基因技术工具 · 全部实体（第 89 / 100 页）

本页收录第 8801–8900 条，共 10000 条 · 返回 基因技术工具 首页

• Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals
  Humans are sorely over-dependent on livestock for their daily basic need of food in the form of meat, milk, and eggs. Therefore, genetic engineering and transgenesis provide the opportunity for more significant gains and production in a short span of time. One…
  semanticscholar Parul Singh, S. Ali 2021-06-30 置信度 0.74 Veterinary Sciences
• Genome Engineering Using the CRISPR Cas9 System
  semanticscholar A. Ibrahim, Mehmet Özsöz, Z. Saeed, Galaya Tirah 等 2019-06-17 置信度 0.74
• A compact Cascade-Cas3 system for targeted genome engineering
  CRISPR–Cas technologies have enabled programmable gene editing in eukaryotes and prokaryotes. However, the leading Cas9 and Cas12a enzymes are limited in their ability to make large deletions. Here, we used the processive nuclease Cas3, together with a minimal…
  semanticscholar Bálint Csörgő, Lina M. Leon, Ilea J. Chau-Ly, Alejandro Vasquez-Rifo 等 2020-10-19 置信度 0.74 Nature Methods
• Read Online Genome Engineering Using The Crispr Cas9 System Mit
  semanticscholar 2021 置信度 0.74
• Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering
  Traditionally, generation of new plants with improved or desirable features has relied on laborious and time-consuming breeding techniques. Genome-editing technologies have led to a new era of genome engineering, enabling an effective, precise, and rapid engin…
  semanticscholar Naoki Wada, Risa Ueta, Yuriko Osakabe, K. Osakabe 2020-05-25 置信度 0.74 BMC Plant Biology
• Multiplexed genome engineering by Cas12a and CRISPR arrays encoded on single transcripts
  semanticscholar C. Campa, Niels R Weisbach, Antonio J. Santinha, Danny Incarnato 等 2019-08-12 置信度 0.74 Nature Methods
• CRISPR–Cas12b enables efficient plant genome engineering
  semanticscholar Meiling Ming, Qiurong Ren, Changtian Pan, Yao He 等 2020-03-01 置信度 0.74 Nature Plants
• Find and cut-and-transfer (FiCAT) mammalian genome engineering
  While multiple technologies for small allele genome editing exist, robust technologies for targeted integration of large DNA fragments in mammalian genomes are still missing. Here we develop a gene delivery tool (FiCAT) combining the precision of a CRISPR-Cas9…
  semanticscholar Maria Pallarès-Masmitjà, Dimitrije Ivančić, Júlia Mir-Pedrol, Jessica Jaraba-Wallace 等 2021-12-01 置信度 0.74 Nature Communications
• Synthetic control of plasmid replication enables target- and self-curing of vectors and expedites genome engineering of Pseudomonas putida
  Genome engineering of non-conventional microorganisms calls for the development of dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic soil bacterium widely used for metabolic engineering owing to its versatile metabolism a…
  semanticscholar D. Volke, Laura Friis, Nicolas T. Wirth, Justine Turlin 等 2020-03-19 置信度 0.74 Metabolic Engineering Communications
• Universal toxin-based selection for precise genome engineering in human cells
  Prokaryotic restriction enzymes, recombinases and Cas proteins are powerful DNA engineering and genome editing tools. However, in many primary cell types, the efficiency of genome editing remains low, impeding the development of gene- and cell-based therapeuti…
  semanticscholar Songyuan Li, N. Akrap, S. Cerboni, M. Porritt 等 2021-01-21 置信度 0.74 Nature Communications
• CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering
  Existing technologies for site-specific integration of kilobase-sized DNA sequences in bacteria are limited by low efficiency, a reliance on recombination, the need for multiple vectors, and challenges in multiplexing. To address these shortcomings, we introdu…
  semanticscholar P. Vo, Carlotta Ronda, Sanne E. Klompe, Ethan E. Chen 等 2020-11-23 置信度 0.74 Nature Biotechnology
• Plant Genome Engineering for Targeted Improvement of Crop Traits
  To improve food security, plant biology research aims to improve crop yield and tolerance to biotic and abiotic stress, as well as increasing the nutrient contents of food. Conventional breeding systems have allowed breeders to produce improved varieties of ma…
  semanticscholar Khalid Sedeek, Ahmed Mahas, Magdy M. Mahfouz 2019-02-12 置信度 0.74 Frontiers in Plant Science
• Precise genome engineering in Drosophila using prime editing
  Significance Precise genome engineering allows researchers to modify gene function, tag endogenous proteins, or model human disease mutations. Here, we adapt prime editing, a new CRISPR-based technology that uses reverse transcription to write precise changes …
  semanticscholar Justin A. Bosch, Gabriel Birchak, N. Perrimon 2020-08-05 置信度 0.74 Proceedings of the National Academy of Sciences of the United States of America
• Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes
  semanticscholar Chun Wang, Qing Liu, Yi Shen, Yufeng Hua 等 2019-01-04 置信度 0.74 Nature Biotechnology
• Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice
  Precise genome editing by systems such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) requires high-efficiency homology-directed repair (HDR). Different technologies have been developed to improve HDR b…
  semanticscholar Zahir Ali, A. Shami, Khalid Sedeek, Radwa Kamel 等 2020-01-23 置信度 0.74 Communications Biology
• Characterization and repurposing of the endogenous Type I-F CRISPR–Cas system of Zymomonas mobilis for genome engineering
  Establishment of production platform organisms through prokaryotic engineering represents an efficient means to generate alternatives for yielding renewable biochemicals and biofuels from sustainable resources. Zymomonas mobilis, a natural facultative anaerobi…
  semanticscholar Yanli Zheng, Jiamei Han, Wenyao Liang, Runxia Li 等 2019-03-12 置信度 0.74 bioRxiv
• Enhancing phage therapy through synthetic biology and genome engineering
  The antimicrobial and therapeutic efficacy of bacteriophages is currently limited, mostly due to rapid emergence of phage-resistance and the inability of most phage isolates to bind and infect a broad range of clinical strains. Here, we discuss how phage thera…
  semanticscholar B. R. Lenneman, Jonas Fernbach, M. Loessner, T. Lu 等 2020-12-10 置信度 0.74 Current Opinion in Biotechnology
• Accelerated genome engineering of Pseudomonas putida by I‐SceI―mediated recombination and CRISPR‐Cas9 counterselection
  Pseudomonas species have become reliable platforms for bioproduction due to their capability to tolerate harsh conditions imposed by large‐scale bioprocesses and their remarkable resistance to diverse physicochemical stresses. The last few years have brought f…
  semanticscholar Nicolas T. Wirth, Ekaterina Kozaeva, P. Nikel 2019-03-12 置信度 0.74 Microbial Biotechnology
• Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins
  Natural killer (NK) cells are an attractive cell-type for adoptive immunotherapy, but challenges in preparation of therapeutic primary NK cells restrict patient accessibility to NK cell immunotherapy. NK-92 is a well-characterized human NK cell line that has d…
  semanticscholar Rih-Sheng Huang, Hsin-An Shih, Min-Chi Lai, Yao-Jen Chang 等 2020-05-22 置信度 0.74 Frontiers in Immunology
• Precision genome engineering in rice using prime editing system
  Making precise changes in the genomes of organisms is challenging for most genome editing tools. Recently, a search-and-replace method, also known as prime editing, was developed that can introduce user-defined sequence into a target site without requiring dou…
  semanticscholar Kai Hua, Yuwei Jiang, Xiaoping Tao, Jian‐Kang Zhu 2020-05-06 置信度 0.74 Plant Biotechnology Journal
• One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas mediated genome engineering
  SUMMARY The type II bacterial CRISPR/Cas system is a novel genome engineering technology with the ease of multiplexed gene targeting. Here we created reporter and conditional mutant mice by co-injection of zygotes with Cas9 mRNA, different guide RNAs (sgRNAs) …
  semanticscholar Hui Yang, Haoyi Wang, C. Shivalila, A. Cheng 等 2013-08-01 置信度 0.74 Cell
• Harnessing type I CRISPR–Cas systems for genome engineering in human cells
  semanticscholar P. Cameron, M. Coons, Sanne E. Klompe, Alexandra M. Lied 等 2019-11-18 置信度 0.74 Nature Biotechnology
• Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering.
  Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed, with…
  semanticscholar Yu Wang, Ye Liu, Ping Zheng, Jibin Sun 等 2020-07-14 置信度 0.74 Trends in Biotechnology
• Pooled Knock-In Targeting for Genome Engineering of Cellular Immunotherapies
  SUMMARY Adoptive transfer of genetically-modified immune cells holds great promise for cancer immunotherapy. CRISPR knock-in targeting can improve cell therapies, but more high-throughput methods are needed to test which knock-in gene constructs most potently …
  semanticscholar Theodore L. Roth, P. J. Li, Franziska Blaeschke, Jasper F. Nies 等 2020-04-01 置信度 0.74 Cell
• Plug and Play Protein Modification using Homology-independent Universal Genome Engineering
  Summary Analysis of endogenous protein localization, function, and dynamics is fundamental to the study of all cells, including the diversity of cell types in the brain. However, current approaches are often low-throughput and resource-intensive. Here we descr…
  semanticscholar Yudong Gao, Erin Hisey, T. Bradshaw, E. Erata 等 2019-07-01 置信度 0.74 Neuron
• Porcine genome engineering for xenotransplantation.
  The extreme shortage of human donor organs for treatment of patients with end-stage organ failures is well known. Xenotransplantation, which might provide unlimited organ supply, is a most promising strategy to solve this problem. Domestic pigs are regarded as…
  semanticscholar Dong Niu, Xiang Ma, Taoyan Yuan, Yifan Niu 等 2020-04-07 置信度 0.74 Advanced Drug Delivery Reviews
• A Highly Efficient CRISPR-Cas9-Based Genome Engineering Platform in Acinetobacter baumannii to Understand the H2O2-Sensing Mechanism of OxyR.
  The rapid emergence of extensively drug-resistant A. baumannii has posed a major threat to global public health, emphasizing the desperate need for novel therapeutic strategies. We report the development of a highly efficient genome-engineering platform in A. …
  semanticscholar Yu Wang, Zhipeng Wang, Yan Chen, X. Hua 等 2019-12-01 置信度 0.74 Cell Chemical Biology
• Extensive germline genome engineering in pigs
  semanticscholar Y. Yue, Weihong Xu, Yinan Kan, Hong-Ye Zhao 等 2020-09-21 置信度 0.74 Nature Biomedical Engineering
• Genome Engineering in Rice Using Cas9 Variants that Recognize NG PAM Sequences.
  CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif (PAM) requirement hinders applications of the CRISPR/Cas9 system since it restricts the target…
  semanticscholar Kai Hua, Xiaoping Tao, Peijin Han, Rui Wang 等 2019-07-01 置信度 0.74 Molecular Plant
• Multiplex Genome Engineering Methods for Yeast Cell Factory Development
  As biotechnological applications of synthetic biology tools including multiplex genome engineering are expanding rapidly, the construction of strategically designed yeast cell factories becomes increasingly possible. This is largely due to recent advancements …
  semanticscholar K. Malcı, L. Walls, L. Rios‐Solis 2020-10-29 置信度 0.74 Frontiers in Bioengineering and Biotechnology
• Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii.
  The real value of gas-fermenting clostridia, capable of using CO and CO2, resides in their potential of being developed into cell factories to produce various bulk chemicals and fuels. This process requires rapid chromosomal integration of heterologous chemica…
  semanticscholar He Huang, Changsheng Chai, Sheng Yang, Weihong Jiang 等 2019-03-01 置信度 0.74 Metabolic Engineering
• Sheep and Goat Genome Engineering: From Random Transgenesis to the CRISPR Era
  Sheep and goats are valuable livestock species that have been raised for their production of meat, milk, fiber, and other by-products. Due to their suitable size, short gestation period, and abundant secretion of milk, sheep and goats have become important mod…
  semanticscholar P. Kalds, Shiwei Zhou, Bei Cai, Jiao Liu 等 2019-09-03 置信度 0.74 Frontiers in Genetics
• A Multipurpose Toolkit to Enable Advanced Genome Engineering in Plants[OPEN]
  An integrated reagent toolkit and streamlined protocols work across diverse plant species to enable sophisticated genome edits. We report a comprehensive toolkit that enables targeted, specific modification of monocot and dicot genomes using a variety of genom…
  semanticscholar T. Čermák, Shaun J. Curtin, J. Gil-Humanes, R. Čegan 等 2017-05-18 置信度 0.74 The Plant Cell
• Precision genome engineering through adenine base editing in plants
  semanticscholar Beum-Chang Kang, Jae-Young Yun, Sang-Tae Kim, Youjin Shin 等 2018-06-04 置信度 0.74 Nature Plants
• Biology and Applications of CRISPR Systems: Harnessing Nature's Toolbox for Genome Engineering.
  semanticscholar A. V. Wright, James K. Nuñez, J. Doudna 2016-01-14 置信度 0.74 Cell
• CRISPR–Cas9 System for Genome Engineering of Photosynthetic Microalgae
  semanticscholar V. K. Patel, Niraja Soni, Venkatesh Prasad, A. Sapre 等 2019-05-28 置信度 0.74 Molecular Biotechnology
• Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering
  semanticscholar Chaoyong Huang, Liwei Guo, Jingge Wang, Ning Wang 等 2020-01-28 置信度 0.74 Applied Microbiology and Biotechnology
• CRISPR/Cas-based genome engineering in natural product discovery.
  Covering: up to February, 2018This review briefly introduces and summarizes current knowledge about the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) - CRISPR/Cas system and how it was engineered to become one of th…
  semanticscholar Y. Tong, T. Weber, S. Lee 2019-09-18 置信度 0.74 Natural product reports (Print)
• Genome engineering of Eubacterium limosum using expanded genetic tools and CRISPR-Cas9 system.
  Eubacterium limosum is one of the important bacteria in C1 feedstock utilization as well as in human gut microbiota. Although E. limosum has recently garnered much attention and investigation on a genome-wide scale, a bottleneck for systematic engineering in E…
  semanticscholar Jongoh Shin, Seulgi Kang, Yoseb Song, Sangrak Jin 等 2019-08-02 置信度 0.74 ACS Synthetic Biology
• CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
  In just 3 years CRISPR genome editing has transformed biology, and its popularity and potency continue to grow. New CRISPR effectors and rules for locating optimum targets continue to be reported, highlighting the need for computational CRISPR targeting tools …
  semanticscholar Kornel Labun, Tessa G. Montague, James A. Gagnon, Summer B. Thyme 等 2016-05-16 置信度 0.74 Nucleic Acids Res.
• Retrons and their applications in genome engineering
  Abstract Precision genome editing technologies have transformed modern biology. These technologies have arisen from the redirection of natural biological machinery, such as bacteriophage lambda proteins for recombineering and CRISPR nucleases for eliciting sit…
  semanticscholar Anna J. Simon, A. Ellington, I. Finkelstein 2019-10-10 置信度 0.74 Nucleic Acids Research
• Nanomaterial based gene delivery: a promising method for plant genome engineering.
  Nanomaterials have attracted considerable attention from researchers in recent years due to their unique architecture and small dimensions. Significant progress has been made in the therapeutics, diagnostics, and delivery of biomolecules in animal cells. Howev…
  semanticscholar Sanjeev K. Jat, J. Bhattacharya, Manoj K. Sharma 2020-04-14 置信度 0.74 Journal of materials chemistry. B
• Towards high-throughput genome engineering in lactic acid bacteria.
  Lactic acid bacteria play a vital role as starter cultures in the fermentation of a variety of foods, often altering the flavor and texture in addition to aiding in preservation. Their importance to the industry has made them targets for metabolic engineering …
  semanticscholar S. M. Rothstein, Swastik Sen, Thomas J. Mansell 2020-01-23 置信度 0.74 Current Opinion in Biotechnology
• Current situation of biofuel production and its enhancement by CRISPR/Cas9-mediated genome engineering of microbial cells.
  Geopolitical and economic factors have motivated the scientific community to utilize renewable energy resources. In addition to the modifications in major steps and processes of biofuel production, manipulation of microbial genome engineering tools is essentia…
  semanticscholar M. Javed, M. Noman, M. Shahid, T. Ahmed 等 2019-02-01 置信度 0.74 Microbiology Research
• An integrated platform for genome engineering and gene expression perturbation in Plasmodium falciparum
  Establishing robust genome engineering methods in the malarial parasite, Plasmodium falciparum, has the potential to substantially improve the efficiency with which we gain understanding of this pathogen’s biology to propel treatment and elimination efforts. M…
  semanticscholar Armiyaw S. Nasamu, A. Falla, C. Pasaje, Bridget A. Wall 等 2019-10-24 置信度 0.74 Scientific Reports
• Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized
  In recent years mitochondrial DNA (mtDNA) has transitioned to greater prominence across diverse areas of biology and medicine. The recognition of mitochondria as a major biochemical hub, contributions of mitochondrial dysfunction to various diseases, and sever…
  semanticscholar P. Gammage, C. Moraes, M. Minczuk 2017-11-24 置信度 0.74 Trends in Genetics
• Near-infrared optogenetic genome engineering based on photon upconversion hydrogels.
  Photon upconversion (UC) from near-infrared (NIR) light to visible light has enabled optogenetic manipulations in deep tissues. However, materials for NIR optogenetics have been limited to inorganic UC nanoparticles. Extension to organic triplet-triplet annihi…
  semanticscholar Y. Sasaki, M. Oshikawa, Pankaj Bharmoria, Hironori Kouno 等 2019-10-21 置信度 0.74 Angewandte Chemie
• Using CRISPR/Cas9 for multiplex genome engineering to optimize the ethanol metabolic pathway in Saccharomyces cerevisiae
  Abstract Despite the great progress in genome editing in the model organism Saccharomyces cerevisiae, regulating the carbon flux to ethanol in the ethanol metabolic pathway to achieve high ethanol yield and productivity remains a challenge. Here, we developed …
  semanticscholar Kui Liu, Xue Yuan, Limin Liang, Jingping Fang 等 2019-05-01 置信度 0.74 Biochemical engineering journal
• Applying CRISPR/Cas for genome engineering in plants: the best is yet to come.
  semanticscholar H. Puchta 2017-04-01 置信度 0.74 Current opinion in plant biology
• Inducible Plasmid Self-Destruction (IPSD) assisted genome engineering in lactobacilli and bifidobacteria
  Genome engineering is essential for application of synthetic biology in probiotics including lactobacilli and bifidobacteria. Several homologous recombination system-based mutagenesis tools have been developed for these bacteria but still, have many limitation…
  semanticscholar F. Zuo, Z. Zeng, L. Hammarström, H. Marcotte 2019-05-24 置信度 0.74 bioRxiv
• Germline Cas9 expression yields highly efficient genome engineering in a major worldwide disease vector, Aedes aegypti
  Significance Aedes aegypti is the principal vector of multiple arboviruses that significantly affect human health, including dengue, chikungunya, and zika. Development of tools for efficient genome engineering in this mosquito will not only lay the foundation …
  semanticscholar Ming Li, Michelle Bui, Ting Yang, Christian S. Bowman 等 2017-06-27 置信度 0.74 Proceedings of the National Academy of Sciences of the United States of America
• Repurposing CRISPR-Cas12b for mammalian genome engineering
  The prokaryotic CRISPR-Cas adaptive immune systems provide valuable resources to develop genome editing tools, such as CRISPR-Cas9 and CRISPR-Cas12a/Cpf1. Recently, CRISPR-Cas12b/C2c1, a distinct type V-B system, has been characterized as a dual-RNA-guided DNA…
  semanticscholar Fei Teng, Tongtong Cui, Guihai Feng, Lu Guo 等 2018-10-27 置信度 0.74 Cell Discovery
• Organizing genome engineering for the gigabase scale
  Genome-scale engineering holds great potential to impact science, industry, medicine, and society, and recent improvements in DNA synthesis have enabled the manipulation of megabase genomes. However, coordinating and integrating the workflows and large teams n…
  semanticscholar Bryan A. Bartley, J. Beal, Jonathan R. Karr, E. Strychalski 2019-09-03 置信度 0.74 Nature Communications
• i-GONAD: a robust method for in situ germline genome engineering using CRISPR nucleases
  We present a robust method called improved-Genome editing via Oviductal Nucleic Acids Delivery (i-GONAD) that delivers CRISPR ribonucleoproteins to E0.7 embryos via in situ electroporation. The method generates mouse models containing single-base changes, kilo…
  semanticscholar M. Ohtsuka, Masahiro Sato, Hiromi Miura, Shuji Takabayashi 等 2018-02-26 置信度 0.74 Genome Biology
• Viral Vectors for Plant Genome Engineering
  Recent advances in genome engineering (GE) has made it possible to precisely alter DNA sequences in plant cells, providing specifically engineered plants with traits of interest. Gene targeting efficiency depends on the delivery-method of both sequence-specifi…
  semanticscholar Syed Shan-e-Ali Zaidi, S. Mansoor 2017-04-11 置信度 0.74 Frontiers in Plant Science
• Precise Editing at DNA Replication Forks Enables Multiplex Genome Engineering in Eukaryotes
  SUMMARY We describe a multiplex genome engineering technology in Saccharomyces cerevisiae based on annealing of synthetic oligonucleotides at the lagging strand of DNA replication. The mechanism is independent of Rad51-directed homologous recombination and avo…
  semanticscholar Edward M Barbieri, Paul Muir, Benjamin O. Akhuetie-Oni, C. Yellman 等 2017-11-16 置信度 0.74 Cell
• Modulating DNA Repair Pathways to Improve Precision Genome Engineering.
  semanticscholar K. Pawelczak, Navnath S. Gavande, P. VanderVere-Carozza, J. Turchi 2017-12-20 置信度 0.74 ACS Chemical Biology
• Multiplexed site-specific genome engineering for overproducing bioactive secondary metabolites in actinomycetes.
  semanticscholar Lei Li, Guosong Zheng, Jun Chen, M. Ge 等 2017-03-01 置信度 0.74 Metabolic Engineering
• Genome Engineering and Agriculture: Opportunities and Challenges
  In recent years, plant biotechnology has witnessed unprecedented technological change. Advances in high-throughput sequencing technologies have provided insight into the location and structure of functional elements within plant DNA. At the same time, improvem…
  semanticscholar N. Baltes, J. Gil-Humanes, D. Voytas 2017-02-17 置信度 0.74 Progress in Molecular Biology and Translational Science
• CRISPR EnAbled Trackable genome Engineering for isopropanol production in Escherichia coli.
  semanticscholar Liya Liang, Rongming Liu, Andrew D. Garst, Thomas Lee 等 2017-05-01 置信度 0.74 Metabolic Engineering
• Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable Phenotype
  CRISPR/Cas9 genome engineering strategies allow the directed modification of the Caenorhabditis elegans genome to introduce point mutations, generate knock-out mutants, and insert coding sequences for epitope or fluorescent tags. Three practical aspects, howev…
  semanticscholar Sonia El Mouridi, C. Lecroisey, Philippe Tardy, M. Mercier 等 2017-02-03 置信度 0.74 G3: Genes, Genomes, Genetics
• Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays
  The CRISPR/Cas9 system is an RNA guided nuclease system that evolved as a mechanism of adaptive immunity in bacteria. This system has been adopted for numerous genome engineering applications in research and recently, therapeutics. The CRISPR/Cas9 system has b…
  semanticscholar M. Kurata, Natalie K. Wolf, Walker S. Lahr, Madison T. Weg 等 2018-05-25 置信度 0.74 bioRxiv
• Genome Engineering of Stem Cells for Autonomously Regulated, Closed-Loop Delivery of Biologic Drugs
  Summary Chronic inflammatory diseases such as arthritis are characterized by dysregulated responses to pro-inflammatory cytokines such as interleukin-1 (IL-1) and tumor necrosis factor α (TNF-α). Pharmacologic anti-cytokine therapies are often effective at dim…
  semanticscholar Jonathan M. Brunger, Ananya Zutshi, V. P. Willard, C. Gersbach 等 2017-04-27 置信度 0.74 Stem Cell Reports
• Off-target Effects in CRISPR/Cas9-mediated Genome Engineering
  CRISPR/Cas9 is a versatile genome-editing technology that is widely used for studying the functionality of genetic elements, creating genetically modified organisms as well as preclinical research of genetic disorders. However, the high frequency of off-target…
  semanticscholar Xiao-Hui Zhang, L. Tee, Xiaogang Wang, Qunshan Huang 等 2015-11-01 置信度 0.74 Molecular Therapy: Nucleic Acids
• High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration
  Efficient genome engineering is critical to understand and use microbial functions. Despite recent development of tools such as CRISPR-Cas gene editing, efficient integration of exogenous DNA with well-characterized functions remains limited to model bacteria.…
  semanticscholar Joshua R. Elmore, Gara N. Dexter, H. Baldino, Jay D Huenemann 等 2023-03-01 置信度 0.74 Science Advances
• DNA Break Repair in Plants and Its Application for Genome Engineering.
  semanticscholar C. Schmidt, M. Pacher, H. Puchta 2018-11-11 置信度 0.74 Methods in molecular biology
• Development of genome engineering technologies in cattle: from random to specific
  The production of transgenic farm animals (e.g., cattle) via genome engineering for the gain or loss of gene functions is an important undertaking. In the initial stages of genome engineering, DNA micro-injection into one-cell stage embryos (zygotes) followed …
  semanticscholar S. Yum, K. Youn, W. Choi, G. Jang 2018-01-30 置信度 0.74 Journal of Animal Science and Biotechnology
• Genome engineering for microbial natural product discovery.
  The discovery and development of microbial natural products (MNPs) have played pivotal roles in the fields of human medicine and its related biotechnology sectors over the past several decades. The post-genomic era has witnessed the development of microbial ge…
  semanticscholar Sisun Choi, Yohei Katsuyama, Linquan Bai, Z. Deng 等 2018-10-01 置信度 0.74 Current Opinion in Microbiology
• Efficient engineering of human and mouse primary cells using peptide-assisted genome editing
  Simple, efficient and well-tolerated delivery of CRISPR genome editing systems into primary cells remains a major challenge. Here we describe an engineered Peptide-Assisted Genome Editing (PAGE) CRISPR–Cas system for rapid and robust editing of primary cells w…
  semanticscholar Zhen Zhang, A. Baxter, Diqiu Ren, Kunhua Qin 等 2023-04-24 置信度 0.74 Nature Biotechnology
• Multiplex genome engineering: Methodologies and applications.
  europepmc 2026 置信度 0.80
• Multiplex genome engineering in yeast using the TIGR-Tas system
  europepmc 2026 置信度 0.80
• Diversity and genome engineering applications of R2 non-LTR retrotransposons.
  europepmc 2026 置信度 0.80
• Flying under the radar: immune-evasive DNA for genome engineering.
  europepmc 2026 置信度 0.80
• Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi.
  europepmc 2026 置信度 0.80
• Prime editing for precise genome engineering and modulation of fungal metabolism.
  europepmc 2026 置信度 0.80
• Regulator-Guided Strain Prioritization and Genome Engineering Uncovers Concanamycin Analogs.
  europepmc 2026 置信度 0.80
• Synthetic biology and genome engineering in support of human, agricultural, and environmental security.
  europepmc 2026 置信度 0.80
• Multi-Targeting Non-Specific Genome Engineering in Bacteria.
  europepmc 2026 置信度 0.80
• A versatile "all-in-one" episomal system simplifies genome engineering in diatoms.
  europepmc 2026 置信度 0.80
• Fourth-generation gene editors: Integration-based genome engineering.
  europepmc 2026 置信度 0.80
• Multiplexed, precise genome engineering in monocots with twin prime editing systems.
  europepmc 2026 置信度 0.80
• Sonogenetics for Precision Medicine: A Focus on Immunoengineering and Genome Engineering.
  europepmc 2026 置信度 0.80
• Potential unlocked: an atlas of cloned wheat genes for genome engineering and breeding.
  europepmc 2026 置信度 0.80
• Orthogonal Transposons for Iterative Genome Engineering of Mammalian Cells
  europepmc 2026 置信度 0.80
• CRISPR-Cas9: Genome Engineering and Future Vaccine Applications.
  europepmc 2026 置信度 0.80
• Pythia provides deep learning-driven precision in CRISPR-Cas9 genome engineering.
  europepmc 2026 置信度 0.80
• Discovery of a chimeric transposase-transposon system for advanced genome engineering.
  europepmc 2026 置信度 0.80
• Chloroplast genome engineering of potato enables diterpene production without agronomic penalty
  europepmc 2026 置信度 0.80
• Compound delivery of eVLPs enhances prime editing for targeted genome engineering and high-throughput screening.
  europepmc 2026 置信度 0.80
• An advanced genome engineering platform for Komagataella phaffii enabling versatile in vivo DNA assembly and multiplex integration.
  europepmc 2026 置信度 0.80
• Prime editing: a new frontier in precision genome engineering for rice improvement.
  europepmc 2026 置信度 0.80
• Innovations in Bacteriophage Genome Engineering for Combating Multidrug-Resistant Bacterial Infections.
  europepmc 2026 置信度 0.80
• CRISPR/Cas9 Genome Engineering in Non-Conventional Oleaginous Yeasts: Applications, Challenges, and Prospects.
  europepmc 2026 置信度 0.80
• A compact engineered TnpB editor enables efficient genome engineering in non-model industrial microorganisms
  europepmc 2026 置信度 0.80
• Mammalian genome writing: Unlocking new length scales for genome engineering.
  europepmc 2026 置信度 0.80
• Conjugation-based genome engineering enables rapid prototyping and bioproduction in non-model bacteria
  europepmc 2026 置信度 0.80
• Phage genome engineering with retrons.
  europepmc 2025 置信度 0.80
• Applications of multiplexed CRISPR-Cas for genome engineering.
  europepmc 2025 置信度 0.80
• An Efficient Workflow for CHO Cell Genome Engineering with OpenCRISPR-1
  europepmc 2025 置信度 0.80
• SETDB1/ATF7IP regulate the precise genome engineering of HUSH-regulated genes.
  europepmc 2026 置信度 0.80