmed Narayanan M, Alshiekheid MA, Saravanan M 2024 Nov 1 置信度 0.82
• Development and application of a 1K functional liquid chip for lactation performance in Bactrian camels.
  The advancement of high-throughput, high-quality, flexible, and cost-effective genotyping platforms is crucial for the progress of dairy breeding in Bactrian camels. This study focuses on developing and evaluating a 1K functional liquid single nucleotide polym…
  pubmed Guo L, Dao L, Liu B, Wang J 等 2024 置信度 0.82
• Development of a marker recyclable CRISPR/Cas9 system for scarless and multigene editing in Fusarium fujikuroi.
  Iterative metabolic engineering of Fusarium fujikuroi has traditionally been hampered by its low homologous recombination efficiency and scarcity of genetic markers. Thus, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated…
  pubmed Huang L, Li N, Song Y, Gao J 等 2024 Jul 置信度 0.82
• Through virtual saturation mutagenesis and rational design for superior substrate conversion in engineered d-amino acid oxidase.
  The d-amino acid oxidase (DAAO) is pivotal in obtaining optically pure l-glufosinate (l-PPT) by converting d-glufosinate (d-PPT) to its deamination product. We screened and designed a Rasamsonia emersonii DAAO (ReDAAO), making it more suitable for oxidizing d-…
  pubmed Tang H, Zhu HL, Zhao JQ, Wang LY 等 2024 Jul 置信度 0.82
• Synthetic biology for Monascus: From strain breeding to industrial production.
  Traditional Chinese food therapies often motivate the development of modern medicines, and learning from them will bring bright prospects. Monascus, a conventional Chinese fungus with centuries of use in the food industry, produces various metabolites, includi…
  pubmed Zhou J, Pan Q, Xue Y, Dong Y 等 2024 Jul 置信度 0.82
• Metabolic engineering of Komagataella phaffii for the efficient utilization of methanol.
  Komagataella phaffii, a type of methanotrophic yeast, can use methanol, a favorable non-sugar substrate in eco-friendly bio-manufacturing. The dissimilation pathway in K. phaffii leads to the loss of carbon atoms in the form of CO 2 . However, the ΔFLD s…
  pubmed Wang Y, Li R, Zhao F, Wang S 等 2024 Jul 17 置信度 0.82
• A Genomewide Evolution-Based CRISPR/Cas9 with Donor-Free (GEbCD) for Developing Robust and Productive Industrial Yeast.
  Developing more robust and productive industrial yeast is crucial for high-efficiency biomanufacturing. However, the challenges posed by the long time required and the low abundance of mutations generated through genomewide evolutionary engineering hinder the …
  pubmed Zhang J, Zhao G, Bai W, Chen Y 等 2024 Aug 16 置信度 0.82
• Stepwise Reduction of Redox Noninnocent Nitrosobenzene to Aniline via a Rare Phenylhydroxylamino Intermediate on a Thiolate-Bridged Dicobalt Scaffold.
  Nitrosobenzene (PhNO) and phenylhydroxylamine (PhNHOH) are of paramount importance because of their involvement as crucial intermediates in the biological metabolism and catalytic transformation of nitrobenzene (PhNO 2 ) to aniline (PhNH 2 ). However, a comple…
  pubmed Wang Z, Zhang H, Zhang P, Di K 等 2024 Jul 24 置信度 0.82
• Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris.
  Pichia pastoris , a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P . pastoris cell factory is somewhat impeded due to the absence…
  pubmed Ruan S, Yang Y, Zhang X, Luo G 等 2024 Dec 置信度 0.82
• Engineering microbial metabolic homeostasis for chemicals production.
  Microbial-based bio-refining promotes the development of a biotechnology revolution to encounter and tackle the enormous challenges in petroleum-based chemical production by biomanufacturing, biocomputing, and biosensing. Nevertheless, microbial metabolic home…
  pubmed Li Y, Liu M, Yang C, Fu H 等 2025 Mar 置信度 0.82
• Voxelated bioprinting of modular double-network bio-ink droplets.
  Analogous of pixels to two-dimensional pictures, voxels-in the form of either small cubes or spheres-are the basic building blocks of three-dimensional objects. However, precise manipulation of viscoelastic bio-ink voxels in three-dimensional space represents …
  pubmed Zhu J, He Y, Wang Y, Cai LH 2024 Jul 13 置信度 0.82
• Identification of Novel GANT61 Analogs with Activity in Hedgehog Functional Assays and GLI1-Dependent Cancer Cells.
  Aberrant activation of hedgehog (Hh) signaling has been implicated in various cancers. Current FDA-approved inhibitors target the seven-transmembrane receptor Smoothened, but resistance to these drugs has been observed. It has been proposed that a more promisi…
  pubmed Abu Rabe D, Chdid L, Lamson DR, Laudeman CP 等 2024 Jun 28 置信度 0.82
• Natural Bioactive Compounds in the Management of Periodontal Diseases: A Comprehensive Review.
  Periodontal diseases, chronic inflammatory conditions affecting oral health, are primarily driven by microbial plaque biofilm and the body's inflammatory response, leading to tissue damage and potential tooth loss. These diseases have significant physical, psy…
  pubmed Hashim NT, Babiker R, Rahman MM, Mohamed R 等 2024 Jun 26 置信度 0.82
• Design for Additive Manufacturing Constrained Topology Optimization of Liquid-to-Air Heat Exchangers With ML-Based Turbulent Flow Considerations
  Abstract Liquid-to-air heat exchangers are devices that transfer thermal energy from a liquid to the surrounding air and are widely used in different industries. Traditional shell-and-tube or plate-and-fin type heat exchangers have designs that are generally m…
  crossref Botao Zhang, Vysakh Venugopal, Mitansh Tripathi, Navaneeth Chandran 等 2024-08-20T17:56:33Z 置信度 0.70
• “Talent, Talent, Talent” Drives Fujifilm Diosynth's $3.2B NC Expansion
  crossref Alex Philippidis 2024-07-01T16:06:40Z 置信度 0.70
• Analysis of fatty acid methyl esters and polyhydroxyalkanoates production along with wastewater contaminant removal using sewage sludge bacteria
  crossref Asmita Gupta, Madan Kumar, Vivek Kumar, Indu Shekhar Thakur 2024-03-18T15:02:40Z 置信度 0.70
• Protease hydrolysed brewers’ spent grain bioactive extracts for applications in human health
  Abstract About 39 million tons of Brewer’s spent grain (BSG), the main by-product of beer manufacturing, are produced annually and is used for low value applications. To valorise this rich bioresource, the present work entails bioprocessing of BSG with various…
  crossref Azza Silotry Naik, Mila Brugiere, Kevin Dunne, Michael Sorohan 等 2024-03-25T18:02:15Z 置信度 0.70
• Meta-analysis Driven Strain Design for Mitigating Oxidative Stresses Important in Biomanufacturing
  As the availability of data sets increases, meta-analysis leveraging aggregated and interoperable data types is proving valuable. This study leveraged a meta-analysis workflow to identify mutations that could improve robustness to reactive oxygen species (ROS)…
  pubmed PV Phaneuf, SH Kim, K Rychel, C Rode 等 2024 Jul 19 置信度 0.82
• Top-down and bottom-up microbiome engineering approaches to enable biomanufacturing from waste biomass
  Abstract Growing environmental concerns and the need to adopt a circular economy have highlighted the importance of waste valorization for resource recovery. Microbial consortia-enabled biotechnologies have made significant developments in the biomanufacturing…
  pubmed Xuejiao Lyu, Mujaheed Nuhu, Pieter Candry, Jenna Wolfanger 等 2024 Jan 9 置信度 0.82
• State-of-art engineering approaches for ameliorated production of microbial lipid
  crossref Subhadeep Mondal, Suman Kumar Halder, Keshab Chandra Mondal 2023-07-03T16:01:40Z 置信度 0.70
• Engineering mammalian cell growth dynamics for biomanufacturing
  crossref Mauro Torres, Dewi Mcconnaughie, Samia Akhtar, Claire E. Gaffney 等 2024-02-06T01:15:57Z 置信度 0.70
• Optimization of protease production using Bacillus velezensis through response surface methodology and investigating its applications as stain remover
  crossref Humaira, Farah Deba, Hafiz Abdullah Shakir, Muhammad Khan 等 2024-07-02T09:02:17Z 置信度 0.70
• Dual-model biomanufacturing of porous biomimetic scaffolds with concentrated growth factors and embedded endothelial vascular channels for bone defect regeneration
  crossref Zhi Cui, Luping Zhou, Junjie Huang, Liang Xu 等 2024-01-23T17:58:33Z 置信度 0.70
• Integrated Biotic–Abiotic Solar Driven NADPH Regeneration Platform in <i>Escherichia coli</i> for Chemical Biomanufacturing Applications
  Abstract The development of a biohybrid bacterium capable of self‐synthesizing photocatalytically active quantum dots (QDs) is reported using the expression of a template protein that dictates the QDs size and properties. This biotic–abiotic light‐harvesting m…
  crossref Oren Bachar, Matan M. Meirovich, Omer Yehezkeli 2023-12-29T03:07:38Z 置信度 0.70
• Chlorella minutissima-assisted silver nanoparticles synthesis and evaluation of its antibacterial activity
  crossref Lakhan Kumar, Lalit Mohan, Raksha Anand, Navneeta Bharadvaja 2023-04-04T13:23:28Z 置信度 0.70
• Brief Paper: Deciphering the Effect of Part Thermal History on Microstructure and Mechanical Properties in Laser Powder Bed Fusion of SS316L
  Abstract The main research goal of this study is to decipher the intercorrelation between process-induced thermal-structure-property relationships of Stainless Steel 316L fabricated by laser powder bed fusion. The objective therein is achieved by explaining an…
  crossref Kaustubh Deshmukh, Alexander Riensche, Ryan J. Lane, Kyle Snyder 等 2024-08-20T17:56:27Z 置信度 0.70
• New perspectives of omega-3 fatty acids from diatoms
  crossref Rashi Tyagi, Rajesh Prasad Rastogi, Olga Babich, Mukesh Kumar Awasthi 等 2023-09-02T15:01:57Z 置信度 0.70
• Online Monitoring of Infrared Pyrometer Captured Thermal Signature for Prediction of Iron Dilution and its Effect on Corrosion Properties of Laser Deposited Inconel 625 Coating on SS 304L
  Abstract The current study deals with understanding the implication of iron (Fe) dilution from underlying stainless steel (SS) substrate (SS304L) on corrosion properties of laser deposited Inconel 625 through insitu/online monitoring of thermal signatures capt…
  crossref Saurav Misra, Ipsita Mohanty, Cheruvu Siva Kumar, Partha Saha 2024-08-20T17:56:13Z 置信度 0.70
• Coenzyme A Thioester Intermediates as Platform Molecules in Cell‐Free Chemical Biomanufacturing
  Abstract The in vitro synthesis of Coenzyme A (CoA)‐thioester intermediates opens new avenues to transform simple molecules into more complex and multifunctional ones by assembling cell‐free biosynthetic cascades. In this review, we have systematically catalog…
  crossref Laurine Ducrot, Idania L. López, Alejandro H. Orrego, Fernando López‐Gallego 2023-11-24T19:11:59Z 置信度 0.70
• Efficient production of (2R, 3R)-butanediol from xylose by an engineered Serratia marcescens
  crossref Tingting Sun, Di Liu, Linmeng Zhang, Taiping Fan 等 2023-11-24T12:02:35Z 置信度 0.70
• Small-scale perfusion mimic cultures in the ambr250 HT bioreactor system
  crossref Srikanth Rapala, Abiageal Barton, Sarah W. Harcum 2024-04-13T13:49:50Z 置信度 0.70
• Viewpoint of multi-omics potential in tuberculosis research: identifying biomarkers for biomanufacturing of efficient control tools
  Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), remains a global health burden, claiming millions of lives annually. Despite the availability of a vaccine (the Bacillus Calmette-Guérin; BCG), diagnostics (smear microscopy and DNA-based …
  crossref Isaac Darko Otchere, Sammy Yaw Aboagye, Patrick Kobina Arthur, Adwoa Asante-Poku 2024-07-31T05:17:31Z 置信度 0.70
• Response surface methodology (RSM) and artificial neural network (ANN) integrated optimization for lipase production by Bacillus holotolerans
  crossref Veeranna Shivaputrayya Hombalimath, Dummi Mahadevan Gurumurthy 2023-11-21T20:02:18Z 置信度 0.70
• Melt electrowriting on out-of-plane surfaces: The challenge of controlling the electric field for textile biomanufacturing
  crossref Javier Vazquez-Armendariz, Raquel Tejeda-Alejandre, Athena Zhang, Ciro A. Rodriguez 等 2024-09-06T15:01:26Z 置信度 0.70
• Effects of hydrogen-rich water on antioxidant activity during barley malting
  crossref Hao Zhu, Chunqiong Shi, Ying Xie, Guolin Cai 等 2024-03-01T10:02:23Z 置信度 0.70
• Decision matrix for integrating 3D printing technologies for biomanufacturing of alternative testing model
  crossref Jia Min Lee, Ming Jie Tan, Wai Cheung Ma, Wai Yee Yeong 2024-01-12T12:02:53Z 置信度 0.70
• Elucidating uptake and metabolic fate of dipeptides in CHO cell cultures using 13C labeling experiments and kinetic modeling
  crossref Harnish Mukesh Naik, Xiangchen Cai, Pranay Ladiwala, Jayanth Venkatarama Reddy 等 2024-03-07T16:42:48Z 置信度 0.70