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• Potential Applications of Synthetic Biology in Marine Microbial Functional Ecology and Biotechnology
  crossref Guangyi Wang, Juanita Mathews 2009-09-22T20:46:30Z 置信度 0.70
• Chapter 6. Modular design strategies for protein sensors and switches
  crossref Maarten Merkx 2014-06-02T14:28:59Z 置信度 0.70
• Microfluidics for Synthetic Biology
  crossref M.S. Ferry, I.A. Razinkov, J. Hasty 2012-05-17T10:16:25Z 置信度 0.70
• The Ethics of Synthetic Biology: Outlining the Agenda
  crossref Anna Deplazes, Agomoni Ganguli-Mitra, Nikola Biller-Andorno 2009-09-10T12:44:13Z 置信度 0.70
• DASH: A versatile and high-capacity gene stacking system for plant synthetic biology
  Abstract DNA assembly systems based on the Golden Gate method are popular in synthetic biology but have several limitations: small insert size, incompatibility with other cloning platforms, DNA domestication requirement, generation of fusion scars, and lack of…
  preprints Chengsong Zhao, Anna N. Stepanova, Jose M. Alonso 2025 置信度 0.74
• Synthetic Biology at Work
  crossref Sergio Carrà 2018-10-10T19:34:47Z 置信度 0.70
• Synthetic Biology for Treatment of Cancer
  crossref Huzaifa Ibrahim, Shah Fawad, Hafsa Adnan, Jayakumar Rajadas 2026-05-18T00:43:34Z 置信度 0.70
• Systems Biology and Microgravity Effects on Living Organisms
  crossref Bizzarri M 2015-01-05T11:27:45Z 置信度 0.70
• Synthetic Biology Standards and Methods of DNA Assembly
  crossref Philip Weyman, Yo Suzuki 2016-04-07T23:28:32Z 置信度 0.70
• Evolutionary Synthetic Biology
  crossref Sergio G. Peisajovich 2012-03-29T19:13:55Z 置信度 0.70
• Chapter 9. Functional frontiers: engineering biomimetic interfaces
  crossref Hana Robson Marsden, Alexander Kros 2014-06-02T14:28:59Z 置信度 0.70
• Designing Synthetic Biology
  crossref Christina M. Agapakis 2013-10-24T14:23:01Z 置信度 0.70
• Biomimetic Approaches in Synthetic Biology
  crossref Renuka Suravajhala, Aakanksha Kumar, Harshavardhan Reddy Burri, Babita Malik 2020-04-13T11:02:57Z 置信度 0.70
• Theory and Construction of Semi-Synthetic Minimal Cells
  crossref 2016-04-07T23:28:32Z 置信度 0.70
• Synthetic Biology for Treatment of Metabolic Disorders
  crossref Santhosh Kumar Yasam, Joice Kuzhipalayil Joseph, Senthilkumar Rajagopal 2026-05-17T23:45:48Z 置信度 0.70
• Hindrances to the Efficient and Stable Expression of Transgenes in Plant Synthetic Biology Approaches
  crossref Ana Pérez-González, Elena Caro 2016-09-08T14:55:01Z 置信度 0.70
• Synthetic Biology for Deciphering Cancer Mechanisms
  crossref Gayatri Shamsunder Salgaonkar, Dishita Dutta, Vinayak Shishodia, Dhruv Kumar 2026-05-18T00:39:05Z 置信度 0.70
• Computational Protein Design Methods for Synthetic Biology
  crossref Pablo Carbonell, Jean-Yves Trosset 2014-12-08T09:39:11Z 置信度 0.70
• An introduction of synthetic biology and important milestones
  crossref Subhajit Das, Ayan Chatterjee 2025-11-08T11:00:56Z 置信度 0.70
• Synthetic Biology for Treatment of Blood Disorders
  crossref Ingita Dey Munshi, Ruchika Riya Tirkey, Indra Mani 2026-05-18T00:36:59Z 置信度 0.70
• Futures: Imagining tomorrow shapes today
  This chapter explores the interplay between present decisions and future outcomes in synthetic biology, highlighting the dynamic relationship between scientific promises and their real-world implications. Using a case study on gene drives, it examines how subt…
  crossref Emma Frow, Robert Smith, Lalitha Sundaram 2026-08-04T16:44:41Z 置信度 0.70
• Ensuring a Safe Future in the Age of Synthetic Biology
  crossref Leyma Pérez, De Haro 2024-08-08T18:41:23Z 置信度 0.70
• Design and Characterization of Genetic Parts in Synthetic Biology
  crossref Ali Samy Abdelaal, Fernando G. Sorroche 2026-05-18T00:48:35Z 置信度 0.70
• Recognizing the Patients Phenotype through Systems Biology
  crossref Bizzarri M Palombo A 2015-05-06T08:55:25Z 置信度 0.70
• Applications of synthetic biology in biomedicine.
  Based on the principles of engineering reconstruction and programmable design, synthetic biology is driving a paradigm shift in biomedical diagnosis and therapy from conventional models toward intelligent and precision medicine. By constructing artificial gene…
  pubmed Li CX, Liu ZX, Lin YF, Wang ZH 等 2026 置信度 0.82
• Site-specific recombinases and their essential role in synthetic biology.
  Site-specific recombinases, enzymes that bind to specific DNA attachment sites and catalyze DNA rearrangement reactions resulting in permanent and heritable genetic modifications, have emerged as powerful tools in synthetic biology. Their broad applications in…
  pubmed Ba F, Sun Q 2026 置信度 0.82
• Application and progress of synthetic biology technology in amino acid production.
  europepmc 2026 置信度 0.80
• [Construction and practice of a talent training model for synthetic biology].
  europepmc 2026 置信度 0.80
• DIPTAR: A synthetic biology platform for functional interrogation of protein degradation
  Abstract Protein degradation regulates cellular homeostasis, yet many degradation events are difficult to study because they lack a readily selectable phenotype. Here, we develop Degradation-Induced Pyroptosis TArgeting Receptors (DIPTAR), a modular synthetic …
  europepmc Patrick M. Exconde, William Yoo, Madhura Kulkarni, Ashutosh B. Mahale 等 2026 置信度 0.80
• Navigating regulatory fragmentation in the convergence of synthetic biology, artificial intelligence, and automation.
  europepmc 2026 置信度 0.80
• Engineered platforms for melanogenesis research: Bridging synthetic biology, bioengineering, and biomimetics.
  Melanogenesis, the biological process responsible for melanin synthesis, plays a critical role in determining skin pigmentation and providing photoprotection. Dysregulation of this pathway leads to a wide range of pigmentary disorders that affect a significant…
  pubmed Anika B, Tarkunde A, Swarna BS, Nayak UY 等 2026 置信度 0.82
• Integrative framework for selecting gene delivery technologies in microalgae for synthetic biology.
  Microalgae are attractive hosts for synthetic biology due to their ability to produce high-value compounds. Although gene delivery methods have been established in microalgae, their adaptation to synthetic biology applications remains limited. This study provi…
  pubmed Cho M, Sung YJ 2026 置信度 0.82
• Synthetic biology strategies for engineering microbial therapeutics in metabolic diseases and cancer.
  Synthetic biology has redefined the therapeutic role of microbes, transforming them from passive commensals or delivery vehicles into programmable living therapeutics capable of sensing, computing, and actuating within host tissues. This transformation is part…
  pubmed Onoja BA, Agada SA, Aggad WS, Almohaimeed HM 等 2026 置信度 0.82
• Thailand's Synthetic Biology Strategy.
  ABSTRACT Synthetic biology in Thailand offers vast opportunities, from enhancing agriculture and food production to developing sustainable chemicals and biomaterials, advancing renewable energy and advancing pharmaceutical and healthcare services. Although Tha…
  europepmc Sirinya Lim, Watcharin Meerod, Sasiphen Hathaichoti, Kantapitch Preedakorn 2026 置信度 0.80
• Biological and synthetic biology strategies for polystyrene degradation: mechanisms, microbial advances, and circular economy perspectives.
  Polystyrene (PS), a petroleum-based synthetic polymer which is extensively used in packaging, insulation, and consumer goods, has emerged as a major environmental pollutant. This report provides a comprehensive overview of recent advances in biological and syn…
  pubmed Nazir M, Rehman A, Ibrahim AH, Al-Rawi SS 等 2026 置信度 0.82
• Automated, modular assembly of reconstituted cell-free systems from in vitro-produced components.
  High-performance cell-free protein synthesis has transformative potential for synthetic biology, yet the prohibitive costs of Protein synthesis Using Recombinant Elements kits and the labor intensity of in-house preparation have restricted accessibility and sc…
  pubmed Chae B, Park H, Jeon H, Lee J 等 2026 Jun 5 置信度 0.82
• Hollow-fibre biomanufacturing and cell-free engineering of HEK293 extracellular vesicles.
  Extracellular vesicles (EVs) are lipid-delimited particles produced by most cell types that can have therapeutic effects. Cell-free gene expression systems can be used to produce membrane proteins in vitro, which can integrate with exogenously added EVs. To ad…
  pubmed Kelwick RJR, Webb AJ, Heliot A, Freemont PS 2026 Jun 4 置信度 0.82
• Assembly and Reactions of Artificial Metalloenzymes in Streptomyces albus.
  Artificial metalloenzymes (ArMs) expand the suite of synthetically valuable, new-to-nature biocatalytic reactions. Integrating these enzymes into biosynthetic pathways enables reactions not found in nature to occur in living cells with the intermediates or pro…
  pubmed Chakraborty S, Hwang S, Huang J, Chen D 等 2026 Jun 17 置信度 0.82
• Expanding the genetic toolset: using serine recombinases to integrate riboregulatory elements into industrially relevant microbial chassis.
  To realize the full potential of biomanufacturing, the breadth of industrial microbes used to consume diverse feedstock and generate bioproducts needs to expand. As such, portable tools are required that can be used by multiple hosts for straightforward genomi…
  pubmed Wozniak KL, Carroll AL, Davison SA, Jha RK 等 2026 Jan 8 置信度 0.82
• Describing and Designing Microbial Community Metabolic Models In Silico: A Comprehensive Protocol Utilizing FLYCOP.
  Microbial communities play crucial roles in multiple natural and engineered environments, contributing to biogeochemical cycling, waste treatment, and biotechnological processes. Much like for single organisms, genome-scale metabolic models (GEMs) have become …
  pubmed Del Ramo A, Granado DSL, Nogales J 2026 置信度 0.82
• PromoterAtlas: decoding regulatory sequences across Gammaproteobacteria using a transformer model.
  Recent advances in deep learning, particularly transformer architectures, have improved computational approaches for biological sequence analysis. Despite these advances, computational models for bacterial promoter prediction have remained limited by small dat…
  pubmed Coppens L, Ledesma-Amaro R 2026 May 15 置信度 0.82
• Decoding bacterial transcriptional regulatory networks through integrated multi-omics datasets for artificial intelligence-driven design of programmable biological systems.
  Advances in next-generation sequencing (NGS) have provided refined insight into bacterial transcriptional regulatory networks (TRNs) on a genome-wide scale. The integration of genomic and transcriptomic datasets has clarified the architecture of TRNs, revealin…
  pubmed Bang I, Kim J, Jang M, Park JY 等 2026 Jun 置信度 0.82
• Alternative antibiotic regimens improve palatability and welfare in mice for gut bacterial depletion.
  Here we evaluated vancomycin-neomycin or enrofloxacin-ampicillin treatments as alternatives to the poorly tolerated ampicillin-metronidazole-vancomycin-neomycin cocktail for gut bacterial depletion in mice. Both regimens were well tolerated in drinking water a…
  pubmed Abaakil K, Bi Y, Firmenich L, Yang X 等 2026 Jul 置信度 0.82
• TridentSynth: a webtool for the retrosynthesis of molecules using chimeric type I polyketide synthases and chemoenzymatic pathways.
  The design of pathways to synthesize valuable molecules remains a central challenge in chemistry and biotechnology. Several computational retrosynthesis tools have been developed to address this problem, but their scope is often confined only to reactions in e…
  pubmed Chainani Y, Guilarte-Silva M, Roberts K, Pate S 等 2026 Jul 11 置信度 0.82
• Engineering photosynthetic microorganisms in biofoundries: challenges and opportunities.
  Photosynthetic microorganisms can convert sunlight and CO₂ directly into biomass and bioproducts. Yet, most biofoundries still optimize heterotrophic chassis reliant on agricultural sugars, limiting impact on global decarbonization. This review argues t…
  pubmed Douchi D, Husser M, Jomat L, Marchand CH 等 2026 Jun 置信度 0.82
• Discovery and engineering of a new BvCas12a nuclease for mammalian genome editing and nucleic acid detection.
  pubmed Shen X, Huang L, Wang D, Qi C 等 2026 Apr 16 置信度 0.82
• Metric-driven biosensors for analyte detection.
  Protein-based biosensors are vital for environmental monitoring, diagnostics, and bioprocess control, yet many stall in the transition from laboratory to field because they fail to meet performance metrics. This review focuses on the adoption of a results-orie…
  pubmed Granja-Travez RS, Shin SM, Miller JE, Jha RK 等 2026 Jun 置信度 0.82
• Invited review: Turning dairy food waste into valuable products via yeast bioproduction.
  Dairy food waste (DFW) is a major global issue, with estimates indicating that almost 20% of production is wasted in some regions. Such losses result in economic and environmental challenges but also create opportunities to recover valuable nutrients, reduce c…
  pubmed Gargiulo JI, Llorente B, Fabian B, Wlodarczyk A 等 2026 Jul 置信度 0.82
• Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger.
  Despite revolutionizing fungal genetic engineering, conventional CRISPR/Cas9-mediated knockouts rely on DNA double-strand breaks (DSBs), which can cause unwanted insertions and deletions, chromosomal abnormalities, and cytotoxicity. Base editors such as adenin…
  pubmed Yuan G, Deng S, Dai Z, Hofstad BA 等 2026 Mar 26 置信度 0.82
• Biosensor-guided directed evolution of aldehyde dehydrogenase for improved adipic acid production from caprolactone.
  Biological production of adipic acid, a crucial building block in nylon and polymer manufacturing, from renewable feedstocks has attracted attention as a sustainable alternative to the traditional petrochemical production. The enzymatic conversion of ε-c…
  pubmed Kim H, Moon SY, Park JY, Baek SH 等 2026 Mar 23 置信度 0.82
• Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.
  Agrobacterium is not only a costly plant pathogen but is also an essential tool for plant transformation. Though Agrobacterium -mediated transformation (AMT) has been heavily studied, its polygenic nature and complex transcriptional regulation make identificat…
  pubmed Thompson MG, Kirkpatrick LD, Szarzanowicz MJ, Geiselman GM 等 2026 Apr 17 置信度 0.82
• Host analysis-guided selection and targeted engineering (HASTE) of Lipomyces tetrasporus for the conversion of CO(2)-derived feedstocks.
  Cost-competitive bioproduction calls for utilizing cellulosic sugars or acetate from electro-reduction of CO 2 . Here, we identified a novel Lipomyces tetrasporus strain (i.e., NRRL Y-11562 from 24 Lipomyces species) capable of effectively assimilating diverse…
  pubmed Xiao Z, Czajka JJ, Alvarez J, Yu Y 等 2026 Jun 置信度 0.82
• CasY7: An optimized Cas12i system for enhanced genome editing in monocot crops.
  The CRISPR-Cas12 family nucleases, particularly the Cas12i subtypes, are considered promising alternatives to Cas9 for genome editing in plants. We previously developed a new Cas12i variant, CasY7, which has been successfully applied in clinical trials; its pe…
  pubmed Zhong D, Dong Y, Pan H, Fu Y 等 2026 Mar 11 置信度 0.82
• Prediction of non-intuitive metabolic targets with bayesian metabolic control analysis to improve 3-hydroxypropionic acid production in Aspergillus niger.
  Development of efficient bioconversion processes is limited by the ability to predictably improve metabolic flux. Here we deployed Bayesian Metabolic Control Analysis as a platform to integrate multi-omics data with metabolic modeling and evaluated its ability…
  pubmed Dai Z, Zucker JD, Han Y, Mahserejian S 等 2026 置信度 0.82
• Optimizing linker length of base editors for precise crop breeding and gene therapy.
  Base editing enables efficient nucleotide conversions without inducing DNA double-strand breaks (DSBs) or requiring exogenous donor DNA templates. However, its broader editing window often causes bystander editing, increasing the risk of unintended mutations. …
  pubmed Shi Y, Yuan Y, Qin L, Zhou F 等 2026 Jun 置信度 0.82
• mRNA Vaccine Against Japanese Encephalitis Virus Genotype IV Protects Against Lethal Infection.
  In 2022, Australia saw an unprecedented outbreak of Japanese encephalitis virus genotype IV (JEV GIV). The outbreak involved 42 human cases with 7 fatalities, as well as affecting >80 pig farms in New South Wales and Queensland. Herein, we designed, constru…
  pubmed Cox AL, Nguyen W, Wales-Earl L, Tang B 等 2026 Jan 28 置信度 0.82
• Editorial: Artificial Intelligence in Ion Mobility Spectrometry-Mass Spectrometry for Omics Research.
  pubmed Bilbao A, Zheng X 2026 Mar 置信度 0.82
• Correction to "Thermophilic Chassis-Enabled High-Throughput Selection of a Thermostable Fluorogenic Reporter".
  pubmed Shin SM, Triola EH, Granja-Travez RS, López CA 等 2026 Mar 20 置信度 0.82
• ML-ABC: Machine-learning assisted Approximate Bayesian Computation for efficient calibration of agent-based models for pandemic outbreak analysis.
  Mathematical modelling with agent-based models (ABMs) has gained popularity during the COVID-19 pandemic, but their complexity makes efficient and robust calibration to data challenging, particularly when applying Bayesian methods to quantify parameter uncerta…
  pubmed Bayley T, Ward T, Sturman F, Das A 等 2026 Mar 置信度 0.82
• A surrogate-selection-enhanced TnpBmax platform enables efficient genome editing in rice and tomato.
  TnpB, a compact RNA-guided nuclease ancestral to Cas12, is optimized for plant genome editing. A 99-nt enhanced RNA and T5 exonuclease fusion generate TnpBe5, boosting rice editing 2.5-fold. Coupling with a single-strands annealing (SSA)-responsive HYYG surrog…
  pubmed Li E, Xie J, Li X, Zheng Z 等 2026 Jan 28 置信度 0.82
• High-throughput yeast engineering in biofoundries: towards autonomous and scalable synthetic biology.
  High-throughput yeast engineering is being transformed by biofoundries that integrate automation, artificial intelligence (AI), and standardized workflows. This review examines how these facilities accelerate strain development through the Design-Build-Test-Le…
  pubmed Martinez JPO, Speight RE 2026 Jan 5 置信度 0.82
• Connector-enabled integration of Golden Gate Assembly and yeast recombination for streamlined multigene pathway construction in the biofoundry workflow.
  Rewiring the metabolic flux for efficient microbial conversion requires robust, scalable gene assembly. However, conventional gene assembly approaches are labor-intensive, highly experience-dependent, and require extensive expertise to ensure reproducibility a…
  pubmed Seong MJ, Kim H, Lee H, Kim H 等 2026 May 置信度 0.82
• Guest Editorial: Ion Mobility-Mass Spectrometry in Omics.
  pubmed Bilbao A, Causon T 2026 Apr 置信度 0.82
• Systematic optimization of TF-based carboxylic acid biosensors in cell-free system.
  Rapid and precise detection of small-molecule metabolites is crucial for optimizing the bioproduction processes. Cell-free systems (CFSs) offer an ideal platform for developing such biosensors due to their speed and suitability for automation. However, transcr…
  pubmed Kim W, Kim S, Jeung K, Lim HG 等 2026 Apr 1 置信度 0.82
• Decoding the full chemical catalytic cycle of class I PHA synthase: A QM/MM metadynamics perspective.
  The accumulation of plastic waste continues to pose a significant threat to ecosystems, driven by inefficient recycling processes and inadequate waste management. Polyhydroxyalkanoates (PHAs) have emerged as promising biodegradable alternatives, owing to their…
  pubmed Kim Y, Lee H, Lee H, Han JW 2026 Jan 置信度 0.82
• From Natural Discovery to AI-Guided Design: A Curated Collection of Compact Enhancers for Crop Engineering.
  Precise transgene-free gene upregulation remains a challenge in crop biotechnology, as conventional enhancers often exceed CRISPR-mediated knock-in size constraints and face regulatory hurdles. Here we establish a foundational cross-species resource of compact…
  pubmed Yao Q, Gao J, Wang K, Liu Y 等 2026 Mar 置信度 0.82
• Single cell profiling framework reveals metabolic subpopulations as drivers of bioproduction heterogeneity.
  Heterogeneity within clonal cell populations remains a critical bottleneck within bioprocess engineering, notably by undermining bioproduction yields. Efforts to mitigate its impact have, however, been hampered by technological difficulties quantifying metabol…
  pubmed Savigny J, Shabestary K, Portela M, Klemm C 等 2025 Dec 21 置信度 0.82
• Challenges and opportunities for establishing biofoundries in Latin America.
  Biofoundries are transforming biotechnology by automating design-build-test-learn (DBTL) cycles that accelerate biological design and innovation. While globally recognized as strategic infrastructures for the bioeconomy, they remain underrepresented in Latin A…
  pubmed Giuseppe PO, Sampaio NM, Junqueira TL, Mandelli F 等 2026 Feb 置信度 0.82
• A synthetic cell phage cycle.
  Viral infection of living cells, exemplified by bacteriophage interaction with bacteria, is fundamental to biology and universal across living systems. Here, we establish an all-cell-free viral cycle where T7 phages infect synthetic cells, equipped with lipopo…
  pubmed Levrier A, Soudier P, Garenne D, Izri Z 等 2025 Dec 15 置信度 0.82
• Design biology and mind-body health.
  Recent advances in design biology, including artificial cells, DNA nanostructures, artificial intelligence (AI)-driven molecular design, biofoundries, and next-generation genome editing, are transforming the health sciences of mind and body (HS-MB). Moving bey…
  pubmed Kumagai K, Chiba A, Yajima H 2025 Dec 置信度 0.82
• Editor's Choice JAAG: a JSON input file assembler for AlphaFold 3 with glycan integration.
  Standalone AlphaFold 3 (AF3) models proteins, post-translational modifications, and ligands (including glycans) from a single JSON input file. Plausible glycan stereochemistry is more consistently achieved when monosaccharides are specified as Chemical Compone…
  pubmed Huang C, Moremen KW 2025 Dec 17 置信度 0.82
• Automated Genetic Manipulation for the Construction of Pichia pastoris Cell Factories.
  As a nonmodel microorganism, Pichia pastoris has unique advantages in recombinant protein expression and natural product biosynthesis. The development of synthetic biology has provided key technical support for the construction of microbial cell factories. How…
  pubmed Zuo Y, Jin L, Zhang J, Gao J 等 2025 Nov 27 置信度 0.82
• Techno-economic assessment-guided biofoundry for microbial strain development.
  A biofoundry integrates laboratory automation with Design-Build-Test-Learn (DBTL) workflows to accelerate strain development for sustainable manufacturing. Quantifying the economic efficiency of automated processes remains challenging. Here, we define the robo…
  pubmed Heo YB, Ko SC, Keasling JD, Woo HM 2026 Apr 置信度 0.82
• Perspectives for artificial intelligence in bioprocess automation.
  Recent advances in artificial intelligence (AI) have rapidly changed the lab automation landscape, promoting self-driving laboratories (SDLs) that enable autonomous scientific discovery. These trends are increasingly applied in bioprocess development, yet…
  pubmed Helleckes LM, Putz S, Gupta K, Franzreb M 等 2026 Feb 置信度 0.82
• Surface wetting is a key determinant of α-synuclein condensate maturation.
  α-Synuclein condensates can mature into amyloid fibrils, demonstrating a link between phase separation and amyloid aggregation. However, the mechanisms driving this maturation are not fully understood, particularly in the context of pathological post-tra…
  pubmed Thrush RJ, Vadukul DM, Allerton SC, Storch M 等 2025 Nov 27 置信度 0.82
• Governing Synthetic Biology: A Co-Evolutionary Framework for Sustainable Innovation.
  Synthetic biology has rapidly evolved from laboratory-based research to a core technology driving biomanufacturing, industrial innovation, and the global bioeconomy. Technological advancements such as next-generation sequencing, DNA synthesis, novel genome edi…
  pubmed Kim H, Lee J, Cho Y, Kim H 等 2025 Nov 26 置信度 0.82
• Efflux pump knockout minimizes crosstalk and boosts response in transcription factor-based biosensors.
  Cellular transport systems are key determinants of intracellular molecule concentrations and can be utilized to modulate the responsiveness of transcription factor (TF)-based biosensors. Efflux pumps facilitate the export of small molecules; therefore, their a…
  pubmed Lee J, Yoo BH, Jeon E, Kong H 等 2025 Nov 12 置信度 0.82
• Advancing arabinose-based bioproduction in Yarrowia lipolytica by integrating metabolic engineering and adaptive laboratory evolution.
  The oleaginous yeast, Yarrowia lipolytica has gained interest as a biotechnological chassis to produce foods, chemicals, pharmaceuticals, and biofuels. To reduce production costs and sustainability, inexpensive and abundant feedstocks such as lignocellulose mu…
  pubmed Rafieenia R, Fu J, Hapeta P, Storch M 等 2026 Mar 置信度 0.82
• Recent advances in AI-enabled automation of DNA assembly in biofoundries.
  Biofoundries are transforming synthetic biology, with the DNA assembly workflow being critical for successful biofoundry operation. This review examines recent advances in automated DNA assembly strategies for biofoundry, focusing on three key perspectives. Fi…
  pubmed Kim JH, Kim JH, Kim SD, Kim H 等 2025 Dec 置信度 0.82
• Open Enzyme Database: a community-wide repository for sharing enzyme data.
  Enzymes are the molecular machines of life and play an indispensable role in numerous biotechnological and biomedical applications. Despite the availability of a few enzyme databases, their utility is often hindered by limitations such as infrequent updates, l…
  pubmed Yuan L, Bianchi DM, Arneson K, Guo B 等 2026 Jan 6 置信度 0.82
• Architectures of emerging biofoundry platforms for synthetic biology.
  Biofoundries are integrated, automated platforms that accelerate synthetic biology applications by facilitating high-throughput and labor-intensive experiments. They incorporate robotic systems, analytical instruments, and software for workflow design, executi…
  pubmed Heo YB, Park JS, Woo HM 2025 Dec 置信度 0.82
• Building a lignin biofoundry: a review.
  Lignin, a complex biopolymer, holds significant promise for sustainable bioconversion into high-value products and chemicals; however, its recalcitrance, variable composition, and low degradation rates limit industrial application. Naturally sourced enzymes re…
  pubmed Bosman CE, Tyhoda L, Görgens JF, Trollope KM 2025 Dec 置信度 0.82
• A green biomass pretreatment strategy to produce 3-hydroxypropionic acid and ethanol with engineered Saccharomyces cerevisiae, Rhodosporidium toruloides, and Issatchenkia orientalis.
  Chemical and water overconsumption involved in solid-liquid separation and post-washing after biomass pretreatment pose significant challenges to scaling up the production of platform chemicals and biofuels. This study explored a novel one-pot biomass valoriza…
  pubmed Zhang M, Hwang HJ, Jeong D, Jia L 等 2026 Feb 置信度 0.82
• A Lactose-Based Kluyveromyces lactis Cell-Free Protein Synthesis System.
  Yeasts have long been celebrated for their metabolic prowess, in particular, their ability to transform grain and fruit into valuable food products. In recent decades, humans have exploited them for pharmaceutical applications driven by advances in metabolic e…
  pubmed Shivakumar T, Das A, Bussoletti Panizo MV, Storch M 等 2025 Oct 23 置信度 0.82
• Aromatic compound production by Corynebacterium glutamicum: unified strategies at the core and diversity by extension.
  Aromatic compounds are an important class of natural substances with a wide range of physiological functions and, thus, applications in the food, feed, fine chemical, and plastics industries or as pharmaceuticals. As a prime player in industrial amino acid pro…
  pubmed Junker N, Poethe SS, Wendisch VF 2025 Dec 置信度 0.82
• High-Throughput Engineering and Modification of Non-Ribosomal Peptide Synthetases Based on Golden Gate Assembly.
  Non-ribosomal peptide synthetases (NRPS) are multimodular enzymes that produce complex peptides with diverse biological activities, potentially being used as clinical drugs. However, the pharmaceutical applications of such natural peptides often require furthe…
  pubmed Podolski A, Lindeboom TA, Präve L, Dehghan M 等 2025 Dec 1 置信度 0.82
• Engineering a Robust Escherichia coli W Platform for Scalable Production of Flavonoid-O-Glucosides.
  Flavonoids are valuable for pharmaceutical, cosmetic and food applications. However, poor solubility and bioavailability limit their widespread use. Biotechnological glycosylation of flavonoids helps address these limitations, but such bioprocesses remain cons…
  pubmed Carranza-Saavedra D, Torres-Bacete J, Bouju E, Nuccio S 等 2025 Oct 置信度 0.82
• Thermophilic Chassis-Enabled High-Throughput Selection of a Thermostable Fluorogenic Reporter.
  Thermostable proteins show increased shelf life and performance at elevated temperatures and under harsh conditions, resulting in lower costs for various industrial and biotechnological applications. However, due to a limited understanding of the relationship …
  pubmed Shin SM, Triola EH, Granja-Travez RS, López CA 等 2025 Oct 17 置信度 0.82
• Production of triacetic acid lactone from oleic acid by engineering the yeast Candida viswanathii.
  Triacetic acid lactone (TAL) is a promising platform chemical to produce valuable compounds. The development of engineered microbial hosts to efficiently produce TAL from lipid-containing waste streams could be a cost-effective, sustainable and environmentally…
  pubmed Ghosh S, Hutagalung A, Gao Y, Flores JE 等 2025 Oct 6 置信度 0.82
• mzPeak: Designing a Scalable, Interoperable, and Future-Ready Mass Spectrometry Data Format.
  Advances in mass spectrometry (MS) instrumentation, including higher resolution, faster scan speeds, and improved sensitivity, have dramatically increased the data volume and complexity. The adoption of imaging and ion mobility further amplifies these challeng…
  pubmed Van Den Bossche T, Alexandrov T, Bilbao A, Bittremieux W 等 2025 Nov 7 置信度 0.82
• Machine Learning-Guided Identification of PET Hydrolases from Natural Diversity.
  The enzymatic depolymerization of poly-(ethylene terephthalate) (PET) is emerging as a leading chemical recycling technology for waste polyester. As part of this endeavor, new candidate enzymes identified from natural diversity can serve as useful starting poi…
  pubmed Norton-Baker B, Komp E, Gado JE, Denton MCR 等 2025 Sep 19 置信度 0.82
• Accessible, uniform protein property prediction with a scikit-learn based toolset AIDE.
  Protein property prediction via machine learning with and without labeled data is becoming increasingly powerful, yet methods are disparate and capabilities vary widely over applications. The software presented here, "Artificial Intelligence Driven protein Est…
  pubmed Komp E, Johansson KE, Gauthier NP, Gado JE 等 2025 Oct 2 置信度 0.82
• Staying productive under pressure: systems evaluations of β-carotene production in Yarrowia lipolytica under continuous fermentation.
  Scaling biomanufacturing from laboratory to industrial scale poses significant challenges, especially for continuous fermentation. This study investigates these challenges using a β-carotene-producing Yarrowia lipolytica strain. Through fermentation expe…
  pubmed Worland AM, Xu VA, Duran MF, Gitman P 等 2026 Jan 置信度 0.82
• Inducible flippase-mediated metabolic engineering of Rhodosporidium toruloides for enhanced 3-hydroxypropionic acid production from corn stover hydrolysate.
  Rhodosporidium toruloides has gained increasing interests as a promising non-model host organism to produce a wide range of bioproducts from lignocellulosic biomass. Increasing the bioproduct titers, rates, and yields remains a challenge, largely due to a lack…
  pubmed Hwang HJ, Adamczyk PA, Gladden JM, Liu D 2026 Jan 置信度 0.82
• Proteomic insights into the physiology and metabolism of oleaginous yeasts and filamentous fungi.
  Fungi are vital to the bioeconomy, serving as key producers of food, beverages, biofuels, and medicines, while also acting as essential resource recyclers in ecosystem management. For nearly a century, oleaginous yeast and filamentous fungi have been explored …
  pubmed Gluth A, Trejo JB, Czaijka JJ, Deng S 等 2025 置信度 0.82
• Automated and Programmable Cell-Free Systems for Scalable Synthetic Biology with a Focus on Biofoundry Integration.
  Cell-free protein synthesis (CFPS) has been used as a transformative technology in synthetic biology, providing a programmable, scalable, and automation-compatible platform for biological engineering. Freed from the limitations of cell viability and growth, CF…
  pubmed Jun JS, Hong S, Park JH, Shin J 等 2025 Sep 16 置信度 0.82
• Molecular and structural basis for nitrosoglutathione-dependent redox regulation of triosephosphate isomerase from Chlamydomonas reinhardtii.
  Protein S-nitrosylation is a reversible redox-based post-translational modification that plays an important role in cell signaling by modulating protein function and stability. At the molecular level, S-nitrosylation consists of the formation of a nitrosothiol…
  pubmed Meloni M, Mattioli EJ, Fanti S, Peppi GME 等 2026 Jan 置信度 0.82
• Semi-automated biofoundry workflows for sequence coevolution-guided isoprene synthase engineering.
  Biofoundries serve as transformative platforms for accelerating the engineering of enzymes and microorganisms toward biomanufacturing. In this study, we developed scalable enzyme engineering workflows tailored for biofoundry applications, focusing on isoprene …
  pubmed Emelianov G, Song DU, Kamath A, Kim H 等 2026 Jan 置信度 0.82
• Species-specific detection of Schistosoma japonicum using the 'SNAILS' DNA-based biosensor.
  The neglected tropical disease schistosomiasis continues to be a global health concern, especially in low- and middle-income countries, with at least 250 million people infected worldwide and a further 779 million at risk of infection. Schistosoma japonicum, w…
  pubmed Webb AJ, Zhao QP, Allan F, Kelwick RJR 等 2025 Aug 30 置信度 0.82
• Modeling glycans with AlphaFold 3: capabilities, caveats, and limitations.
  Glycans are complex carbohydrates that exhibit extraordinary structural complexity and stereochemical diversity while playing essential roles in many biological processes, including immune regulation, pathogen recognition, and cell communication. In humans, mo…
  pubmed Huang C, Kannan N, Moremen KW 2025 Sep 3 置信度 0.82