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• Synthetic transporters for oxoanions
  crossref Karolis Norvaisa, Aaron Torres-Huerta, Hennie Valkenier 2024-11-13T21:02:45Z 置信度 0.70
• Red light responsive Cre recombinase for bacterial optogenetics
  Abstract Optogenetic tools have been used in a wide range of microbial engineering applications that benefit from the tunable, spatiotemporal control that light affords. However, the majority of current optogenetic constructs for bacteria respond to blue light…
  crossref Fereshteh Jafarbeglou, Mary J. Dunlop 2024-05-31T06:55:15Z 置信度 0.70
• Structure-based approaches in synthetic lethality strategies
  crossref Francesco Rinaldi, Stefania Girotto 2024-08-12T15:30:35Z 置信度 0.70
• Decision: Challenges in observing transcription–translation for bottom-up synthetic biology — R1/PR8
  crossref 2025-01-28T03:01:02Z 置信度 0.70
• Self-organized spatial targeting of contractile actomyosin rings for synthetic cell division
  Abstract One of the challenges of bottom-up synthetic biology is the engineering of a minimal module for self-division of synthetic cells. To produce the contractile forces required for the controlled excision of cell-like compartments such as giant unilamella…
  preprints María Reverte-López, Nishu Kanwa, Yusuf Qutbuddin, Marion Jasnin 等 2024 置信度 0.74
• Successful adaptation of a MinION nanopore for protein sequencing
  crossref Casey-Tyler Berezin 2024-11-07T15:31:45Z 置信度 0.70
• eLife Assessment: De novo-designed minibinders expand the synthetic biology sensing repertoire
  crossref Ahmad S Khalil 2024-10-30T11:10:51Z 置信度 0.70
• Recommendation: Challenges in observing transcription–translation for bottom-up synthetic biology — R1/PR7
  crossref 2025-01-28T03:01:02Z 置信度 0.70
• Recommendation: Challenges in observing transcription–translation for bottom-up synthetic biology — R0/PR4
  crossref 2025-01-28T03:01:02Z 置信度 0.70
• Synthetic Promoters
  crossref 2024-07-27T20:39:54Z 置信度 0.70
• Life from the Edge of Synthetic Biology
  crossref 2024-05-29T13:56:41Z 置信度 0.70
• Decision: Challenges in observing transcription–translation for bottom-up synthetic biology — R0/PR5
  crossref 2025-01-28T03:01:02Z 置信度 0.70
• CHARACTERIZATION OF RECOMBINASE ACTIVITY ACROSS CELLULAR GROWTH PHASES
  ABSTRACT Recombinases, which are enzymes that catalyze targeted DNA modifications, hold significant potential in synthetic biology. Their capacity to precisely manipulate genetic material enables the construction of complex genetic circuits that can be dynamic…
  preprints M. Gonzalez-Colell, J. Macia 2024 置信度 0.74
• eLife Assessment: De novo-designed minibinders expand the synthetic biology sensing repertoire
  crossref Ahmad S Khalil 2024-04-19T08:25:58Z 置信度 0.70
• Detection of MicroRNAs Using Synthetic Toehold Switch in Mammalian Cells
  crossref Yuwen Zhao, Pratima Poudel, Shue Wang 2024-03-05T16:08:56Z 置信度 0.70
• Genetic encoding and expression of RNA origami cytoskeletons in synthetic cells
  Abstract The central dogma at the core of molecular biology states that information flows from DNA to RNA and then to protein. Our research seeks to introduce a conceptually novel approach towards synthetic life by leveraging RNA origami, as an alternative to …
  preprints Mai P. Tran, Taniya Chakraborty, Erik Poppleton, Luca Monari 等 2024 置信度 0.74
• Synthetic Life and Evolutionary Algorithms: Cutting-Edge Innovations in Bioinformatics(Computational Biology)
  Abstract: This chapter, "Synthetic Life and Evolutionary Algorithms: Cutting-Edge Innovations in Bioinformatics," delves into the revolutionary advancements in synthetic biology and evolutionary computation within the field of bioinformatics. It provides a his…
  crossref Murali Krishna Pasupuleti 2024-08-07T11:59:05Z 置信度 0.70
• The future of cell-free synthetic biology
  crossref Yuan Lu 2024-11-09T18:55:55Z 置信度 0.70
• Efficient Simulation of Viral Transduction and Propagation for Biomanufacturing
  Abstract Viral transduction is a main route for gene transfer to producer cells in biomanufacturing. Designing a transduction-based biomanufacturing process poses significant challenges, due to the complex dynamics of viral infection and virus-host interaction…
  preprints Francesco Destro, Richard D. Braatz 2024 置信度 0.74
• Enhancers: A Focus on Synthetic Biology and Correlated Gene Expression
  crossref Christopher H. Bohrer, Nadezda A. Fursova, Daniel R. Larson 2024-09-14T16:58:41Z 置信度 0.70
• Author comment: Challenges in observing transcription–translation for bottom-up synthetic biology — R0/PR1
  crossref Gijs Wuite 2025-01-28T03:01:02Z 置信度 0.70
• MAIA: An open-source, modular, bioreactor for cities
  Abstract This work presents the design and fabrication of MAIA, an open-source, modular, low-cost, and portable bioreactor for democratizing the development of synthetic biology based projects for urban settings. The integration of open-source synthetic biolog…
  crossref Andres Rico, David Kong, Kent Larson 2024-05-05T23:55:16Z 置信度 0.70
• Author comment: Challenges in observing transcription–translation for bottom-up synthetic biology — R1/PR6
  crossref Gijs Wuite 2025-01-28T03:01:02Z 置信度 0.70
• Harnessing Synthetic Biology for Sustainable Industrial Innovation: Advances, Challenges, and Future Direction
  crossref Emmanuel Chimeh Ezeako 2024-12-16T18:14:14Z 置信度 0.70
• Synthetic Promoter Design and Functional Evaluation in Saccharomyces cerevisiae
  crossref Chufan Xiao, Xiufang Liu, Mingtao Huang 2024-07-27T20:39:54Z 置信度 0.70
• Index
  crossref 2024-11-05T06:23:36Z 置信度 0.70
• Frontmatter
  crossref 2024-11-05T06:23:36Z 置信度 0.70
• Regulating ferredoxin electron transfer using nanobody and antigen interactions
  Abstract Fission and fusion can be used to generate new regulatory functions in proteins. This approach has been used to create ferredoxins (Fd) whose cellular electron transfer is dependent upon small molecule binding. To investigate whether Fd fragments can …
  crossref Albert Truong, Jonathan J. Silberg 2024-10-25T01:25:20Z 置信度 0.70
• Self-Documenting Plasmids
  S ummary Plasmids are the workhorse of biotechnology. These small DNA molecules are used to produce recombinant proteins and to engineer living organisms. They can be regarded as the blueprints of many biotechnology products. It is, therefore, critical to ensu…
  crossref Sarah I. Hernandez, Samuel J. Peccoud, Casey-Tyler Berezin, Jean Peccoud 2024-10-30T23:40:16Z 置信度 0.70
• Contents
  crossref 2024-11-05T06:23:36Z 置信度 0.70
• Biological Engineering for Carbon Solutions: Advancing Synthetic Approaches to Carbon Capture
  Abstract: Abstract: This chapter explores the role of biological engineering and synthetic biology in developing advanced carbon capture solutions to address climate change. It highlights how engineered microbes, enzymes, and synthetic metabolic pathways are b…
  crossref Murali Krishna Pasupuleti 2024-10-08T10:16:21Z 置信度 0.70
• Natural Selection in a Synthetic Yeast Endosymbiont Promotes Stable Coexistence
  ABSTRACT Bacteria engulfment by a higher order host is believed to be the beginning of an evolutionary process that ultimately formed mitochondria. In an effort to experimentally elucidate the early effects of natural selection on bacteria resident in a eukary…
  crossref Lubica Supekova, Han Zhou, Izabella H. Barcellos, Catherine Nguyen 等 2024-08-01T02:05:15Z 置信度 0.70
• Regulating biocondensates within synthetic cells via segregative phase separation
  Living cells orchestrate a myriad of biological reactions within a highly complex and crowded environment. A major factor responsible for such seamless assembly are the preferential interactions between the constituent macromolecules, either associative or seg…
  preprints Chang Chen, Caroline M. Love, Christopher F. Carnahan, Ketan A. Ganar 等 2024 置信度 0.74
• Mutual dependence between membrane phase separation and bacterial division protein dynamics in synthetic cell models
  Abstract Cell membranes in bacteria are laterally polarized to produce specific environments for membrane proteins, e.g., proteins involved in cell division which accumulate at mid-cell or the cell poles. An interesting result of such membrane-lipid interplay …
  crossref Nishu Kanwa, Shunshi Kohyama, Leonard Fröhlich, Amogh Desai 等 2024-09-17T23:50:20Z 置信度 0.70
• Therapeutic synthetic anion transporters
  crossref Elba Feo, Philip A. Gale 2024-09-27T19:48:42Z 置信度 0.70
• Hybrid Synthetic Promoters in Saccharomyces cerevisiae Built on Foreign Promoter Sequences
  crossref Xiaofan Feng, Mario Andrea Marchisio 2024-07-27T20:39:54Z 置信度 0.70
• Synthetic Biology: A New Frontier
  crossref Ray J Rose 2024-11-15T11:03:43Z 置信度 0.70
• Engineering inter-kingdom adrenergic signaling in commensals couples host stress hormone sensing to programmable biological outputs.
  europepmc 2026 置信度 0.80
• Biosynthesis of Fluorinated Aromatic Polyketides Through Directed Evolution of Fused Type III Polyketide Synthase.
  europepmc 2026 置信度 0.80
• Microbial C1 assimilation pathways for chemical synthesis: from native metabolism to synthetic design.
  C1 compounds are abundant, non-food and renewable feedstocks, making them attractive substrates for producing value-added chemicals via microbial bioconversion. In nature, autotrophic microorganisms assimilate C1 substrates, including CO, CO 2 , methane, metha…
  pubmed He Y, Foo JL, Choi WJ, Xu P 等 2026 置信度 0.82
• Toward Complex Polyketide Biosynthesis in <i>Yarrowia lipolytica</i> via Metabolic Reprogramming.
  Polyketides are among the most structurally diverse and therapeutically important classes of natural products, serving as antibiotics, anticancer agents, agrochemicals, and industrial pigments. Their structural complexity and limited natural availability have …
  pubmed Ghaempanah K, Son SH, Lee JY 2026 置信度 0.82
• A Modular Liver-Microbial Microfluidic Platform to Evaluate Therapeutic and Adverse Effects of Microbial Metabolites.
  europepmc 2026 置信度 0.80
• Microbial remodeling of bile acids: emerging engineering strategies for precision fermentation and functional food innovation.
  Bile acids (BAs) are emerging as key signaling metabolites at the interface of diet, the gut microbiota, and host physiology. Microbial transformation generates structurally diverse BA species that regulate host metabolism and immunity via receptor-mediated si…
  pubmed Lee GW, Song HY, Kim BM, Hwang IY 等 2026 置信度 0.82
• SARGE: Serine Integrase-Assisted Rapid Genome Integration through Cassette Exchange in <i>Escherichia coli</i>.
  europepmc 2026 置信度 0.80
• MOZAIC: compound growth via in silico reactions and global optimization using Conformational Space Annealing.
  Fragment-based drug discovery (FBDD) efficiently explores chemical space by combining small molecular fragments. Advances in computational methods are accelerating the development of algorithm- and AI-based approaches in FBDD. However, it should be noted that …
  pubmed Yoo J, Shin WH 2026 置信度 0.82
• Engineering red cells across scales: Red Blood Cells (RBCs) and RBC-derived extracellular vesicles.
  europepmc 2026 置信度 0.80
• Engineered commensals for metabolic modulation of the gut-liver-brain axis.
  europepmc 2026 置信度 0.80
• Engineered Probiotics Protect against <i>Pseudomonas aeruginosa</i> Post Antibiotic Exposure.
  europepmc 2026 置信度 0.80
• The MDPV Derivative α-PHP Regulates Cellular Differentiation and Triggers Apoptotic Cell Death and Ultrastructural Changes in Murine 3D Neurospheres.
  europepmc 2026 置信度 0.80
• GSDME mediated pyroptosis drives CCL5 secretion and dictates proteasome inhibitor efficacy in solid tumors.
  Proteasome inhibitors show limited efficacy against solid tumors. We previously show the combination of Bortezomib with ammonium tetrathiomolybdate (TM) or AMD3100 could inhibit breast cancer growth rely on intact immune system. However, it remains to be explo…
  pubmed Tang D, Lei JH, Lin S, Zhou J 等 2026 置信度 0.82
• IS110 transposon utilizes two mechanistically distinct RNA-guided transposition pathways.
  europepmc 2026 置信度 0.80
• AI and mechanistic modeling for characterizing biosynthetic pathways of natural products.
  Covering: 2020 to 2025Natural products are a major source of bioactive compounds, yet elucidating their biosynthetic pathways remains a major challenge due to complex genotype-phenotype relationships. Recent advances in computational approaches, particularly a…
  europepmc 2026 置信度 0.80
• Evaluation of S7, a synthetic partial abscisic acid agonist, as a potential suppressor of pre-harvest sprouting in rice.
  Pre-harvest sprouting (PHS) is a major constraint affecting grain quality and yield in rice, and its incidence is expected to increase with more frequent rainfall events associated with climate change. Although chemical control of PHS has largely focused on ab…
  pubmed Kim JH, Mun JH, Jo SH, Baek JK 等 2026 置信度 0.82
• Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.
  europepmc 2026 置信度 0.80
• The Mevalonate Pathway: Innovations, Applications, and Challenges in Biotechnology with Emphasis on Fungal Biology.
  The mevalonate (MVA) pathway is a central metabolic route responsible for the biosynthesis of isoprenoids with broad biological and biotechnological relevance. Due to its importance, the MVA pathway has attracted increasing interest in studies of enzymatic reg…
  pubmed Valle Garay A, Marques Coelho C, Fonseca Valadares N, Ferreira da Silva L 等 2026 置信度 0.82
• NucleiSky enables cross-scale multimodal registration of microscopy data using nuclei constellations.
  Integrating tissue-level organisation with sub-cellular resolution and molecular information often requires combining multiple microscopy modalities and scales. However, aligning images acquired with different modalities, settings, or instruments remains chall…
  pubmed Hidalgo-Cenalmor I, Olguín-Olguín A, Prieto C, Ahnlide JK 等 2026 置信度 0.82
• A Highly Stringent Split Intein-Mediated DHFR Selectable Marker Enables Efficient Development of High-Producing CHO Cells for Therapeutic Proteins.
  europepmc 2026 置信度 0.80
• Emerging tools to investigate the contribution of selenium to ferroptosis and beyond.
  Selenium has a paradoxical role in biology, being essential at trace levels yet toxic at slightly higher doses. To operate within this narrow range, organisms have developed specialized pathways for selenium uptake, transport and usage/storage, primarily throu…
  pubmed Fazeli G, Ahn I, Pluth MD, de Paiva REF 等 2026 置信度 0.82
• Microbial chassis selection for niche-specific engineered live biotherapeutics.
  europepmc 2026 置信度 0.80
• Novel Analytical Workflow for Tracing Synthetic Caffeine Adulteration in Amazonian Guaraná (Paullinia cupana) Using Low-Field 1H NMR Fingerprinting and Chemometrics.
  Maués guaraná (Paullinia cupana), a Brazilian product with Geographical Indication (GI), is highly susceptible to economically motivated fraud, particularly through the undeclared addition of synthetic caffeine. Conventional chromatographic methods f…
  pubmed de Andrade JC, de Oliveira PLC, Machado M, de Oliveira AT 等 2026 置信度 0.82
• Bacterial conjugation and its deployment for diverse biotechnology applications.
  Conjugation has emerged as an attractive tool in synthetic biology by delivering designed genetic circuits to diverse microbes simultaneously without isolation. Insights into conjugative machinery have enabled the repurposing of these systems to deliver geneti…
  europepmc 2026 置信度 0.80
• Cell-free synthesis and characterization of Salmonella, Escherichia coli, and Shigella-specific bacteriophages.
  Cell-free gene expression (CFE) systems provide a rapid and modular platform for synthesizing bacteriophages without the need for living host cells. However, the generalizability of CFE-based phage synthesis across diverse phages, as well as the functional com…
  pubmed Iskender I, Bosma J, Soudier P, Guo Y 等 2026 置信度 0.82
• Plant microbiome engineering: from inoculation to genome editing.
  Plant-associated microbiomes are central to crop productivity, nutrient efficiency, and stress resilience, yet conventional microbiome manipulation strategies, largely based on microbial inoculation and agronomic management, often suffer from inconsistent fiel…
  europepmc 2026 置信度 0.80
• Airborne Immunity: A Bacterial Volatile Primes Plant Defenses by Unlocking a Phytoalexin Biosynthetic Checkpoint.
  Microbial volatile organic compounds (mVOCs) enable plants to perceive microbial activity prior to physical contact, yet the contribution of individual bacterial volatiles to immune signalling and disease resistance remains incompletely understood. Here, heads…
  pubmed Paponov IA, Kanchiswamy CN, Bossi S, Caldo F 等 2026 置信度 0.82
• A reversible memory switch for plant synthetic biology based on the phage PhiC31 integration system
  preprints 2019 置信度 0.74
• A synthetic biology platform for the reconstitution and mechanistic dissection of LINC complex assembly
  ABSTRACT The linker of nucleoskeleton and cytoskeleton (LINC) is a conserved nuclear envelope-spanning molecular bridge that is responsible for the mechanical integration of the nucleus with the cytoskeleton. LINC complexes are formed by a transluminal interac…
  preprints Sagardip Majumder, Patrick T. Willey, Maxwell S. DeNies, Allen P. Liu 等 2018 置信度 0.74
• Comparative genomics uncovers genetic diversity and synthetic biology of secondary metabolite production of the genus Trametes
  preprints 2019 置信度 0.74
• Optimization of Yarrowia lipolytica-based consolidated biocatalyst through synthetic biology approach: transcription units and signal peptides shuffling
  preprints 2020 置信度 0.74
• Capturing Multicellular System Designs Using the Synthetic Biology Open Language (SBOL)
  preprints 2018 置信度 0.74
• A synthetic biology approach to probing nucleosome symmetry
  preprints 2017 置信度 0.74
• <i>Kluyveromyces marxianus</i> as a robust synthetic biology platform host
  preprints 2018 置信度 0.74
• The Smell of Synthetic Biology: Engineering Strategies for Aroma Compound Production in Yeast
  preprints 2018 置信度 0.74
• Mobilising Ion Mobility Mass Spectrometry in a Synthetic Biology Analytics Workflow
  preprints 2018 置信度 0.74
• The Software Crisis of Synthetic Biology
  Abstract In fifteen years, Synthetic Biology (SB) has moved from proof-of-concept designs to several flagship achievements. Standardisation efforts are still under way, basic engineering concepts such as modularity and orthogonality are still controversial in …
  preprints Sergi Valverde, Manuel Porcar, Juli Peretó, Ricard V. Solé 2016 置信度 0.74
• Off-target glycans encountered along the synthetic biology route towards humanized N-glycans in <i>Pichia pastoris</i>
  preprints 2020 置信度 0.74
• PartsGenie: an integrated tool for optimising and sharing synthetic biology parts
  preprints 2017 置信度 0.74
• A low cost, open source Turbidostat design for <i>in-vivo</i> control experiments in Synthetic Biology
  Abstract To characterise the dynamics of new engineered systems in Synthetic biology, continuous culture platforms are required. In this paper, after a brief review of the existing machines present in literature, we describe the design and the implementation o…
  preprints Agostino Guarino, Barbara Shannon, Lucia Marucci, Claire Grierson 等 2019 置信度 0.74
• Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory
  Abstract Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway per…
  preprints Christopher M. Jakobson, Danielle Tullman-Ercek, Niall M. Mangan 2017 置信度 0.74
• A sustainable synthetic biology approach for the control of the invasive golden mussel ( <i>Limnoperna fortunei</i> )
  preprints 2018 置信度 0.74
• Characterization of <i>Azotobacter vinelandii</i> and Kits for Its Synthetic Biology Applications
  preprints 2017 置信度 0.74
• Fast and flexible simulation and parameter estimation for synthetic biology using bioscrape
  Abstract In systems and synthetic biology, it is common to build chemical reaction network (CRN) models of biochemical circuits and networks. Although automation and other high-throughput techniques have led to an abundance of data enabling data-driven quantit…
  preprints Anandh Swaminathan, William Poole, Ayush Pandey, Victoria Hsiao 等 2017 置信度 0.74
• Plasmid biology is compressed in host chromosomal architecture
  europepmc 2026 置信度 0.80
• BacilloFlex: A modular DNA assembly toolkit for <i>Bacillus subtilis</i> synthetic biology
  Abstract Bacillus subtilis is a valuable industrial production platform for proteins, a bacterial model for cellular differentiation and its endospores have been proposed as a vehicle for vaccine delivery. As such B. subtilis is a major synthetic biology chass…
  preprints Niels Wicke, David Radford, Valeria Verrone, Anil Wipat 等 2017 置信度 0.74
• Lanthanide protein biosensors with a single ion-binding site
  Rising demand for rare earth elements, including lanthanides (Lns), has intensified environmental pressures and supply-chain vulnerabilities, motivating the development of bio-based methods for their extraction and separation. However, the lack of high-through…
  europepmc 2026 置信度 0.80
• Genome to Phenotype Precision Breeding in Wheat through a Quantitative Systematic Review of 184 Studies on Molecular Technologies for Climate Resilience
  europepmc 2026 置信度 0.80
• Accessing Nature’s diversity through metabolic engineering and synthetic biology
  preprints 2016 置信度 0.74
• From Jurassic Germs to Gymnosperms: A Speculative Strategy for Reconstructing Dinosaur Genomes
  europepmc 2026 置信度 0.80
• A Synthetic Minimal Genome Design Framework for Bacteriophages: Application to <em>Streptococcus mutans</em> and Broad-Host-Range Expression in <em>Escherichia coli</em>
  Minimal genome design, reducing a system to its smallest functional part set, is a core synthetic-biology strategy. Bacteriophages are attractive but underused chassis, yet wild-type phages are difficult to engineer. We present a comparative genomics-guided fr…
  europepmc 2026 置信度 0.80
• Circulating Microbial DNA as a Potential Cancer Biomarker: Technical Challenges and Controlled Evaluation
  preprints 2026 置信度 0.74
• Towards interoperable modeling of toehold-mediated strand exchange circuits across DNA nanotechnology and engineering biology
  Originally developed for DNA nanotechnology, toehold-mediated strand exchange (TMSE) circuits are gaining traction in synthetic biology due to their high programmability, seamless integration with biological components, and robust operation across diverse envi…
  europepmc 2026 置信度 0.80
• Immobilised enzyme reactors for post-production glycan modification of purified glycoproteins
  preprints 2026 置信度 0.74
• Linker-Length Landscape Mapping Enables Coupling of Diverse Synthetic Chemically Induced Dimerization Systems to Molecular Readouts
  preprints 2026 置信度 0.74
• Division of Synthetic Cells Using a Genomically Encoded One-Protein Divisome
  Abstract Synthetic biology is emerging as a powerful tool to understand life by building minimal synthetic cells whose functions are encoded within their genome. An essential function of cells is abscission, the final step of cell division that breaks the last…
  preprints Charu Sharma, Nikki Nafar, Jacob Kerssemakers, José Ángel Fernández 等 2026 置信度 0.74
• <i>Marchantia polymorpha</i> as a simple platform for plant-based production of functional nanobodies
  preprints 2026 置信度 0.74
• Intuitive biology, moral reasoning, and engineering life: essentialist thinking and moral purity concerns shape risk assessments of synthetic biology technologies
  preprints 2018 置信度 0.74
• The Plastisphere in Saline Ecosystems: Quorum Sensing, Halotolerant Fungi and Microplastic Degradation Pathways
  europepmc 2026 置信度 0.80
• Multiplex genome editing by natural transformation (MuGENT) for synthetic biology in <i>Vibrio natriegens</i>
  preprints 2017 置信度 0.74
• Ingressing Minds: Causal Patterns Beyond Genetics and Environment in Natural, Synthetic, and Hybrid Embodiments
  preprints 2026 置信度 0.74
• Synthetic transcriptional control in the malaria parasite <i>Plasmodium falciparum</i>
  preprints 2026 置信度 0.74
• Engineering Binding Efficiency and Interaction Stability of a Thermostable Cohesin–Dockerin Pair on the Bacterial Cell Surface
  preprints 2026 置信度 0.74