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• An Experimentally Verified Mechanistic Model for Predicting Quorum Sensing-Based Switches.
  Quorum sensing-based genetic circuits are gaining traction in synthetic biology as they link population-level behaviour to individual cell responses. However, tuning these circuits remains challenging due to complex dynamics, particularly during the 'Learn' ph…
  pubmed De Baets J, De Mey M, De Paepe B 2026 Jul 置信度 0.82
• Integrated multi-omics analysis reveals a pH-driven metabolic and translational switch in Ureaplasma parvum.
  Human ureaplasmas are minimal-genome bacteria and pathobionts of the urogenital tract. They must adapt to fluctuating pH conditions despite the absence of canonical transcriptional regulatory systems. However, the mechanisms underlying these responses remain u…
  pubmed Hase H, Nakura Y, Shimada Y, Nishino A 等 2026 Jul 10 置信度 0.82
• Engineering 2,4-Diacetylphloroglucinol-Hyper-Tolerant Escherichia coli via Adaptive Evolution for Efficient Biosynthesis and an Organic Solvent-Free Downstream Process.
  2,4-Diacetylphloroglucinol (DAPG) is a valuable antimicrobial compound with significant agricultural potential, suffers bioproduction limitations from host toxicity and inefficient downstream processing. This study engineered DAPG-hyper-tolerant Escherichia co…
  pubmed Wang J, Liu M, Li C, Jiang B 等 2026 Jul 10 置信度 0.82
• In vivo spatial coordination with synthetic paracrine signaling.
  The immune system uses paracrine signaling to spatially confine potent responses such as inflammation. A bio-orthogonal synthetic paracrine system could enable engineering of analogous multicellular circuits in which different cell types coordinate their funct…
  pubmed Luo K, Ma Y, Li HR, Li H 等 2026 Jun 29 置信度 0.82
• Rewiring cellular immunotherapy: From CAR evolution to circuit innovations across diseases.
  Chimeric antigen receptor (CAR)-based immunotherapy has evolved from early linear receptor designs into increasingly sophisticated biological signal processing systems. While conventional CAR-T cell therapies have achieved remarkable clinical success, particul…
  pubmed Chen J, Cui Y, Du K, Liu J 等 2026 Jul 9 置信度 0.82
• Type 2 Inflammatory Diseases: The Crossroads of Immunity and Metabolism.
  The understanding of type 2 inflammatory diseases is undergoing a paradigm shift from "immune imbalance" toward "immune-metabolic crosstalk". Energy metabolism not only fuels immune responses but also fundamentally dictates the functional phenotypes of immune …
  pubmed Chen L, Pan ZX, Ma ZQ, Jin YX 等 2026 置信度 0.82
• Constructing an NF-κB Oscillator for Dual-Input Control in Yeast.
  Interactions between biological oscillators and environmental periodic signals are crucial for the correct regulation of cellular behaviors. Most synthetic biological oscillators only respond to one signal that initiates the oscillation, limiting the study of …
  pubmed Jiang Y, Fan Y, Zhang Z, Wei P 2026 置信度 0.82
• Coupled Perturbations of Gene Circuit Dynamics by Resource Competition and Growth Dilution.
  Resource competition and growth-mediated dilution are major sources of context dependence in synthetic gene circuits. However, their coupled effects on circuit behavior remain insufficiently dissected. This chapter presents a step-by-step experimental workflow…
  pubmed Youssef A, Zhang R, Tian XJ 2026 置信度 0.82
• Gene Expression Across Growth Stages (GEAGS): Modeling Circuit Dynamics in Batch Cultures.
  The dynamics of recombinant gene expression in bacterial systems are often modeled under the assumption of constant growth. However, most synthetic gene circuit assays are performed in enclosed batch cultures, where nutrient depletion and physiological transit…
  pubmed Hu CY, Namboothiri HR 2026 置信度 0.82
• Quantitative Coarse-Grained Modeling of Microbial Metabolism: Uncovering Growth Laws, Environmental Responses, and Host-Circuit Coupling.
  Microbial cell metabolism is highly complex, involving a vast number of molecular species and multi-level regulatory processes. To address this complexity and decode cellular behavior, coarse-grained modeling provides a powerful quantitative framework by group…
  pubmed Lai YH, Lu T 2026 置信度 0.82
• Modeling Growth-Mediated Dilution in Synthetic Circuits: Effects of Circuit Topology and Phase Separation.
  Growth-mediated dilution is a major source of variability and instability in synthetic gene circuits, influencing both expression dynamics and circuit memory. Our previous work demonstrated that circuit topology plays a critical role in determining how robustl…
  pubmed Tian XJ, Zhang R 2026 置信度 0.82
• From Negative Feedback to Integrated Control: Recombinase-Based Strategies for Mitigating Resource Competition.
  One of the key challenges in synthetic biology is the unintended interference between independently designed gene modules caused by resource competition within host cells. This interference disrupts circuit modularity and undermines the reliability of genetic …
  pubmed Zhang R, Zhang R, Tian XJ 2026 置信度 0.82
• Dynamic Robust Control of Microbial Communities Using Cybergenetics.
  A key enabler for the engineering of complex biological systems is the co-culturing different cell strains together in a microbial community. Co-cultures can exhibit more diverse capabilities, increased robustness, and greater efficiency than a single strain e…
  pubmed Lee TA, Stacey SB, Gallup O, Steel H 等 2026 置信度 0.82
• Feedback Control and Sensitivity Analysis in Synthetic Gene Circuits.
  Synthetic gene circuits are engineered DNA sequences that control when genes turn on and how much they are expressed, alongside the regulatory machinery of cells. Many gene circuits use feedback, such that the molecules produced by a gene increase or decrease …
  pubmed Baetica AA 2026 置信度 0.82
• Intercellular CRISPRi for Distributed Genetic Circuits.
  Microbial communities and multicellular organisms employ diverse strategies for allocation of available resources, achieved through task distribution among specialized cells. Drawing inspiration from nature, several synthetic multicellular circuits have been r…
  pubmed Pujar A, Sharma A, Kushwaha M 2026 置信度 0.82
• Programming RNA Trans-Splicing for Versatile Gene Regulation and Complex Cellular Logic Computation.
  Synthetic biology aims to customize biological systems by programming genetic circuits. However, the scale and complexity of genetic circuits are limited by a shortage of composable, programmable, and orthogonal regulatory parts. Recently, split-intron-enabled…
  pubmed Gao Y, Wang B 2026 置信度 0.82
• Cell-Free Genetic Circuits: Extract and Template Preparation.
  Cell-free systems have been highlighted as a transformative technology in synthetic biology, offering substantial technological advantages over traditional in vivo methods. By leveraging cellular metabolic machinery of the cells and decoupling cell growth from…
  pubmed Spartalis TR, Porche KB, Tang X, Kwon YC 2026 置信度 0.82
• Characterization of Synthetic Gene Circuits with Absolute Quantification in Continuous Culture.
  Despite rapid improvements in our ability to engineer novel biological systems, robust biodesign of synthetic gene circuits-networks of synthetic genes regulating each other-has been limited by a lack of standardized methods for understanding and reproducibly …
  pubmed Stacey SB, Lee TA, Gallup O, Csibra E 等 2026 置信度 0.82
• Characterizing Optogenetic Tools for Use in Synthetic Gene Circuits.
  Synthetic gene circuits consist of engineered networks of genes and proteins. The function of these circuits can be tuned by regulating the expression levels of key circuit components. Light is an ideal signal for controlling expression levels in these circuit…
  pubmed Wakiyama TH, McClean MN 2026 置信度 0.82
• Synthetic Biomolecular Condensates: Design Principles and Applications.
  Synthetic biomolecular condensates are engineered membraneless compartments formed through phase separation of biomacromolecules. Beyond lock-and-key interaction-dependent control of cellular processes, phase separation enables the spatiotemporal organization …
  pubmed Ma Y, Yang L, Dai Y 2026 置信度 0.82
• Design of Conditional Guide RNAs for the Logical Regulation of Gene Expression.
  The CRISPR interference (CRISPRi) is an RNA-guided regulator that silences gene expression by binding to its cognate DNA target, halting transcription in both prokaryotic and eukaryotic cells. Recent advances in RNA synthetic biology have endowed CRISPR guide …
  pubmed Park D, Shin W, Kang H, Kim J 2026 置信度 0.82
• Design of CRISPRi-Based Synthetic Gene Circuits in Bacteria.
  Synthetic gene circuits are key elements of engineered biological systems that allow us to control and program cellular behavior. Yet, circuit design can be challenging to newcomers due to the numerous design choices and the abundance and variety of parameters…
  pubmed Santos-Moreno J 2026 置信度 0.82
• Construction of a Tl-CRISPRi Genetic Circuit in Bacteria for Translation-Level Gene Knockdown.
  The Tl-CRISPRi system, which harnesses the specific RNA-binding activity of CRISPR-dCas13, has been recently developed for translation-level gene knockdown in bacteria. By introducing spacers complementary to the translation initiation region of the mRNA, dCas…
  pubmed Kim G, Kim HJ, Seo SW 2026 置信度 0.82
• A Golden Gate-Compatible CRISPR-Associated Transposon Tool for Multiplexed Bacterial Genome Editing.
  The insertion of large genetic circuits and metabolic pathways into bacterial genomes is becoming increasingly common within the field of synthetic biology due to the improved robustness and stability that come with genome integration. CRISPR-associated transp…
  pubmed Irvine TCT, Bailey AM, Gorochowski TE 2026 置信度 0.82
• Reimagining the Synthetic Biology DBTL Cycle with Machine Learning.
  While progress has been made in developing synthetic regulatory circuits for a variety of applications, circuit engineering remains highly unpredictable due to the inherent complexity of the intracellular medium. With recent advances in high-throughput molecul…
  pubmed Lee M, Wang Y, Rai K, Li S 等 2026 置信度 0.82
• Engineering commensal microbes for host health.
  Engineered live biotherapeutic products (eLBPs) represent an emerging class of programmable microbial therapies capable of sensing and responding to host physiology. Advances in microbiome science and synthetic biology have driven the development of engineered…
  pubmed Brown EA, Brevi A, Zong DM, Zarrinpar A 2026 Jul 8 置信度 0.82
• Duet between sugars and hormones: The molecular dialogue fine-tuning hypoxia acclimation in plants.
  Hypoxic conditions caused by submergence or soil waterlogging constrain plant growth and productivity. To survive, plants coordinately reprogram both sugar metabolism and phytohormone signaling to trigger adaptive responses; yet, the integrative regulatory fra…
  pubmed Ateeq M, Ashraf MA, Asim M, Huang X 等 2026 Jul 6 置信度 0.82
• Distinct roles of AdipoR1 and AdipoR2 in modulating neuronal firing homeostasis in response to adiponectin and AdipoRon.
  The regulation of neuronal network activity depends on interactions between intrinsic circuit mechanisms and metabolic signals. Adiponectin (APN) is a peripheral adipokine whose receptors are present in the central nervous system (CNS), but whether APN/APN rec…
  pubmed Jia X, Zheng C, Gong Q, Hu Y 等 2026 Jul 6 置信度 0.82
• Plant-Plant Communication for Systemic Acquired Resistance under Biotic Stress Spatiotemporally Tracked by an In Situ Surface-Enhanced Raman Spectroscopy Aerosol Spraying Analyzer.
  This study pioneers a surface-enhanced Raman spectroscopy (SERS) analyzer leveraging engineered Au core/Ag shell nanocubes (Au@AgNCs) to bridge in planta pathogen tracking with airborne defense signal monitoring, enabling unprecedented decoding of plant-plant …
  pubmed Li N, Song H, Ding Z, Qu C 等 2026 Jul 4 置信度 0.82
• Advancing synthetic biology with engineered chemically inducible gene regulatory systems.
  Chemically inducible gene regulatory systems, with inducible promoters as their core regulatory elements, are central to advancing synthetic biology by enabling precise, dynamic, and orthogonal control of gene expression in response to chemical signals. Recent…
  pubmed Li X, Su Y, Zhang Y, Su Y 等 2026 Jul 2 置信度 0.82
• Coordination of N(2)-fixing cell specialization and patterning in filamentous cyanobacteria by a uniquely structured σ factor.
  Cell differentiation and Turing-like patterning are tightly associated in a group of filamentous cyanobacteria that differentiate specialized N 2 -fixing cells, called heterocysts. Based on systematic genetic analyses, in particular genome-wide identification …
  pubmed Hu X, Liu X, Wang Y, Liu K 等 2026 Jul 7 置信度 0.82
• Combinatorial decision-making driven by multicomponent surface condensates.
  Living organisms rely on molecular networks, such as gene circuits and signaling pathways, for information processing and robust decision-making in crowded, noisy environments. Recent advances show that interacting biomolecules self-organize by phase transitio…
  pubmed Zentner A, Halingstad EV, Chalk C, Brenner MP 等 2026 Jul 7 置信度 0.82
• Targeting the Human Gut Microbiota-Between Conventional Therapy and Precision Genetic Engineering.
  The gut microbiota is increasingly examined as a therapeutic target because it contributes to epithelial barrier integrity, microbial metabolite production, bile acid transformation, immune regulation, and communication between the gut and distant organs. This…
  pubmed Ciurea NA, Mahdi L, Graziani A, Di Ciaula A 等 2026 Jun 17 置信度 0.82
• AI-driven cellular immunotherapy: transforming CAR-T engineering and translation.
  Chimeric antigen receptor T (CAR-T) cell therapy has demonstrated remarkable success in hematologic malignancies but faces persistent challenges in solid tumors and broader clinical translation, including limited target specificity, heterogeneous tumor antigen…
  pubmed Huang Z, Wang X, Lin L, Liu Y 等 2026 Jun 18 置信度 0.82
• [Research progress in inducible expression regulation systems in photosynthetic cyanobacteria].
  Cyanobacteria, as a group of prokaryotic photosynthetic microorganisms, have recently garnered widespread attention in the fields of synthetic biology and green biomanufacturing due to their ability to utilize light energy and carbon dioxide for autotrophic gr…
  pubmed Zhu L, Dong Z, Chen L, Sun T 等 2026 Jun 25 置信度 0.82
• Bioengineered bacteria in cancer immunotherapy: mechanistic advances, therapeutic strategies and clinical potential.
  Bioengineered bacteria have emerged as a transformative platform in cancer immunotherapy, offering unique opportunities for selective tumour targeting, immune modulation, and localised delivery of therapeutic molecules. Their natural ability to colonise hypoxi…
  pubmed Vadakkan K, Karippali S, Jacob J, Gunasekaran S 等 2026 Jun 24 置信度 0.82
• Synthetic biology of oncolytic bacteria: Comparative microbial chassis and precise killing strategies.
  The field of synthetic biology has become a revolutionary tool for engineering microorganisms capable of precise, programmed cancer treatment. Unlike conventional cancer treatments, which lack safe, selective toxicity, engineered microbial cells can detect tum…
  pubmed Hadid MA, Shaikhli NHA, Suhail OK, Hameed ST 等 2026 Dec 置信度 0.82
• Characterizing open-ended evolution through undecidability mechanisms in random Boolean networks.
  Discrete dynamical models underpin systems biology, but we still lack substrate-agnostic diagnostics for identifying finite-horizon dynamical signatures that may be relevant to open-ended evolution (OEE), such as the recurrent production of novel phenotypic st…
  pubmed López-Díaz AJ, Rivera Torres PJ, Febres GL, Gershenson C 2026 Jun 23 置信度 0.82
• Streptomyces: boost chassis strains for production of invaluable natural products.
  Streptomyces species continue to occupy a pivotal role in natural product biosynthesis, owing to their unparalleled metabolic diversity and genetic adaptability. This review focuses on cutting-edge advances documented from 2020 to 2025 (encompassing approximat…
  pubmed Chen S, Ling S, Li J, Liu Z 等 2026 Dec 置信度 0.82
• Engineered suppressor tRNAs enable precise translational control of genetic circuits in E. coli.
  Precise and orthogonal regulation of genetic circuits is a central challenge in synthetic biology, particularly at the translational level where tools remain scarce. Here, we address this by engineering suppressor transfer RNAs (sup-tRNAs) charged with canonic…
  pubmed Wang X, Xu J, Wang Y, Qi Q 等 2026 Jun 8 置信度 0.82
• Modular Scalable Synthetic Gene Circuits for Complex Functions Within Minimal Computational Layers in Human Cells.
  Engineering mammalian cells to execute complex genetic programs remains a significant challenge in synthetic biology. Synthetic gene circuits typically implement sophisticated programs through cascaded computational layers. However, these architectures require…
  pubmed Roas K, Kovalski I, Mouhadeb O, Aminov T 等 2026 Jun 16 置信度 0.82
• A Comprehensive Review on Metabolomics-Guided Metabolite Production in Plant Tissue Culture: Integrating Omics and Synthetic Biology for Enhanced Yields.
  The growing bioeconomy demands sustainable platforms for the production of high-value pharmaceuticals, nutraceuticals, and industrial compounds. Plant tissue culture (PTC) is an attractive alternative as it provides a means of controlled production of secondar…
  pubmed Manam M, Srivatsa KS, Mishra A, Paulraj Gundupalli M 2026 Jul 17 置信度 0.82
• Nucleotide-Level Chemical Reaction Network Modeling Enables Quantitative Prediction of Reconstituted Cell-Free Expression Systems.
  Cell-free expression systems offer a method for the rapid prototyping of DNA circuits and functional protein synthesis. While crude extracts remain a black box with many components carrying out unknown reactions, PURE contains only the required transcription a…
  pubmed Jurado Z, Pandey A, Murray RM 2026 Jul 17 置信度 0.82
• A red-emitting, genetically encoded indicator for two-photon voltage recording in vivo.
  Genetically encoded voltage indicators (GEVIs) enable minimally invasive, cell-type-specific optical measurements of neuronal membrane potential with millisecond temporal resolution. Red-shifted GEVIs are especially advantageous because they permit spectral mu…
  pubmed Yang S, McDonald AJ, Lu X, Villette V 等 2026 Jun 3 置信度 0.82
• The Promise of Synthetic Biology for Redesigning Plant Architecture.
  Ensuring global food security under accelerating climate change requires transformative approaches to crop improvement that extend beyond the limits of traditional breeding and gene editing. While domestication and modern agriculture have delivered substantial…
  pubmed Roychoudhry S, Dos Santos GD, Lloyd JPB 2026 May 28 置信度 0.82
• Gene expression in synthetic biology: Going with the light.
  Inducible expression of endogenous and foreign genes has been a pivotal driving force behind a lot many seminal breakthroughs in biotechnology. Synthetic biology, a very promising field, largely relies on transgene expression platforms which facilitate conveni…
  pubmed Pal P, Pal S, Mitra D 2026 Sep 置信度 0.82
• Enhancing small-molecule-mediated translational control through multivalent RNA aptamers.
  The precise translational control of gene expression by small molecules through RNA-based switches holds considerable promise for both research and therapeutic developments. However, current high-performance RNA switches remain limited in adaptability, with st…
  pubmed Feng HY, Wu XJ, Li XH, Liu Y 等 2026 Jun 8 置信度 0.82
• Cascading recombinase memory switch for programmable and stable gene expression in Pseudomonas putida.
  Genetic circuits for model bacteria often perform poorly in non-canonical hosts, limiting the deployment of regulatory programs in industrially relevant organisms. Here, we engineered an integrase-based cascading toggle switch that enables consistent and progr…
  pubmed Hernández-Sancho JM, Volke DC, Nikel PI 2026 Jun 8 置信度 0.82
• Engineering a PD-L1-sensing synthetic receptor for programmable macrophage response.
  Cellular decision-making relies on the integration of multiple extracellular cues into coordinated functional responses. Synthetic biology provides tools to rewire this process by engineering receptors that convert defined inputs into programmable outputs. Her…
  pubmed De Martino I, Russo L, Marchetti M, Siciliano V 2026 Jun 6 置信度 0.82
• Unsupervised deep learning enables blur-free resolution enhancement in two-photon microscopy.
  Two-photon microscopy enables the non-invasive imaging of deep living tissue. Quantitative three-dimensional analysis is hampered by axial blur and anisotropic resolution in two-photon microscopy. We introduce the two-photon microscopy image enhancement networ…
  pubmed Morita H, Hayashi S, Tsuji T, Kato D 等 2026 Jun 5 置信度 0.82
• Development strategies for engineered live biotherapeutic products for metabolic diseases.
  Metabolic diseases, such as obesity and diabetes, have risen due to lifestyle changes. Traditional treatments, including dietary modifications and pharmacological interventions, are limited by low compliance and adverse effects, highlighting the need for alter…
  pubmed Yun S, Choi KS, Min H, Jo YK 等 2026 Aug 置信度 0.82
• A novel reaction-diffusion architecture for engineering self-organized patterns in mammalian cells.
  Reaction-diffusion circuits generate self-organized spatial patterns through local activation and long-range inhibition, but synthetic implementations in mammalian cells have been limited by the differential-diffusion requirement. Here, we introduce a novel ar…
  pubmed Swedlund B, Danan JJ, Jiang TX, Tahar SB 等 2026 May 25 置信度 0.82
• Ratiometric transcriptional activation by protein degradation.
  Cells can respond to alterations in the abundances of specific proteins through transcriptional outputs. Synthetic approaches inspired by native post-transcriptional circuits that convert protein abundance changes into programmable gene expression would be tra…
  pubmed Gray MA, Randal KL, Co JA, Tang MT 等 2026 May 18 置信度 0.82
• Programmable, multiplexed and orthogonal gene control in bacteria with attenuated Cas13d systems.
  Cas13-based RNA effectors may enable dynamic, multiplexed and reversible gene regulation in bacteria. Yet, their widespread adoption is hindered by inherent cytotoxicity and collateral cleavage. Here we present a rational protein engineering strategy to genera…
  pubmed Tong S, Qin Y, Sun Y, Gao Y 等 2026 Jun 2 置信度 0.82
• Acetylcholine responsiveness of the BetI regulatory system in Escherichia coli.
  Acetylcholine (ACh) is widely recognized as a neurotransmitter in animals; however, its potential relevance to microbial physiology remains largely unexplored. Here, we report that the BetI regulatory system of Escherichia coli, previously characterized as a c…
  pubmed Satoh H, Harada K, Takemura Y, Suzuki S 等 2026 May 29 置信度 0.82
• Ligand-Induced Trigger RNA Cleavage Enables Programmable Gene Expression Regulation via Strand Displacement in Prokaryotic and Eukaryotic Cells.
  Synthetic RNA circuits offer powerful tools for reprogramming cellular behavior, but constructing ligand-responsive RNA switches that function reliably inside living cells remains challenging. Existing cis-acting designs often lack modularity and programmabili…
  pubmed Zhang C, Feng H, Li L, Lin J 等 2026 May 25 置信度 0.82
• Bioluminescent sentinel plants enable autonomous diagnostics of viral infections.
  Plants engineered with synthetic genetic programs can transform how we monitor and manage the extension of crop pests and diseases. Here, we establish a bioluminescent platform in Nicotiana benthamiana for autonomous viral sensing based on the fungal biolumine…
  pubmed Calvache C, Rodriguez-Rodriguez M, Vazquez-Vilriales V, Garcia-Perez E 等 2026 May 29 置信度 0.82
• Synthetic DNA circuits for programming cell functions.
  Synthetic biology aims to re-engineer living cells into autonomous computational chassis capable of executing sophisticated biological tasks. Within this framework, programmable nucleic acid-based logic networks have emerged as a versatile molecular control la…
  pubmed Wang M, Tang W, Jiang S, Wei P 2026 Aug 置信度 0.82
• A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications.
  T7 RNA polymerase is ubiquitously used in the fields of synthetic biology and biotechnology. Yet the ability to precisely and modularly regulate T7 RNAP remains surprisingly limited. Here, we engineer a T7 RNAP regulatory toolbox consisting of programmable syn…
  pubmed Lee PW, Mottaghi SS, Lugnier MG, Maerkl SJ 2026 May 28 置信度 0.82
• Self-driving medicine: closed-loop therapeutics for autonomous disease control.
  Closed-loop gene circuits are paving the way toward self-driving medicine: therapeutics that continuously sense physiological signals, compute responses, and autonomously tune therapeutic output. Yet clinical practice still relies largely on open-loop dosing a…
  pubmed Unal G, Fussenegger M 2026 May 28 置信度 0.82
• Synthetic gene circuits in tumor immunotherapy: design principles and applications.
  Tumor immunotherapy has revolutionized cancer treatment by harnessing the immune system to recognize and eliminate malignant cells. However, limited response rates, systemic toxicity, and tumor heterogeneity remain major challenges. Synthetic gene circuits - e…
  pubmed Tian J, Wang Z, Ye H 2026 Aug 置信度 0.82
• Engineered Optogenetic Circuits In Yeast with Self-Sustained Outputs.
  Optoswitches are of particular interest to the metabolic engineering community, as light has a superior advantage of tunability and reversibility. However, the light-shading effect at industrial scales remains an unsolved challenge. Here, we report optogenetic…
  pubmed Fan C, Chen H, Wang Y, Wang J 等 2026 May 27 置信度 0.82
• Synthetic biology-driven bioinspired delivery systems for RNA therapeutics in neural repair.
  RNA therapeutics offer transformative potential for neural repair. However, various delivery challenges continue to hinder the clinical translation of RNA therapeutics. Synthetic biology, as an interdisciplinary cutting-edge field, improves delivery systems by…
  pubmed Shen J, Qu C, Wang Y, Lu Z 等 2026 May 14 置信度 0.82
• Classical Phytohormones and Peptide Plant Hormones in Abiotic Stress Tolerance: Crosstalk, Physiological Integration, and Crop Improvement.
  Plants are constantly exposed to a wide range of abiotic stresses that have significant negative impacts on growth and yield. Plant acclimation to these stresses is governed by integrated classical phytohormone and plant peptide hormone signalling networks tha…
  pubmed Ali B, Imran A, Iftikhar H, Khan Z 等 2026 May 18 置信度 0.82
• Current Progress and Future Outlook for Synthetic Gene Circuits in Cardiovascular Therapy.
  Despite decades of therapeutic advances, cardiovascular diseases remain the leading cause of global mortality, underscoring the need for strategies that move beyond untargeted systemic pharmacotherapy. Synthetic biology introduces a programmable therapeutic pa…
  pubmed Khalilitousi M, Dhingra A, Rohani L, Weiss R 2026 May 21 置信度 0.82
• Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.
  Engineering CRISPR-Cas systems for improved or altered function is critical to both research and therapeutic applications. Unfortunately, most optimization, especially directed evolution in bacterial hosts, fails to capture the functional requirements of the c…
  pubmed Sabol AL, Mengiste AA, Singh P, Sreekanth V 等 2026 Jun 2 置信度 0.82
• Engineering quorum sensing: new directions for antimicrobial therapy.
  Quorum sensing (QS) is a cell-cell communication mechanism that enables bacteria to coordinate gene expression in response to population density and community composition. In many pathogens, QS plays a central role in host colonization and virulence, making it…
  pubmed Urbauer E, Gräwe A, Chamas A, Papenfort K 2026 May 26 置信度 0.82
• Synthetic Biology for Next-Generation Lactic Acid Bacteria: Engineering the Future of Food.
  Lactic acid bacteria (LAB), as one of the key microorganisms in the food industry, play a crucial role in functional food fermentation. However, current LAB strains are limited by challenges, such as plasmid instability, low gene expression efficiency, and com…
  pubmed Li M, Naeem M, Guo Z, Dong T 等 2026 Jun 3 置信度 0.82
• Prospects and challenges in using engineered lactic acid bacteria in aquaculture applications.
  Despite the considerable potential of lactic acid bacteria (LAB) as probiotics, there is a fundamental gap between the functional limitations of wild-type strains and the complex demands of aquaculture. Modular and intelligent engineering strategies are the pr…
  pubmed Huang X, Sun X, Dong X, Tang Y 等 2026 Jun 置信度 0.82
• Integrating Cells, Biomaterials, and Advanced Engineering for Next-Generation Peripheral Nerve Repair.
  Peripheral nerve injuries (PNIs) disrupt sensory and motor circuits, affecting millions worldwide and often resulting in lasting functional deficits. Autologous nerve grafting remains the surgical benchmark for segmental defects, yet limited graft length, dono…
  pubmed Galahad Declercq GL, Song S 2026 May 22 置信度 0.82
• Stimulus-responsive engineered oncolytic bacteria.
  Oncolytic bacteria have emerged as a promising platform for targeted cancer therapy owing to their intrinsic ability to preferentially colonize tumor tissues, induce direct tumor cell killing, and remodel the tumor microenvironment to activate antitumor immuni…
  pubmed Chen J, Liu Y, Wu Y, Liu Z 2026 Nov 置信度 0.82
• Dynamics of inducible genetic circuits.
  Genes are connected in complex networks of interactions where often the product of one gene is a transcription factor that alters the expression of another. Many of these networks are based on a few fundamental motifs leading to switches and oscillators of var…
  pubmed Yang Z, Rousseau RJ, Mahdavi SD, Garcia HG 等 2026 Apr 置信度 0.82
• Toward predictable and programmable genetic circuits in plants.
  Predictable design is central to realizing the potential of plant genetic circuits by linking regulatory architecture to phenotypic outcomes. Despite rapid advances in genetic parts and circuit construction, most plant circuits are still developed through empi…
  pubmed Kong C, Zhang J 2026 Sep 置信度 0.82
• Micropeptides in Cancer and Immunity: Diagnostic, Therapeutic, and Synthetic Biology Frontiers.
  Micropeptides are emerging as a previously hidden layer of the human proteome, redefining the long-standing separation between coding and noncoding genomic regions. Once dismissed as translational noise, sORFs embedded within lncRNAs, circRNAs, pseudogenes, an…
  pubmed Majzoub RE, Wehbe R, Said HE, Fneish A 等 2026 Aug 20 置信度 0.82
• Modular Assembly of Higher-Order DNA Nanotube Tile Nanostructures Using DNA Annular Scaffolds.
  Inspired by natural systems, synthetic biology employs diverse biomaterials to construct artificial systems on the basis of the principle of modular assembly. However, the rational construction of predictable, stable, and logically controllable higher-order na…
  pubmed Zhi S, Wang J, Ren S, Wang L 等 2026 Jun 3 置信度 0.82
• Synthesis of Programmable Bioelectronic Yeast for Biohybrid Futures.
  The budding yeast Saccharomyces cerevisiae has moved beyond an evolved and domesticated single-celled microorganism into a deliberately engineered biological substrate. Advances in synthetic genomics, illustrated by the international Synthetic Yeast Genome (Sc…
  pubmed Erpf PE, Kinder BD, Archer E, Walker RSK 等 2026 Jan-Dec 置信度 0.82
• Reprogramming endogenous NK circuits by highly efficient nonviral genome editing.
  Natural killer (NK) cells are promising platforms for off-the-shelf immunotherapy, yet nonviral precision engineering remains limited by poor HDR efficiency, DNA toxicity, and manufacturing challenges. The aim of this study was to establish a high-yield, nonvi…
  pubmed Huang RS, Phung SK, Sumstad D, Weis AJ 等 2026 Jul 6 置信度 0.82
• EL222-Based Optogenetic Gene Regulation in Methylotrophic Yeasts: Mechanisms, Applications, and Future Directions.
  Methylotrophic yeasts such as Pichia pastoris are widely used for heterologous protein production because they contain strong and tightly regulated promoters. However, the use of methanol as an inducer presents several practical challenges, including toxicity,…
  pubmed Joon R, Phutela R, Khare P 2026 Jul 置信度 0.82
• Decoding Early Neurochemical Dynamics in Circuit Dysfunction of Parkinson's Disease via Synergetic SERS and Electrophysiological Probe Suite.
  The pathological mechanisms underlying neurodegenerative diseases remain poorly defined, largely due to the lack of tools capable of detecting molecular alterations before the onset of clinical symptoms. Here we successfully achieve targeted profiling of dopam…
  pubmed Yin Y, Zhou B, Zhang W, Feng Y 等 2026 May 26 置信度 0.82
• Quantitative Measurement of Synthetic Repression Curves Reveals Design Challenges for Genetic Circuit Engineering under Growth Arrest.
  Despite the fact that microbes in natural environments spend most of their time in growth arrest, we understand little about how this physiological state affects the performance of engineered genetic circuits. Here, we measure repression curves from a library …
  pubmed Marken JP, Prator ML, Hay BA, Murray RM 2026 May 13 置信度 0.82
• The Synthetic Cannabinoid AKB-48 Induces Cell Death in Murine Cerebellum Through Different Signaling Pathways.
  Novel psychoactive substances (NPSs) exhibit extremely strong pharmaco-toxicological activity, often leading to severe adverse effects that pose a serious risk to consumers' health. Among these, synthetic cannabinoids (SCs) currently represent the majority of …
  pubmed De Luca F, Corli G, Bassi M, Bilel S 等 2026 Apr 27 置信度 0.82
• Synthetic Biology: Integrated Gene Circuits
  A major goal of synthetic biology is to develop a deeper understanding of biological design principles from the bottom up, by building circuits and studying their behavior in cells. Investigators initially sought to design circuits "from scratch" that function…
  openalex Nagarajan Nandagopal, Michael B. Elowitz 2011-09-01 置信度 0.72 Synthetic biologyElectronic circuitNeuronal circuitsComputer scienceBiology
• Synthetic biology: understanding biological design from synthetic circuits
  openalex Shankar Mukherji, Alexander van Oudenaarden 2009-11-09 置信度 0.72 Synthetic biologyBiologyGRASPRational designSystems biology
• Designing cell function: assembly of synthetic gene circuits for cell biology applications
  openalex Mingqi Xie, Martin Fussenegger 2018-06-01 置信度 0.72 Synthetic biologyBiomedicineRegenerative medicineComputational biologyFunction (biology)
• Synthetic biology: applications come of age
  openalex Ahmad S. Khalil, James J. Collins 2010-04-16 置信度 0.72 Synthetic biologyBiologyComputational biologySystems biology
• Quantitative characterization of genetic parts and circuits for plant synthetic biology
  openalex Katherine A. Schaumberg, Mauricio S. Antunes, Tessema Kassaw, Wenlong Xu 等 2015-11-16 置信度 0.72 Synthetic biologyProtoplastBiological systemComputational biologyNormalization (sociology)
• From molecular to modular cell biology
  openalex Leland H. Hartwell, J. J. Hopfield, Stanislas Leibler, Andrew W. Murray 1999-12-02 置信度 0.72 Modular designComputational biologySynthetic biologySystems biologyBiology
• Synthetic Biology: A Unifying View and Review Using Analog Circuits
  We review the field of synthetic biology from an analog circuits and analog computation perspective, focusing on circuits that have been built in living cells. This perspective is well suited to pictorially, symbolically, and quantitatively representing the no…
  openalex Jonathan Jin Yuan. Teo, Sung Sik Woo, Rahul Sarpeshkar 2015-08-01 置信度 0.72 Electronic circuitAnalogue electronicsComputer scienceElectronic engineeringElectrical engineering
• Synthetic biology
  openalex Steven A. Benner, A. Michael Sismour 2005-07-01 置信度 0.72 Synthetic biologyBiologyNatural (archaeology)Function (biology)Computational biology
• Butyrolactone signalling circuits for synthetic biology
  Signalling circuits based on quorum sensing mechanisms have been popular tools for synthetic biology. Recent advances in our understanding of the analogous systems regulating antibiotics production in soil bacteria suggest that these might provide useful compl…
  openalex Marc Biarnes-Carrera, Rainer Breitling, Eriko Takano 2015-07-09 置信度 0.72 Synthetic biologyElectronic circuitSignallingQuorum sensingComputer science
• Biomedically relevant circuit‐design strategies in mammalian synthetic biology
  The development and progress in synthetic biology has been remarkable. Although still in its infancy, synthetic biology has achieved much during the past decade. Improvements in genetic circuit design have increased the potential for clinical applicability of …
  openalex William I. Bacchus, Dominique Aubel, Martin Fussenegger 2013-09-24 置信度 0.72 Synthetic biologyBiologyMulticellular organismComputational biologySystems biology
• Wearable materials with embedded synthetic biology sensors for biomolecule detection
  Integrating synthetic biology into wearables could expand opportunities for noninvasive monitoring of physiological status, disease states and exposure to pathogens or toxins. However, the operation of synthetic circuits generally requires the presence of livi…
  openalex Peter Q. Nguyen, Luis R. Soenksen, Nina M. Donghia, Nicolaas M. Angenent-Mari 等 2021-06-28 置信度 0.72 Wearable computerCRISPRSynthetic biologyNanotechnologyNucleic acid
• Synthetic DNA Synthesis and Assembly: Putting the Synthetic in Synthetic Biology
  The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes, circuits, and even entire genomes by gene synthesis methods has become an enabling technology for modern molecular biology and enables the design, build, test, learn, and r…
  openalex Randall A. Hughes, Andrew D. Ellington 2017-01-01 置信度 0.72 Synthetic biologyBiologyOligonucleotideDNA synthesisComputational biology
• Synthetic Biology Moving into the Clinic
  Synthetic biology is an emerging field focused on engineering biomolecular systems and cellular capabilities for a variety of applications. Substantial progress began a little over a decade ago with the creation of synthetic gene networks inspired by electrica…
  openalex Warren C. Ruder, Ting Lu, James J. Collins 2011-09-01 置信度 0.72 Synthetic biologyVariety (cybernetics)Regenerative medicineSystems biologyBiology
• Designing Biological Circuits: Synthetic Biology Within the Operon Model and Beyond
  In 1961, Jacob and Monod proposed the operon model of gene regulation. At the model's core was the modular assembly of regulators, operators, and structural genes. To illustrate the composability of these elements, Jacob and Monod linked phenotypic diversity t…
  openalex Max A. English, Raphaël V. Gayet, James J. Collins 2021-03-30 置信度 0.72 Synthetic biologyComputational biologyOperonBiologygal operon
• Materials design by synthetic biology
  openalex Tzu‐Chieh Tang, Bolin An, Yuan‐Yuan Huang, Sangita Vasikaran 等 2020-12-23 置信度 0.72 Synthetic biologyLiving systemsNanotechnologyComputer scienceBiomimetics
• Paper-Based Synthetic Gene Networks
  openalex Keith Pardee, Alexander A. Green, Tom Ferrante, D. Ewen Cameron 等 2014-10-24 置信度 0.72 BiologyComputational biologyGeneGeneticsGene regulatory network
• Synthetic biology to access and expand nature's chemical diversity
  openalex Michael J. Smanski, Hui Zhou, Jan Claesen, Ben Shen 等 2016-02-15 置信度 0.72 Synthetic biologyBiologyComputational biologyGenomeENCODE
• High-Performance Biocomputing in Synthetic Biology–Integrated Transcriptional and Metabolic Circuits
  Biocomputing uses molecular biology parts as the hardware to implement computational devices. By following pre-defined rules, often hard-coded into biological systems, these devices are able to process inputs and return outputs─thus computing information. Key …
  openalex Ángel Goñi‐Moreno, Pablo I. Nikel 2019-03-11 置信度 0.72 Synthetic biologyComputer scienceMetabolic engineeringConstruct (python library)Conceptualization
• A fast, robust and tunable synthetic gene oscillator
  openalex Jesse Stricker, Scott Cookson, Matthew R. Bennett, William Mather 等 2008-10-29 置信度 0.72 Synthetic biologyGene regulatory networkComputer sciencePopulationRational design