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Few advances have effectively addressed the challenge of selectively activating aspartic acid (Asp) side chains. The β-carboxyl group of Asp remains underutilized as a reactive handle due to its limited reactivity under biocompatible conditions, its susc…
pubmed
Lei Y, Zhao K, Guo M, Weis TA 等
2026 Aug 19
置信度 0.82
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In targeted protein degradation (TPD), specific subcellular proteins are removed by routing them to the ubiquitin-proteasome, autophagy, or lysosome machinery. For instance, proteolysis-targeting chimeras (PROTACs) are synthetic heterobifunctional small molecu…
pubmed
Mylemans B, Korona B, Acevedo-Jake AM, MacRae A 等
2026 Aug 19
置信度 0.82
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With global warming, many species will shift their geographic ranges coldward (i.e., towards higher latitudes and elevations) to remain within suitable thermal conditions. At the community level, these range shifts result in "thermophilization"-the increasing …
pubmed
Feeley KJ, Iseli E, Bektaş B, Manzanedo RD 等
2026 Aug
置信度 0.82
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The validation of methods is an integral part of statistical research, defining conditions under which methods yield reliable results. Empirical validation requires a solid data basis to control and manage relevant characteristics like sample size, dimensional…
pubmed
Marchi H, Schmiegel S, Schamberger T, Fuchs C
2026
置信度 0.82
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The limitations of DNA synthesis technologies are a fundamental bottleneck in synthetic biology. Here we present a high-throughput gene synthesis method driven by hybridization, called Molecular Self-Assembly Induced Cloning. Molecular Self-Assembly Induced Cl…
pubmed
Wu Z, Sun Z, Jiang S, Wang Y 等
2026
置信度 0.82
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The multienzyme cascade conversion of CO 2 to methanol is a promising route for sustainable chemical synthesis, yet the reduction of formate to formaldehyde remains its major bottleneck. Here, we use quantum chemical calculations to elucidate the catalytic mec…
pubmed
Liu B, Jiang Y, Su H, Sheng X
2026 Aug
置信度 0.82
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Precise manipulation of large DNA fragments in eukaryotic genomes remains limited by the low efficiency and delivery constraints of current multicomponent editing systems. In this study, we engineered an RNA-guided bridge recombinase system through rational mu…
pubmed
Gao R, Wei J, Sun C, Liu J 等
2026 Aug 19
置信度 0.82
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Programmable biofunctionalization of bacterial cellulose (BC) is promising for constructing engineered living materials, but current microbial co-culture approaches struggle to combine coherent matrix formation, dynamic cultivation, and efficient matrix-associ…
pubmed
Liu F, Wang Y, Cui B, Feng Y 等
2026
置信度 0.82
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Electronic waste (E-waste) is one of the fastest growing waste streams worldwide, posing serious challenges for environmental and human health, while representing a rich secondary source of valuable metals. Among these technology-critical metals (TCMs) are tho…
pubmed
Mou TJ, Horsfall LE
2026
置信度 0.82
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Asparagine (Asn) is pivotal for plant nitrogen (N) metabolism and plays indispensable roles in plant growth, development, and stress tolerance. However, the systematic characteristics and core functions of asparagine synthetase genes (NtASNs) in tobacco remain…
pubmed
Zhang YQ, Huang XY, Xi WX, Yang PS 等
2026
置信度 0.82
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Breast cancer remains a major health concern among women, characterized by a high risk and substantial mortality. Chemotherapy is widely employed as a standard treatment modality to eliminate malignant cells and improve patient survival. Nevertheless, recurren…
pubmed
Sun C, Lv L, Xu Q, Wu S 等
2026 Aug 19
置信度 0.82
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Shigella is the leading cause of morbidity and mortality in children younger than 5 years in low- and middle-income countries and is a major actor in antimicrobial resistance. An effective vaccine is urgently needed. SF2a-TT15, a conjugate featuring a syntheti…
pubmed
Sawe F, Koech L, Neffati S, Korir B 等
2026 Aug 19
置信度 0.82
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Fungi transition between cell morphologies to adapt to and colonize environments. For Candida albicans, commensal colonization of humans and virulence depend on a reversible switch between invasive hyphae and disseminating yeast cells. The yeast-to-hyphae tran…
pubmed
Bharathwaj M, Hofferek V, Swaminathan A, Turner R 等
2026 Aug 19
置信度 0.82
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Embryonic development builds tissues and organs through the self-organization of heterogeneous cells across spatial and temporal scales, analogous to an ecosystem. Although ecological systems are often stochastic and multistable, embryogenesis is remarkably re…
pubmed
Keshavarz K, Ebrahimkhani MR
2026
置信度 0.82
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Human protein-coding genes evolved via rearrangement of domains from ancestral genes. We develop a scalable, evolutionarily guided method to assemble novel genes from constituent domains within a protein family, termed DESynR (domain engineered via synthesis a…
pubmed
Takacsi-Nagy O, Kasinathan S, Hartman A, Yin Y 等
2026 Aug 19
置信度 0.82
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A novel series of thiazolidinone derivatives (3a-3h) were synthesized through the condensation of 2-(arylamino)-thiazol-4-one and pyrrole-3-carbaldehyde utilizing glacial acetic acid as a medium and physical characterization was done by IR, 1 H NMR and MS tech…
pubmed
DkharGatphoh BF, Ramesh A, Prabhakar PS, Kumar M 等
2026 Aug 12
置信度 0.82
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In a process known as phenotypic heterogeneity or phenotypic variation, bacteria can produce distinct phenotypes within an isogenic population in response to shifting environmental conditions. Noise in gene expression, asymmetric cell division, phase variation…
pubmed
Cámara-Almirón J, Jaskólska M, Veening JW
2026
置信度 0.82
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Direct hydrogenation of carbon dioxide (CO 2 ) to acetic acid, a key commodity chemical, offers a sustainable route to valorize greenhouse gases but is plagued by CO 2 inertness, thermodynamic barriers, and poor selectivity for C-C coupling over competing over…
pubmed
Yang C, Wu B, Xu H, Hu D 等
2026 Aug 21
置信度 0.82
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The consequence of enhanced energy metabolism for health and life span of organisms is a fundamental yet controversially discussed issue of life and aging among others because of the fact that increasing energy metabolism often entails the risk of oxidative da…
pubmed
Liu Y, Hu Z, Duan D, Wu L 等
2026 Aug 21
置信度 0.82
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CRISPR-Cas13d is increasingly used for RNA knockdowns, but off-target cleavage of near-cognate RNAs hinders its broader adoption. Here, we solve seven cryo-electron microscopy structures of wild-type Cas13d in complex with matched and mismatched targets. These…
pubmed
Chou CW, Sinan S, Kuo HC, Chang YC 等
2026 Aug 21
置信度 0.82
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Understanding how osteoblasts build and remodel bone matrix in vivo remains a fundamental challenge because cellular metabolism and matrix turnover are difficult to resolve across time and space within mineralized tissues. Here, we developed an integrated imag…
pubmed
Chen K, Guo J, Chen X, Chen B 等
2026 Aug 25
置信度 0.82
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Protozoan parasites of the genus Leishmania, the causative agents of human leishmaniasis, have evolved mechanisms to manipulate host cell functions to their advantage. Recently, small non-coding RNAs have been identified as contributors to the pathogenesis of …
pubmed
Brar HK, Moradimotlagh A, Longowal DK, Moon KM 等
2026
置信度 0.82
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Nowadays, enzyme engineering has moved from traditional structure-based mutagenesis and directed evolution to data-intensive, AI-assisted design paradigms that involve the rapid discovery and optimization of biocatalysts. Whereas classical approaches relied on…
pubmed
Ullah M, Rizwan M, Andoh V, Raza A 等
2026 Aug 19
置信度 0.82
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Selenium deficiency poses a global health concern, yet traditional supplements face limitations in bioavailability and safety. Probiotics-derived selenium nanoparticles (SeNPs) offer a promising alternative. This review summarizes the biosynthesis mechanisms o…
pubmed
Sheng X, Deng X, Yang C, Xiang Q 等
2026
置信度 0.82
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This chapter examines the concept of ownership and intellectual property in synthetic biology, revealing how ownership models act as governance tools by empowering and controlling certain actors, thus shaping scientific and commercial trajectories. It outlines…
crossref
Emma Frow, Robert Smith, Lalitha Sundaram
2026-08-04T16:44:41Z
置信度 0.70
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This chapter outlines the meaning and role of governance in synthetic biology, highlighting the diverse ways societies can shape and steer technology development. It emphasises that synthetic biology does not evolve in isolation but emerges within a complex so…
crossref
Emma Frow, Robert Smith, Lalitha Sundaram
2026-08-04T16:44:40Z
置信度 0.70
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Since Nirenberg and Matthaei used cell-free protein synthesis (CFPS) to elucidate the genetic code in the early 1960s [...]
crossref
Seok Hoon Hong
2019-10-09T03:14:33Z
置信度 0.70
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crossref
Kevin Smith
2022-01-14T22:43:51Z
置信度 0.70
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crossref
Deepak Singh Pawan K Dhar
2013-12-20T04:55:13Z
置信度 0.70
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crossref
2016-09-08T14:55:01Z
置信度 0.70
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crossref
Jyoti Singh, Aditi Sharma, Anupam Jyoti, Lalit Sharma
2026-05-18T00:39:43Z
置信度 0.70
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crossref
Umesh Panwar, Poonam Singh, Sanjeev Kumar Singh
2018-10-01T16:24:30Z
置信度 0.70
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crossref
Arnaz Ranji, Jeffrey C. Wu, Bradley C. Bundy, Michael C. Jewett
2013-09-09T16:22:33Z
置信度 0.70
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This chapter examines the relationship between funding and governance in synthetic biology, showing how funding organisations actively shape both research priorities and the development of the field. It outlines how public research councils, government agencie…
crossref
Emma Frow, Robert Smith, Lalitha Sundaram
2026-08-04T16:44:41Z
置信度 0.70
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crossref
Sergio Spaans, Noortje A. M. Bax, Carlijn V. C. Bouten, Patricia Y. W. Dankers
2017-12-18T10:37:25Z
置信度 0.70
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crossref
Tobias Eichinger
2015-11-26T13:46:42Z
置信度 0.70
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crossref
Xianhao Xu, Yanfeng Liu, Guocheng Du, Long Liu
2020-07-24T07:19:21Z
置信度 0.70
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crossref
Tim Weenink, Tom Ellis
2013-08-30T20:48:21Z
置信度 0.70
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crossref
Manuel Porcar, Juli Peretό
2014-09-09T02:25:16Z
置信度 0.70
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crossref
Manuel Porcar, Juli Peretó
2014-09-09T02:25:16Z
置信度 0.70
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crossref
Manuel Porcar, Juli Peretó
2014-09-09T02:25:16Z
置信度 0.70
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crossref
Julia Rijssenbeek
2025-07-03T08:15:08Z
置信度 0.70
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crossref
Sarangam Majumdar, Sisir Roy
2020-09-15T11:03:35Z
置信度 0.70
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Abstract Self-regeneration is a fundamental function of all living systems. Here we demonstrate molecular self-regeneration in a synthetic cell model. By implementing a minimal transcription-translation system within microfluidic reactors, the system was able …
crossref
Barbora Lavickova, Nadanai Laohakunakorn, Sebastian J. Maerkl
2020-07-04T11:25:12Z
置信度 0.70
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crossref
Marnix H. Medema, Rainer Breitling, Eriko Takano
2012-05-17T14:16:52Z
置信度 0.70
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Abstract Synthetic biology has created oscillators, latches, logic gates, logarithmically linear circuits, and load drivers that have electronic analogs in living cells. The ubiquitous operational amplifier, which allows circuits to operate robustly and precis…
crossref
Ji Zeng, Jaewook Kim, Areen Banerjee, Rahul Sarpeshkar
2017-07-11T11:35:23Z
置信度 0.70
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crossref
2020-01-30T09:32:06Z
置信度 0.70
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crossref
2013-09-09T20:22:30Z
置信度 0.70
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crossref
Ehtisham Ul Haq, Muneeb Ahmad Khan, Kinza Mujahid, Mustafeez Mujtaba Babar
2026-05-17T22:46:23Z
置信度 0.70
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crossref
Michael Bölker
2014-09-27T04:08:50Z
置信度 0.70
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crossref
Rachel Armstrong, Markus Schmidt, Mark Bedau
2012-08-03T19:45:49Z
置信度 0.70
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crossref
Gan Mudan, Chen Chao
2026-01-12T06:16:09Z
置信度 0.70
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crossref
Amir Pandi, Olivier Borkowski, Jean-Loup Faulon
2020-04-13T11:02:57Z
置信度 0.70
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crossref
Alfred Nordmann
2014-09-27T04:08:50Z
置信度 0.70
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crossref
Raz Jelinek
2018-04-13T02:12:09Z
置信度 0.70
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crossref
Ilaria de Lisa
2020-09-28T22:02:30Z
置信度 0.70
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crossref
Sylvia M. Clay, Stephen S. Fong
2012-09-24T14:48:25Z
置信度 0.70
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crossref
Beth Baker
2024-01-30T10:33:10Z
置信度 0.70
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crossref
Patrick Smadbeck, Yiannis N. Kaznessis
2014-12-08T14:39:11Z
置信度 0.70
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crossref
Manuel Porcar, Juli Peretó
2014-09-09T02:25:16Z
置信度 0.70
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crossref
Mohamed Helmy, Kumar Selvarajoo
2022-10-13T08:04:43Z
置信度 0.70
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crossref
Amelie Cserer, Alexandra Seiringer
2009-10-08T22:50:37Z
置信度 0.70
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crossref
2009-09-22T20:46:30Z
置信度 0.70
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crossref
2016-12-03T21:32:52Z
置信度 0.70
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crossref
Aidan Hollis
2013-07-16T10:02:55Z
置信度 0.70
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Science is not an isolated enterprise: it is affected by and affects broader society and culture. Synthetic biology has changed aspects of education; stimulated artists, writers, and film-makers; and caught the interest of philosophers, ethicists, campaigners,…
crossref
Jamie A. Davies
2018-07-26T06:19:31Z
置信度 0.70
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Synthetic biology has ‘two souls’, one focused on engineering new features into existing life, the other aiming to create life de novo . The goal of creating life is arguably far more significant in terms of long-term scientific and philosophical impact. ‘Crea…
crossref
Jamie A. Davies
2018-07-26T06:20:13Z
置信度 0.70
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crossref
Tom Ellis, Yizhi Cai
2016-10-21T04:48:59Z
置信度 0.70
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crossref
Pachara Sattayawat, Wasu Pathom-aree
2022-01-29T10:02:47Z
置信度 0.70
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crossref
Hue Vu Thi, Minh-Hanh Nguyen, Mai-Trang Ngo, Dinh-Toi Chu
2026-05-17T22:38:40Z
置信度 0.70
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crossref
Michael C. Jewett
2014-06-20T04:04:56Z
置信度 0.70
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crossref
Naga Bhushana Rao Karampudi
2025-11-08T11:00:56Z
置信度 0.70
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Abstract Reproducibility has been identified as an outstanding challenge in science, and the field of synthetic biology is no exception. Meeting this challenge is critical to allow the transformative technological capabilities emerging from this field to reach…
crossref
Matthew W Lux, Elizabeth A Strychalski, Gary J Vora
2023-11-12T14:19:28Z
置信度 0.70
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crossref
Ashty S. Karim, Michael C. Jewett
2018-06-27T22:42:45Z
置信度 0.70
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crossref
Sarangam Majumdar, Sisir Roy
2020-09-15T11:03:35Z
置信度 0.70
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crossref
Ilaria de Lisa
2020-09-28T22:02:30Z
置信度 0.70
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crossref
Kevin Munnelly
2013-05-07T12:44:33Z
置信度 0.70
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Abstract Genotype networks are sets of genotypes connected by small mutational changes that share the same phenotype. They facilitate evolutionary innovation by enabling the exploration of different neighborhoods in genotype space. Genotype networks, first sug…
crossref
Javier Santos-Moreno, Eve Tasiudi, Hadiastri Kusumawardhani, Joerg Stelling 等
2022-09-02T10:50:15Z
置信度 0.70
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crossref
Guo-Qiang Chen
2012-07-20T04:01:47Z
置信度 0.70
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crossref
Seok Hoon Hong, Ana Serratosa Fernandez-Baca
2022-01-14T22:46:15Z
置信度 0.70
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crossref
Srinivasan Ganesh Kumar, Ashok Ganesan, Shashi Kumar
2022-01-14T22:42:49Z
置信度 0.70
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Abstract Viruses infecting humans are manifold and several of them provoke significant morbidity and mortality. Simulations creating large synthetic datasets from observed multiple viral strain infections in a limited population sample can be a powerful tool t…
crossref
Ville N. Pimenoff, Ramon Cleries
2021-07-14T12:55:16Z
置信度 0.70
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Abstract Real-time azide or azido-functionalized molecular detection inside living cells using bioorthogonal chemistry-based approaches has been revolutionary to advancing chemical-biology. These methods have enabled diverse applications ranging from understan…
crossref
Chandra Kanth Bandi, Kyle S. Skalenko, Ayushi Agrawal, Neelan Sivaneri 等
2020-07-10T05:45:23Z
置信度 0.70
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crossref
Andrew Lustig
2014-01-27T21:17:46Z
置信度 0.70
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crossref
Bijan Zakeri, Timothy K. Lu
2012-11-19T19:58:28Z
置信度 0.70
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crossref
Assaf A. Gilad, Mikhail G. Shapiro
2017-02-17T09:28:21Z
置信度 0.70
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crossref
2013-09-09T20:22:49Z
置信度 0.70
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crossref
2025-07-08T09:32:46Z
置信度 0.70
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This chapter focuses on the application of synthetic biology to biotechnology. Despite being a poorly defined field, synthetic biology is a technology with the potential to transform microbial biotechnology as it allows for the development of organisms that ca…
crossref
Kay Yeoman, Beatrix Fahnert, David Lea-Smith, Tom Clarke
2024-08-05T17:39:26Z
置信度 0.70
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crossref
Gerd Klöck
2014-09-27T04:08:50Z
置信度 0.70
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crossref
Huimin Zhao
2022-11-18T08:25:42Z
置信度 0.70
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crossref
Georg Toepfer
2016-04-15T09:10:13Z
置信度 0.70
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This chapter contains sections titled: Introduction Biological Oscillators Cell–Cell Communication in Bacteria Conclusion References
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Wilson W. Wong, James C. Liao
2009-09-22T20:46:30Z
置信度 0.70
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crossref
Alexander Kelle
2009-09-10T08:44:13Z
置信度 0.70
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crossref
2016-04-07T23:28:32Z
置信度 0.70
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crossref
Gi Na Lee, Jonguk Na
2013-05-17T04:03:06Z
置信度 0.70
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crossref
2016-12-06T12:18:04Z
置信度 0.70
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crossref
2018-05-02T09:18:30Z
置信度 0.70
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crossref
Paul Rabinow, Gaymon Bennett
2009-10-08T22:50:37Z
置信度 0.70
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crossref
Devangi Zala, Shuvomoy Banerjee, Ansh Mehta, Anand K. Tiwari
2026-05-17T22:08:37Z
置信度 0.70