合成生物与生物制造 · 全部实体（第 89 / 100 页）

本页收录第 8801–8900 条，共 10000 条 · 返回 合成生物与生物制造 首页

• Enzyme engineering and its industrial applications
  Recently, there has been an increase in the demand for enzymes with modified activity, specificity, and stability. Enzyme engineering is an important tool to meet the demand for enzymes adjusted to different industrial processes. Knowledge of the structure and…
  openalex Isabela Victorino da Silva Amatto, Nathália Gonsales da Rosa-Garzon, Flávio Antônio de Oliveira Simões, Fernanda L.B. Santiago 等 2021-02-08 置信度 0.72 Biochemical engineeringEnzymeProtein engineeringRational designBiotechnology
• Functionalized Graphene Oxide in Enzyme Engineering: A Selective Modulator for Enzyme Activity and Thermostability
  The understanding of interactions between nanomaterials and biomolecules is of fundamental importance to the area of nanobiotechnology. Graphene and its derivative, graphene oxide (GO), are two-dimensional (2-D) nanomaterials with interesting physical and chem…
  openalex Liling Jin, Kai Yang, Kai Yao, Shuai Zhang 等 2012-05-10 置信度 0.72 ThermostabilityProtein engineeringNanomaterialsMaterials sciencePolyethylene glycol
• RECENT ADVANCES IN RATIONAL APPROACHES FOR ENZYME ENGINEERING
  Enzymes are an attractive alternative in the asymmetric syntheses of chiral building blocks. To meet the requirements of industrial biotechnology and to introduce new functionalities, the enzymes need to be optimized by protein engineering. This article specif…
  openalex Kerstin Steiner, Helmut Schwab 2012-09-01 置信度 0.72 Protein engineeringRational designEnzymeBiochemical engineeringSubstrate specificity
• Engineering new catalytic activities in enzymes
  openalex Kai Chen, Frances H. Arnold 2020-01-20 置信度 0.72 EnzymeCatalysisChemistryBiochemistryBiochemical engineering
• Enzyme engineering: rational redesign versus directed evolution
  openalex Ridong Chen 2001-01-01 置信度 0.72 Directed evolutionScope (computer science)Rational designBiochemical engineeringProtein engineering
• High-throughput screening technologies for enzyme engineering
  openalex Chelsea K. Longwell, Louai Labanieh, Jennifer R. Cochran 2017-06-16 置信度 0.72 Compartmentalization (fire protection)High-throughput screeningEnzymeThroughputDirected evolution
• Enzyme Engineering for In Situ Immobilization
  Enzymes are used as biocatalysts in a vast range of industrial applications. Immobilization of enzymes to solid supports or their self-assembly into insoluble particles enhances their applicability by strongly improving properties such as stability in changing…
  openalex Fabian B. H. Rehm, Shuxiong Chen, Bernd H. A. Rehm 2016-10-14 置信度 0.72 BiocatalysisImmobilized enzymeEnzymePolyhydroxyalkanoatesProtein engineering
• Role of distal sites in enzyme engineering
  openalex Jie Gu, Yan Xu, Yao Nie 2023-01-05 置信度 0.72 Protein engineeringActive siteFunction (biology)Rational designMechanism (biology)
• TECHNOLOGY PROSPECTING ON ENZYMES: APPLICATION, MARKETING AND ENGINEERING
  Enzymes are protein molecules functioning as specialized catalysts for chemical reactions. They have contributed greatly to the traditional and modern chemical industry by improving existing processes. In this article, we first give a survey of representative …
  openalex Shuang Li, Xiaofeng Yang, Shuai Yang, Muzi Zhu 等 2012-09-01 置信度 0.72 Biochemical engineeringEnzymeBiotechnologyBusinessComputer science
• Engineering herbicide resistance in plants by expression of a detoxifying enzyme
  openalex Marc De Block, Johan Botterman, Martine Vandewiele, Jan Dockx 等 1987-09-01 置信度 0.72 BiologyEnzymeResistance (ecology)Expression (computer science)Herbicide resistance
• From directed evolution to computational enzyme engineering—A review
  Abstract Nature relies on a wide range of enzymes with specific biocatalytic roles to carry out much of the chemistry needed to sustain life. Enzymes catalyze the interconversion of a vast array of molecules with high specificity—from molecular nitrogen fixati…
  openalex Ratul Chowdhury, Costas D. Maranas 2019-10-23 置信度 0.72 Biochemical engineeringChemistryEnzymeDirected evolutionBiocatalysis
• Enzyme engineering: reaching the maximal catalytic efficiency peak
  ) by several orders of magnitude. However, optimizing catalysis demands navigation through complex and rugged fitness landscapes, with optimization trajectories often leading to strong diminishing returns and dead-ends. When no further improvements are observe…
  openalex Moshe Goldsmith, Dan S. Tawfik 2017-10-16 置信度 0.72 Enzyme kineticsCatalytic efficiencyCatalysisEnzymeYield (engineering)
• Enzyme Engineering for Nonaqueous Solvents: Random Mutagenesis to Enhance Activity of Subtilisin E in Polar Organic Media
  openalex Keqin Chen, Frances H. Arnold 1991-11-01 置信度 0.72 SubtilisinMutagenesisActive siteChemistryProtein engineering
• Improvement of enzyme activity, stability and selectivity via immobilization techniques
  openalex César Mateo, José M. Palomo, Gloria Fernández‐Lorente, José M. Guisán 等 2007-02-07 置信度 0.72 EnzymeImmobilized enzymeChemistrySelectivityEnzyme assay
• Multiplex Genome Engineering Using CRISPR/Cas Systems
  Functional elucidation of causal genetic variants and elements requires precise genome editing technologies. The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-…
  openalex Le Cong, F. Ann Ran, David Cox, Shuailiang Lin 等 2013-01-04 置信度 0.72 CRISPRCas9Genome editingGuide RNABiology
• Enzyme-catalyzed crosslinkable hydrogels: Emerging strategies for tissue engineering
  openalex Liliana Moreira Teixeira, Jan Feijén, Clemens van Blitterswijk, Pieter J. Dijkstra 等 2011-12-02 置信度 0.72 Self-healing hydrogelsMaterials scienceTissue engineeringSubstrate (aquarium)Catalysis
• Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis
  Glucosamine-6-phosphate N -acetyltransferase (GNA1) is the key enzyme that causes overproduction of N -acetylglucosamine in Bacillus subtilis . Previously, we increased GlcNAc production by promoting the expression of GNA1 from Caenorhabditis elegans ( Ce GNA1…
  openalex Wenlong Ma, Yanfeng Liu, Xueqin Lv, Jianghua Li 等 2019-01-04 置信度 0.72 Bacillus subtilisBiochemistryMetabolic engineeringEnzymeChemistry
• Enzyme Engineering by Targeted Libraries
  openalex Moshe Goldsmith, Dan S. Tawfik 2013-01-01 置信度 0.72 Computational biologyDirected evolutionPoint mutationProtein engineeringHigh-throughput screening
• Enzyme engineering for enantioselectivity: from trial-and-error to rational design?
  openalex Linda G. Otten, Frank Hollmann, Isabel W. C. E. Arends 2009-11-13 置信度 0.72 Rational designEnzymeProtein engineeringChemistryBiochemistry
• The new frontier of genome engineering with CRISPR-Cas9
  The advent of facile genome engineering using the bacterial RNA-guided CRISPR-Cas9 system in animals and plants is transforming biology. We review the history of CRISPR (clustered regularly interspaced palindromic repeat) biology from its initial discovery thr…
  openalex Jennifer A. Doudna, Emmanuelle Charpentier 2014-11-28 置信度 0.72 CRISPRCas9Genome editingBiologyComputational biology
• Rational engineering of enzyme stability
  openalex Vincent G. H. Eijsink, Alexandra Bjørk, Sigrid Gåseidnes, Reidun Sirevåg 等 2004-08-17 置信度 0.72 BarnaseRational designDirected evolutionProtein stabilityProtein engineering
• Unnatural amino acid mutagenesis-based enzyme engineering
  openalex Yuvaraj Ravikumar, Saravanan Prabhu Nadarajan, Tae Hyeon Yoo, Chong‐Soon Lee 等 2015-06-15 置信度 0.72 Protein engineeringAmino acidEnzymeDirected evolutionBiochemistry
• Enzyme engineering in the context of novel pathways and products
  openalex Konrad B. Otte, Bernhard Hauer 2015-01-10 置信度 0.72 Protein engineeringBiochemical engineeringContext (archaeology)Metabolic engineeringBiocatalysis
• Engineering enzyme access tunnels
  Enzymes are efficient and specific catalysts for many essential reactions in biotechnological and pharmaceutical industries. Many times, the natural enzymes do not display the catalytic efficiency, stability or specificity required for these industrial process…
  openalex Piia Kokkonen, David Bednář, Gaspar Pinto, Zbyněk Prokop 等 2019-04-23 置信度 0.72 Rational designCatalytic efficiencyBiochemical engineeringProtein engineeringEnzyme
• Synthesis of novel fructooligosaccharides by substrate and enzyme engineering
  openalex Rafael Beine, R. Moraru, Manfred Nimtz, Shukrallah Na’amnieh 等 2008-08-16 置信度 0.72 LevansucraseFructanChemistryPrebioticBiochemistry
• The impact of enzyme engineering upon natural product glycodiversification
  openalex Gavin J. Williams, Richard W. Gantt, Jon S. Thorson 2008-08-04 置信度 0.72 Natural productGlycosyltransferaseGlycosylProtein engineeringEnzyme
• Construction of a cellulase hyper-expression system in Trichoderma reesei by promoter and enzyme engineering
  BACKGROUND: Trichoderma reesei is the preferred organism for producing industrial cellulases. However, a more efficient heterologous expression system for enzymes from different organism is needed to further improve its cellulase mixture. The strong cbh1 promo…
  openalex Gen Zou, Shaohua Shi, Yan‐Ping Jiang, Joost van den Brink 等 2012-02-08 置信度 0.72 Trichoderma reeseiCellulaseEnzymeBiologyMicrobiology
• Characterization and engineering of a two-enzyme system for plastics depolymerization
  PETase depolymerizes PET, liberating soluble products, including mono(2-hydroxyethyl) terephthalate (MHET), which is cleaved to terephthalic acid and ethylene glycol by MHETase. Here, we report a 1.6 Å resolution MHETase structure, illustrating that the MHETas…
  openalex Brandon C. Knott, Erika Erickson, Mark D. Allen, Japheth E. Gado 等 2020-09-28 置信度 0.72 DepolymerizationEnzymeDeconstruction (building)MonomerCellulose
• Catalysis by a De Novo Zinc-Mediated Protein Interface: Implications for Natural Enzyme Evolution and Rational Enzyme Engineering
  Here we show that a recent computationally designed zinc-mediated protein interface is serendipitously capable of catalyzing carboxyester and phosphoester hydrolysis. Although the original motivation was to design a de novo zinc-mediated protein-protein intera…
  openalex Bryan S. Der, David Edwards, Brian Kuhlman 2012-04-17 置信度 0.72 Active siteZincProtein engineeringEnzymeChemistry
• Ultrahigh-Throughput Enzyme Engineering and Discovery in <i>In Vitro</i> Compartments
  variants a day with kHz frequencies. The repertoire of assays available for droplet screening covers all seven enzyme commission (EC) number classes, setting the stage for widespread use of droplet microfluidics in everyday biochemical experiments. We review t…
  openalex Maximilian Gantz, Stefanie Neun, Elliot J. Medcalf, Liisa van Vliet 等 2023-05-01 置信度 0.72 MicrofluidicsChemistryNanotechnologyHigh-throughput screeningInterfacing
• Multidisciplinary strategies to enhance therapeutic effects of flavonoids from Epimedii Folium: Integration of herbal medicine, enzyme engineering, and nanotechnology
  Flavonoids such as baohuoside I and icaritin are the major active compounds in Epimedii Folium (EF) and possess excellent therapeutic effects on various diseases. Encouragingly, in 2022, icaritin soft capsules were approved to reach the market for the treatmen…
  openalex Yi Lu, Qiulan Luo, Xiaobin Jia, James P. Tam 等 2022-12-30 置信度 0.72 EpimediumChemistryPharmacologyIn vivoTraditional Chinese medicine
• Review: Engineering of thermostable enzymes for industrial applications
  The catalytic properties of some selected enzymes have long been exploited to carry out efficient and cost-effective bioconversions in a multitude of research and industrial sectors, such as food, health, cosmetics, agriculture, chemistry, energy, and others. …
  openalex Federica Rigoldi, Stefano Donini, Alberto Redaelli, Emilio Parisini 等 2018-01-11 置信度 0.72 Biochemical engineeringProtein engineeringBiotechnologyAgricultureEnzyme
• Enzyme discovery and engineering for sustainable plastic recycling
  The drastically increasing amount of plastic waste is causing an environmental crisis that requires innovative technologies for recycling post-consumer plastics to achieve waste valorization while meeting environmental quality goals. Biocatalytic depolymerizat…
  openalex Baotong Zhu, Dong Wang, Na Wei 2021-03-03 置信度 0.72 Biochemical engineeringDepolymerizationBiotechnologyCatalytic efficiencyEnvironmental science
• Semi-rational approaches to engineering enzyme activity: combining the benefits of directed evolution and rational design
  openalex Roberto A. Chica, Nicolas Doucet, Joelle N. Pelletier 2005-07-02 置信度 0.72 Rational designDirected evolutionProtein engineeringDirected Molecular EvolutionMutagenesis
• Structure‐Based Enzyme Engineering and Its Impact on In Vitro Glycorandomization
  Glycoengineering: This review highlights the application of structure-based enzyme engineering toward enhancing the promiscuity of nucleotidylytransferases (such as Ep, whose active site is shown here) and glycosyltransferases, which are essential to the in vi…
  openalex Jon S. Thorson, William A. Barton, Dirk Hoffmeister, Christoph Albermann 等 2003-12-19 置信度 0.72 GlycosyltransferaseIn vitroEnzymeBiochemistryProtein engineering
• From Protein Engineering to Immobilization: Promising Strategies for the Upgrade of Industrial Enzymes
  Enzymes found in nature have been exploited in industry due to their inherent catalytic properties in complex chemical processes under mild experimental and environmental conditions. The desired industrial goal is often difficult to achieve using the native fo…
  openalex Raushan Kumar Singh, Manish Kumar Tiwari, Ranjitha Singh, Jung-Kul Lee 2013-01-10 置信度 0.72 Protein engineeringEnzymeImmobilized enzymeBiochemical engineeringChemistry
• Enzyme Engineering, Future Directions
  I. General Problems of Enzyme Engineering.- Stabilization and Reactivation of Enzymes.- Theory of Reliability and Kinetics of Inactivation of Biocatalytical Systems.- II. Carbohydrate Conversions with Enzymes.- Enzymatic Conversion of Cellulose to Glucose: Pre…
  openalex Lemuel B. Wingard, И.В. Березин, A. A. Klysov, David Gough 1981-08-01 置信度 0.72 EnzymeImmobilized enzymeChemistryStarchBiochemistry
• Enzyme engineering in biomimetic compartments
  openalex Pierre-Yves Colin, Anastasia Zinchenko, Florian Hollfelder 2015-08-01 置信度 0.72 Directed evolutionComputer scienceNanotechnologyBiochemical engineeringCompartment (ship)
• Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering
  Abstract 5-Hydroxymethylfurfural (HMF) has emerged as a crucial bio-based chemical building block in the drive towards developing materials from renewable resources, due to its direct preparation from sugars and its readily diversifiable scaffold. A key obstac…
  openalex William R. Birmingham, Asbjørn Toftgaard Pedersen, Mafalda Dias Gomes, Mathias Bøje Madsen 等 2021-08-16 置信度 0.72 BiocatalysisChemistryHydroxymethylfurfural5-hydroxymethylfurfuralMetabolic engineering
• High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering
  evolution campaigns include improved folding stability, catalytic activity, and/or substrate specificity. Despite significant progress in recent years in the areas of high-throughput screening and DNA sequencing, our ability to explore the vast space of functi…
  openalex Rosario Vanella, Gordana Kovačević, Vanni Doffini, Jaime Fernández de Santaella 等 2022-01-01 置信度 0.72 ThroughputHigh-throughput screeningComputer scienceDNA sequencingComputational biology
• Efficient production of lycopene in Saccharomyces cerevisiae by enzyme engineering and increasing membrane flexibility and NAPDH production
  openalex Juhyun Hong, Seong-Hee Park, Sujin Kim, Seon-Won Kim 等 2018-10-20 置信度 0.72 LycopeneSaccharomyces cerevisiaeBiochemistryMetabolic engineeringBiology
• The Generation and Exploitation of Protein Mutability Landscapes for Enzyme Engineering
  The increasing number of enzyme applications in chemical synthesis calls for new engineering methods to develop the biocatalysts of the future. An interesting concept in enzyme engineering is the generation of large-scale mutational data in order to chart prot…
  openalex Jan‐Ytzen van der Meer, Lieuwe Biewenga, Gerrit J. Poelarends 2016-07-21 置信度 0.72 Protein engineeringDirected evolutionMutagenesisComputational biologyDirected Molecular Evolution
• Accelerating Biocatalysis Discovery with Machine Learning: A Paradigm Shift in Enzyme Engineering, Discovery, and Design
  Emerging computational tools promise to revolutionize protein engineering for biocatalytic applications and accelerate the development timelines previously needed to optimize an enzyme to its more efficient variant. For over a decade, the benefits of predictiv…
  openalex Braun Markus, Gruber Christian C, Krassnigg Andreas, Kummer Arkadij 等 2023-10-26 置信度 0.72 Computer scienceIdentification (biology)Field (mathematics)TimelineData science
• Engineering the spatial organization of metabolic enzymes: mimicking nature's synergy
  openalex Robert Conrado, Jeffrey D. Varner, Matthew P. DeLisa 2008-09-07 置信度 0.72 EnzymeMetabolic pathwayMetabolic engineeringBiologyBiochemistry
• Suicide Inactivation of Peroxidases and the Challenge of Engineering More Robust Enzymes
  openalex Brenda Valderrama, Marcela Ayala, Rafael Vázquez-Duhalt 2002-05-01 置信度 0.72 PeroxidaseEnzymeBiologyBiochemical engineeringBiochemistry
• Synthetic metabolism: metabolic engineering meets enzyme design
  Metabolic engineering aims at modifying the endogenous metabolic network of an organism to harness it for a useful biotechnological task, for example, production of a value-added compound. Several levels of metabolic engineering can be defined and are the topi…
  openalex Tobias J. Erb, Patrik R. Jones, Arren Bar‐Even 2017-01-30 置信度 0.72 Metabolic engineeringSynthetic biologyMetabolic pathwayMetabolic networkBiochemical engineering
• Improvement of a synthetic live bacterial therapeutic for phenylketonuria with biosensor-enabled enzyme engineering
  In phenylketonuria (PKU) patients, a genetic defect in the enzyme phenylalanine hydroxylase (PAH) leads to elevated systemic phenylalanine (Phe), which can result in severe neurological impairment. As a treatment for PKU, Escherichia coli Nissle (EcN) strain S…
  openalex Kristin J. Adolfsen, Isolde Callihan, Catherine Monahan, Per Greisen 等 2021-10-28 置信度 0.72 Phenylalanine hydroxylasePhenylalanineEnzymePhenylalanine ammonia-lyaseDeamination
• Modern computational methods for rational enzyme engineering
  openalex Pedro Ferreira, Pedro Alexandrino Fernandes, Maria J. Ramos 2022-10-01 置信度 0.72 Biochemical engineeringThrivingProtein engineeringValue (mathematics)Field (mathematics)
• Rational Enzyme Engineering Through Biophysical and Biochemical Modeling
  Due to its importance in the pharmaceutical industry, ligand dynamic simulations have experienced a great expansion. Using all-atom models and cutting-edge hardware, one can perform nonbiased ligand migration, active site search, and binding studies. In this L…
  openalex Sandra Acebes, Elena Fernández‐Fueyo, Emanuele Monza, Maria Fátima Lucas 等 2016-02-03 置信度 0.72 MutagenesisActive siteLigand (biochemistry)Rational designProtein engineering
• Engineering an enzyme to resist boiling
  In recent years, many efforts have been made to isolate enzymes from extremophilic organisms in the hope to unravel the structural basis for hyperstability and to obtain hyperstable biocatalysts. Here we show how a moderately stable enzyme (a thermolysin-like …
  openalex Bertus van den Burg, Gert Vriend, Oene R. Veltman, Gerard Venema 等 1998-03-03 置信度 0.72 MutantThermolysinEnzymeBiochemistryProtease
• Rational Protein Design of ThDP‐Dependent Enzymes—Engineering Stereoselectivity
  Benzoylformate decarboxylase (BFD) from Pseudomonas putida is an exceptional thiamin diphosphate-dependent enzyme, as it catalyzes the formation of (S)-2-hydroxy-1-phenylpropan-1-one from benzaldehyde and acetaldehyde. This is the only currently known S-select…
  openalex Dörte Gocke, Lydia Walter, E.V. Gauchenova, Geraldine Kolter 等 2008-01-25 置信度 0.72 StereoselectivityChemistrySteric effectsStereochemistryBenzaldehyde
• Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts
  openalex Claudia Bernal, Karen Rodríguez, Ronny Martínez 2018-06-09 置信度 0.72 Biochemical engineeringProtein engineeringImmobilized enzymeBioprocessBiocatalysis
• Revolutionizing enzyme engineering through artificial intelligence and machine learning
  The combinatorial space of an enzyme sequence has astronomical possibilities and exploring it with contemporary experimental techniques is arduous and often ineffective. Multi-target objectives such as concomitantly achieving improved selectivity, solubility a…
  openalex Nitu Singh, Sunny Malik, Anvita Gupta, Kinshuk Raj Srivastava 2021-04-09 置信度 0.72 Directed evolutionArtificial intelligenceProtein engineeringScope (computer science)Computer science
• Enzyme engineering for biocatalysis
  Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type enzymes often hi…
  openalex Caroline E. Paul, Ulf Hanefeld, Frank Hollmann, Ge Qu 等 2024-01-31 置信度 0.72 BiocatalysisEnzymeChemistryBiochemical engineeringCatalysis
• Pathway enzyme engineering for flavonoid production in recombinant microbes
  Metabolic engineering of microbial strains for the production of flavonoids of industrial interest has attracted great attention due to its promising advantages over traditional extraction approaches, such as independence of plantation, facile downstream separ…
  openalex Jian Zha, Xia Wu, Guoli Gong, Mattheos Koffas 2019-10-17 置信度 0.72 Metabolic engineeringBiotechnologyBiochemical engineeringFlavonoidProduction (economics)
• Biosynthesis of fatty acid-derived hydrocarbons: perspectives on enzymology and enzyme engineering
  openalex Kun Liu, Shengying Li 2019-09-17 置信度 0.72 BiofuelRenewable energyBiochemical engineeringFossil fuelRenewable fuels
• Deconstructing PET: Advances in enzyme engineering for sustainable plastic degradation
  openalex Jiaxin Yao, Yao Liu, Zhenghua Gu, Liang Zhang 等 2024-07-21 置信度 0.72 Degradation (telecommunications)ChemistryMaterials scienceBiochemical engineeringEngineering
• Enzyme engineering for biosynthetic cascades
  openalex John A. McIntosh, Andrew E. Owens 2021-01-30 置信度 0.72 Synthetic biologyProtein engineeringBiochemical engineeringTransformative learningMetabolic engineering
• The Thiamine diphosphate dependent Enzyme Engineering Database: A tool for the systematic analysis of sequence and structure relations
  BACKGROUND: Thiamine diphosphate (ThDP)-dependent enzymes form a vast and diverse class of proteins, catalyzing a wide variety of enzymatic reactions including the formation or cleavage of carbon-sulfur, carbon-oxygen, carbon-nitrogen, and especially carbon-ca…
  openalex Michael Widmann, Robert Radloff, Jürgen Pleiss 2010-02-01 置信度 0.72 Thiamine pyrophosphateBiochemistryEnzymeThiamineSequence alignment
• Redefining Biology via Enzyme Engineering
  openalex Mirna Kvajo 2017-01-01 置信度 0.72 SiliconOrganosiliconNanotechnologySynthetic biologyBiology
• Therapeutic enzyme engineering using a generative neural network
  Enhancing the potency of mRNA therapeutics is an important objective for treating rare diseases, since it may enable lower and less-frequent dosing. Enzyme engineering can increase potency of mRNA therapeutics by improving the expression, half-life, and cataly…
  openalex Andrew J. Giessel, Athanasios Dousis, Kanchana Ravichandran, Kevin Smith 等 2022-01-27 置信度 0.72 Urea cyclePotencyEnzymeComputational biologySequence (biology)
• Engineering the third wave of biocatalysis
  openalex Uwe T. Bornscheuer, Gjalt W. Huisman, Romas J. Kazlauskas, Stefan Lutz 等 2012-05-01 置信度 0.72 BiocatalysisCommodity chemicalsBiochemical engineeringSynthetic biologyProtein engineering
• Engineering of Flexible Loops in Enzymes
  ADVERTISEMENT RETURN TO ISSUEPREVViewpointNEXTEngineering of Flexible Loops in EnzymesBettina M. Nestl and Bernhard Hauer*View Author Information Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany*E-mail: [em…
  openalex Bettina M. Nestl, Bernhard Hauer 2014-08-08 置信度 0.72 CitationAltmetricsComputer scienceSocial mediaLibrary science
• Rational modification of enzyme catalysis by engineering surface charge
  openalex Alan J. Russell, Alan R. Fersht 1987-08-01 置信度 0.72 SubtilisinCatalysisEnzymeMutagenesisSurface charge
• Chassis and key enzymes engineering for monoterpenes production
  openalex Lu Zhang, Wenhai Xiao, Ying Wang, Mingdong Yao 等 2017-09-07 置信度 0.72 ChassisCytotoxicityChemistryMetabolic engineeringBiochemical engineering
• Directed Evolution Methods for Enzyme Engineering
  Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-lab methods…
  openalex Saurabh Nirantar 2021-09-15 置信度 0.72 Biochemical engineeringVariety (cybernetics)Directed evolutionEnzymeBiotechnology
• Enzyme Engineering
  Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deli…
  openalex Manfred T. Reetz, Zhoutong Sun, Ge Qu 2023-01-06 置信度 0.72 Chemistry
• Spatial organization of enzymes for metabolic engineering
  openalex Hanson Lee, William C. DeLoache, John E. Dueber 2011-09-18 置信度 0.72 EnzymeFlux (metallurgy)Compartmentalization (fire protection)Metabolic pathwaySynthetic biology
• Computational tools for designing and engineering enzymes
  openalex Jiřı́ Damborský, Jan Brezovský 2014-01-03 置信度 0.72 In silicoComputational biologyComplement (music)Protein engineeringComputer science
• Engineering precision nanoparticles for drug delivery
  openalex Michael J. Mitchell, Margaret M. Billingsley, Rebecca M. Haley, Marissa E. Wechsler 等 2020-12-04 置信度 0.72 Precision medicineDrug deliveryPersonalized medicineNanotechnologyComputer science
• Engineering enzymes for noncanonical amino acid synthesis
  The standard proteinogenic amino acids grant access to a myriad of chemistries that harmonize to create life. Outside of these twenty canonical protein building blocks are countless noncanonical amino acids (ncAAs), either found in nature or created by man. In…
  openalex Patrick J. Almhjell, Christina E. Boville, Frances H. Arnold 2018-01-01 置信度 0.72 EnzymeChemistryAmino acidBiochemistry
• Engineering an enzyme by site-directed mutagenesis to be resistant to chemical oxidation.
  Site-directed mutagenesis can be employed to alter activity critical residues in proteins which are susceptible to chemical oxidation. Previous studies have implicated methionine 222 as a primary site for oxidative inactivation of subtilisin (Stauffer, C. E., …
  openalex David A. Estell, Thomas P. Graycar, James A. Wells 1985-06-01 置信度 0.72 SubtilisinMutagenesisSite-directed mutagenesisBiochemistryChemistry
• Engineering Enzyme Specificity by "Substrate-Assisted Catalysis"
  A novel approach to engineering enzyme specificity is presented in which a catalytic group from an enzyme is first removed by site-directed mutagenesis causing inactivation. Activity is then partially restored by substrates containing the missing catalytic fun…
  openalex Paul Carter, James A. Wells 1987-07-24 置信度 0.72 SubtilisinCatalytic triadSerine proteaseChemistrySubstrate (aquarium)
• Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications
  Cytochrome P450 enzymes (P450s) are broadly distributed among living organisms and play crucial roles in natural product biosynthesis, degradation of xenobiotics, steroid biosynthesis, and drug metabolism. P450s are considered as the most versatile biocatalyst…
  openalex Zhong Li, Yuanyuan Jiang, F. Peter Guengerich, Li Ma 等 2020-01-01 置信度 0.72 Cytochrome P450Protein engineeringXenobioticBioremediationSubstrate (aquarium)
• Enzyme engineering reaches the boiling point
  Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans the biological, physical, and social sciences.
  openalex Frances H. Arnold 1998-03-03 置信度 0.72 Biological sciencesLibrary scienceEngineering ethicsEngineering physicsBiology
• Enhanced production of polyunsaturated fatty acids by enzyme engineering of tandem acyl carrier proteins
  Abstract In some microorganisms, polyunsaturated fatty acids (PUFAs) are biosynthesized by PUFA synthases characterized by tandem acyl carrier proteins (ACPs) in subunit A. These ACPs were previously shown to be important for PUFA productivity. In this study, …
  openalex Shohei Hayashi, Yasuharu Satoh, Tetsuro Ujihara, Yusuke Takata 等 2016-10-18 置信度 0.72 Polyunsaturated fatty acidEnzymeProtein subunitBiochemistryBiology
• Structure and Cooperativity in Substrate–Enzyme Interactions: Perspectives on Enzyme Engineering and Inhibitor Design
  Enzyme-based synthetic chemistry provides a green way to synthesize industrially important chemical scaffolds and provides incomparable substrate specificity and unmatched stereo-, regio-, and chemoselective product formation. However, using biocatalysts at an…
  openalex Eerappa Rajakumara, Suman Abhishek, Kulhar Nitin, Dubey Saniya 等 2022-01-18 置信度 0.72 CooperativityEnzymeSubstrate (aquarium)Protein engineeringEnzyme inhibition
• Proteolytic Enzyme Engineering: A Tool for Wool
  One of the goals of protein engineering is to tailor the structure of enzymes to optimize industrial bioprocesses. In the present work, we present the construction of a novel high molecular weight subtilisin, based on the fusion of the DNA sequences coding for…
  openalex Rita Araújo, Carla Silva, Raúl Machado, Margarida Casal 等 2009-05-21 置信度 0.72 WoolBacillus subtilisProteaseChemistryProteases
• Strategies for the discovery and engineering of enzymes for biocatalysis
  openalex Timo Davids, Marlen Schmidt, Dominique Böttcher, Uwe T. Bornscheuer 2013-03-21 置信度 0.72 Directed evolutionProtein engineeringBiocatalysisBiochemical engineeringDirected Molecular Evolution
• Fundamentals of Enzyme Engineering
  openalex Young Je Yoo, Yan Feng, Yong Hwan Kim, Camila Flor J. Yagonia 2017-01-01 置信度 0.72 EnzymeChemistryBiochemistry
• Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration
  Enzymes need to be efficient, robust, and highly specific for their effective use in commercial bioproduction. These properties can be introduced using various enzyme engineering techniques, with random mutagenesis and directed evolution (DE) often being chose…
  openalex Jochem R Nielsen, Ruud A. Weusthuis, Wei E. Huang 2023-01-18 置信度 0.72 CofactorNAD+ kinaseEnzymeRedoxBiochemistry
• Cell-free systems for accelerating glycoprotein expression and biomanufacturing
  Protein glycosylation, the enzymatic modification of amino acid sidechains with sugar moieties, plays critical roles in cellular function, human health, and biotechnology. However, studying and producing defined glycoproteins remains challenging. Cell-free gly…
  openalex Jasmine M. Hershewe, Weston Kightlinger, Michael C. Jewett 2020-10-22 置信度 0.72 BiomanufacturingGlycoproteinGlycosylationSynthetic biologyBiochemistry
• Building biomanufacturing capacity—the chapter and verse
  openalex Michael E. Kamarck, Michael E Kamarck 2006-05-01 置信度 0.72 BiomanufacturingBiopharmaceuticalBusinessManufacturing engineeringEngineering
• Biomanufacturing of Therapeutic Cells: State of the Art, Current Challenges, and Future Perspectives
  Stem cells and other functionally defined therapeutic cells (e.g., T cells) are promising to bring hope of a permanent cure for diseases and disorders that currently cannot be cured by conventional drugs or biological molecules. This paradigm shift in modern m…
  openalex Kyung‐Ho Roh, Robert M. Nerem, Krishnendu Roy 2016-06-07 置信度 0.72 BiomanufacturingIsolation (microbiology)Stem cellBiotechnologyNanotechnology
• Sustained regeneration of high-volume adipose tissue for breast reconstruction using computer aided design and biomanufacturing
  openalex Mohit P. Chhaya, Ferry P.W. Melchels, Boris Michael Holzapfel, Jeremy Baldwin 等 2015-03-18 置信度 0.72 Adipose tissueBiomedical engineeringTissue engineeringScaffoldRegeneration (biology)
• Stoichiometric Conversion of Cellulosic Biomass by in Vitro Synthetic Enzymatic Biosystems for Biomanufacturing
  Cellulosic biomass is the earth’s most abundant renewable resource, which is considered to be a promising feedstock for manufacturing biofuels and biochemicals. In this study, stoichiometric enzymatic phosphorolysis of cellulosic biomass for manufacturing bioc…
  openalex Dongdong Meng, Xinlei Wei, Y.‐H. Percival Zhang, Zhiguang Zhu 等 2018-09-05 置信度 0.72 Cellulosic ethanolChemistryEnzymatic hydrolysisCellobioseBiomass (ecology)
• Practical monitoring technologies for cells and substrates in biomanufacturing
  openalex Kenneth F. Reardon 2021-09-02 置信度 0.72 BiomanufacturingBioprocessBioreactorAutomationProcess engineering
• Advancing Yarrowia lipolytica as a superior biomanufacturing platform by tuning gene expression using promoter engineering
  openalex Mei-Li Sun, Tian-Qiong Shi, Lu Lin, Rodrigo Ledesma-Amaro 等 2022-01-11 置信度 0.72 YarrowiaBiomanufacturingPromoterYeastSynthetic biology
• Peroxisome-based metabolic engineering for biomanufacturing and agriculture
  openalex Shuyan Song, Cuifang Ye, Yijun Jin, Huaxin Dai 等 2024-02-28 置信度 0.72 BiomanufacturingMetabolic engineeringAgricultureBiotechnologyBiochemical engineering
• Rapid model-guided design of organ-scale synthetic vasculature for biomanufacturing
  Our ability to produce human-scale bio-manufactured organs is critically limited by the need for vascularization and perfusion. For tissues of variable size and shape, including arbitrarily complex geometries, designing and printing vasculature capable of adeq…
  openalex Zachary A. Sexton, Dominic Rütsche, Jessica E. Herrmann, Andrew R. Hudson 等 2025-06-12 置信度 0.72 BiomanufacturingScale (ratio)Computational biologyComputer scienceBiology
• Perspectives for Using CO2 as a Feedstock for Biomanufacturing of Fuels and Chemicals
  Microbial cell factories offer an eco-friendly alternative for transforming raw materials into commercially valuable products because of their reduced carbon impact compared to conventional industrial procedures. These systems often depend on lignocellulosic f…
  openalex Elif Kurt, Jiansong Qin, Alexandria Williams, Youbo Zhao 等 2023-11-26 置信度 0.72 BiomanufacturingRaw materialBioprocessBiochemical engineeringGreenhouse gas
• Multi-fidelity Gaussian Process for Biomanufacturing Process Modeling with Small Data
  In biomanufacturing, developing an accurate model to simulate the complex dynamics of bioprocesses is an important yet challenging task. This is partially due to the uncertainty associated with bioprocesses, high data acquisition cost, and lack of data availab…
  arxiv Yuan Sun, Winton Nathan-Roberts, Tien Dung Pham, Ellen Otte 等 2022-11-26T06:38:34Z 置信度 0.78 cs.LGcs.AI
• Quantum enhanced ensemble GANs for anomaly detection in continuous biomanufacturing
  The development of continuous biomanufacturing processes requires robust and early anomaly detection, since even minor deviations can compromise yield and stability, leading to disruptions in scheduling, reduced weekly production, and diminished economic perfo…
  arxiv Rajiv Kailasanathan, William R. Clements, Mohammad Reza Boskabadi, Shawn M. Gibford 等 2025-08-29T09:05:56Z 置信度 0.78 cs.LGq-bio.OTquant-ph
• RAPTOR-GEN: RApid PosTeriOR GENerator for Bayesian Learning in Biomanufacturing
  Biopharmaceutical manufacturing is vital to public health but lacks the agility for rapid, on-demand production of biotherapeutics due to the complexity and variability of bioprocesses. To overcome this, we introduce RApid PosTeriOR GENerator (RAPTOR-GEN), a m…
  arxiv Wandi Xu, Wei Xie 2025-09-25T05:20:49Z 置信度 0.78 stat.MLcs.LG
• From Discovery to Production: Challenges and Novel Methodologies for Next Generation Biomanufacturing
  The increasingly pressing demand of novel drugs (e.g., gene therapies for personalized cancer care, ever evolving vaccines) with unprecedented levels of personalization, has put a remarkable pressure on the traditionally long time required by the pharma R&amp;…
  arxiv Wei Xie, Giulia Pedrielli 2022-05-08T17:32:17Z 置信度 0.78 q-bio.QMeess.SY
• Policy Optimization in Dynamic Bayesian Network Hybrid Models of Biomanufacturing Processes
  Biopharmaceutical manufacturing is a rapidly growing industry with impact in virtually all branches of medicines. Biomanufacturing processes require close monitoring and control, in the presence of complex bioprocess dynamics with many interdependent factors, …
  arxiv Hua Zheng, Wei Xie, Ilya O. Ryzhov, Dongming Xie 2021-05-13T20:39:02Z 置信度 0.78 cs.AIcs.LG
• Interpretable Biomanufacturing Process Risk and Sensitivity Analyses for Quality-by-Design and Stability Control
  While biomanufacturing plays a significant role in supporting the economy and ensuring public health, it faces critical challenges, including complexity, high variability, lengthy lead time, and very limited process data, especially for personalized new cell a…
  arxiv Wei Xie, Bo Wang, Cheng Li, Dongming Xie 等 2019-09-10T03:26:01Z 置信度 0.78 stat.MLcs.LGeess.SY
• Sensitivity Analysis on Interaction Effects of Policy-Augmented Bayesian Networks
  Biomanufacturing plays an important role in supporting public health and the growth of the bioeconomy. Modeling and studying the interaction effects among various input variables is very critical for obtaining a scientific understanding and process specificati…
  arxiv Junkai Zhao, Jun Luo, Wei Xie, Zixuan Bai 2024-11-23T13:47:35Z 置信度 0.78 stat.MEstat.CO
• Sensitivity Analysis on Policy-Augmented Graphical Hybrid Models with Shapley Value Estimation
  Driven by the critical challenges in biomanufacturing, including high complexity and high uncertainty, we propose a comprehensive and computationally efficient sensitivity analysis framework for general nonlinear policy-augmented knowledge graphical (pKG) hybr…
  arxiv Junkai Zhao, Wei Xie, Jun Luo 2024-11-20T15:33:39Z 置信度 0.78 stat.MLstat.CO
• Biomanufacturing Harvest Optimization with Small Data
  In biopharmaceutical manufacturing, fermentation processes play a critical role in productivity and profit. A fermentation process uses living cells with complex biological mechanisms, leading to high variability in the process outputs, namely, the protein and…
  arxiv Bo Wang, Wei Xie, Tugce Martagan, Alp Akcay 等 2021-01-11T07:47:25Z 置信度 0.78 stat.MLcs.LG