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• An updated inventory of genes essential for oxidative phosphorylation identifies a mitochondrial origin in familial Ménière’s disease
  Summary Mitochondrial disorders (MDs) are among the most common inborn errors of metabolism and primarily arise from defects in oxidative phosphorylation (OXPHOS). Their complex mode of inheritance and diverse clinical presentations render the diagnosis of MDs…
  preprints 2025 置信度 0.81
• Early mitochondrial gene dysregulation precedes motor neuron degeneration in genomically humanised FUS mutant mice
  Abstract Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder, with mutations in the Fused in Sarcoma ( FUS ) gene accounting for some of the most aggressive forms of the disease. Although FUS mutations are rare, accounting for only around…
  preprints 2026 置信度 0.81
• Repressive Roles of Erf and Elk in FGF-Regulated Neural Development in Ciona intestinalis
  Abstract Insights from urochordates/tunicates can instruct on the evolutionary origins of key cell types or gene regulatory mechanisms such as for the ‘new head’ sensory placodes and neural crest. Taking advantage of their invariant lineage with reproducible b…
  preprints 2025 置信度 0.81
• CRISPR-CLEAR: Nucleotide-Resolution Mapping of Regulatory Elements via Allelic Readout of Tiled Base Editing
  ABSTRACT CRISPR tiling screens have advanced the identification and characterization of regulatory sequences but are limited by low resolution arising from the indirect readout of editing via guide RNA sequencing. This study introduces CRISPR-CLEAR , an end-to…
  preprints 2024 置信度 0.81
• Megabase-scale loss of heterozygosity provoked by CRISPR-Cas9 DNA double-strand breaks
  Harnessing DNA double-strand breaks (DSBs) is a powerful approach for gene editing, but it may provoke loss of heterozygosity (LOH), which predisposes to tumorigenesis. To interrogate this risk, we developed a two- color flow cytometry-based system (Flo-LOH), …
  preprints 2024 置信度 0.81
• The role of the nuclear pore complex in the stability of disease-related short tandem DNA repeats
  Nuclear pore complexes (NPCs) mediate selective transport between the nucleus and the cytoplasm, but also contribute to maintaining genome stability. Mutations in NPC genes cause genome instability and sensitivity to DNA damaging agents, and DNA that is diffic…
  preprints 2026 置信度 0.81
• Modulating immune cell fate and inflammation through CRISPR-mediated DNA methylation editing
  ABSTRACT DNA methylation is traditionally associated with gene silencing, but its causal relationship and role in shaping cell fate decisions still need to be fully elucidated. Here, we conducted a genome-wide analysis to investigate the relationship between D…
  preprints 2024 置信度 0.81
• Visual screening of CRISPR/Cas9 editing efficiency based on micropattern arrays for editing porcine cells
  CRISPR/Cas9 technology in conjunction with somatic cell nuclear transplantation (SCNT) provides the primary approach to producing gene-edited pigs, and targeting nuclear donors with CRISPR/Cas9 is crucial. Gene-edited nuclear donors are inefficient due to poor…
  preprints 2024 置信度 0.81
• Knockout of <i>fatty acid elongase1</i> homeoalleles in amphidiploid <i>Brassica juncea</i> leads to undetectable erucic acid in seed oil
  Summary Indian mustard ( Brassica juncea L.) is a major oilseed crop with significant economic and nutritional importance within the Indian subcontinent. While its seed oil offers valuable dietary benefits, including a balanced ratio of human essential fatty a…
  preprints 2024 置信度 0.81
• In vivo CRISPR screens identify key modifiers of CAR T cell function in myeloma
  Chimeric antigen receptor (CAR) T cells are highly effective in hematologic malignancies. However, loss of CAR T cells can contribute to relapse in a significant number of patients. These limitations could potentially be overcome by targeted gene editing to in…
  preprints 2024 置信度 0.81
• Pythia: Non-random DNA repair allows predictable CRISPR/Cas9 integration and gene editing
  Summary CRISPR-based genome engineering holds enormous promise for basic science and therapeutic applications. Integrating and editing DNA sequences is still challenging in many cellular contexts, largely due to insufficient control of the repair process. We f…
  preprints 2024 置信度 0.81
• A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids
  Kinetoplastid parasites cause diseases that threaten human and animal health. To survive transitions between vertebrate hosts and insect vectors, these parasites rely on precise regulation of gene expression to adapt to environmental changes. Since gene regula…
  preprints 2024 置信度 0.81
• Successful Gene Editing of Apolipoprotein E4 to E3 in Brain of Alzheimer Model Mice After a Single IV Dose of Synthetic Exosome-Delivered CRISPR
  Background The gene for apolipoprotein E4 (ApoE4 E4) confers an increased risk for development and lowers the age of onset of Alzheimer’s disease (AD), and is a highly suitable target for CRISPR-based editing because ApoE4 differs from ApoE3 by a single nucleo…
  preprints 2024 置信度 0.81
• Human-specific tandem repeat in <i>CACNA1C</i> modulates responses to neuronal stimulation
  The recent development of long-read sequencing has made it possible to catalog variable number tandem repeats (VNTRs) in the human genome. However, little is known about their functional consequences. Here, we characterized the effect of TRACT, a VNTR that is …
  preprints 2025 置信度 0.81
• Functional rescue of F508del-CFTR through revertant mutations introduced by CRISPR base editing
  Summary Cystic Fibrosis (CF) is a life-shortening autosomal recessive disease caused by mutations in the CFTR gene, resulting in functional impairment of the encoded ion channel. F508del mutation, a trinucleotide deletion, is the most frequent cause of CF affe…
  preprints 2024 置信度 0.81
• CRISPR-GPT for Agentic Automation of Gene Editing Experiments
  Performing effective gene-editing experiments requires a deep understanding of both the CRISPR technology and the biological system involved. Meanwhile, despite their versatility and promise, Large Language Models (LLMs) often lack domain-specific knowledge an…
  preprints 2024 置信度 0.86 computer sciencecs.aics.lgcs.clhuman-computer interaction
• Future landscape of CRISPR and AI in plant gene editing
  crossref Oseias R. Feitosa-Junior 2026-08-28T13:27:27Z 置信度 0.66
• Supplementary file 2. crRNA targeting sequences used for CRISPR/Cas9 gene editing.
  crossref 2019-07-08T16:00:39Z 置信度 0.64
• Gene Editing in Plants
  crossref 2024-03-18T08:02:35Z 置信度 0.66
• Gene Hackers
  crossref 2018-04-19T02:01:55Z 置信度 0.60
• CRISPR gene editing may be able to disable and cure HIV
  crossref Clare Wilson 2024-03-29T20:09:48Z 置信度 0.66
• CRISPR-Based Gene Editing Techniques in Pediatric Neurological Disorders
  The emergence of gene editing technologies offers a unique opportunity to develop mutation-specific treatments for pediatric neurological disorders. Gene editing systems can potentially alter disease trajectory by correcting dysfunctional mutations or therapeu…
  crossref Stephen Chrzanowski, Ranjan Batra 2024-02-01T21:57:21Z 置信度 0.76
• Detailed Insight into Various Classes of the CRISPR/Cas System to Develop Future Crops
  crossref Neha Thakur, Hiralben Lakhani, Siddharth Tiwari 2024-03-18T08:02:35Z 置信度 0.66
• An Analysis of Global Policies and Regulation on Genome Editing in Plants
  crossref Tejaswini Kumawat, Aditi Agarwal, Sonika Saxena, Sudipti Arora 2024-03-18T08:02:35Z 置信度 0.66
• Gene editing with CRISPR in root and tuber crops
  crossref N. Türkölmez, G.Ç. Kılıç, N. Arslan, Y. Özden-Çiftçi 2024-03-22T09:24:05Z 置信度 0.66
• The Gene Trade
  crossref 2016-10-25T07:35:06Z 置信度 0.60
• CRISPR and Gene Editing: A New Frontier in Healthcare
  The potential for significant advancements in disease management is highlighted by this review, which looks at the application of CRISPR gene editing for treating allergies, particularly those related to allergens. It also evaluates the benefits and limitation…
  crossref Digvijay Singh, Mohammed Ismail Iqbal 2025-08-12T10:18:07Z 置信度 0.82 medicinecancerhealth carebiologybiotechnology
• Advancing gene editing by engineering miniature CRISPR-Cas Un1Cas12f1
  crossref Weimin Tang 2024-08-29T06:31:02Z 置信度 0.66
• The Potential of Gene Editing Technology in Agriculture: Cre-Loxp and CRISPR-Cas9
  In recent years, gene editing technology has made breakthrough progress. This article uses Cre-loxP and CRISPR-Cas9 as examples to explore its application prospects in the agricultural field. Current research has made substantial progress in using these techno…
  crossref Rou Duan 2024-06-06T15:51:03Z 置信度 0.82 data sciencecomputer sciencefieldengineeringemerging technologies
• Deciphering the Role of CRISPR/Cas9 in the Amelioration of Abiotic and Biotic Stress Conditions
  crossref Surender Singh, Roni Chaudhary, Siddhant Chaturvedi, Siddharth Tiwari 2024-03-18T08:02:35Z 置信度 0.63
• Comparison of Gene-Editing Approaches for Severe Congenital Neutropenia-Causing Mutations in the <i>ELANE</i> Gene
  Safety considerations for gene therapies of inherited preleukemia syndromes, including severe congenital neutropenia (CN), are paramount. We compared several strategies for CRISPR/Cas9 gene editing of autosomal-dominant ELANE mutations in CD34 + cells from two…
  crossref Malte Ulrich Ritter, Masoud Nasri, Benjamin Dannenmann, Perihan Mir 等 2024-10-22T10:00:21Z 置信度 0.82 medicinebiologypharmacologycomputational biologygenetics
• Approval of the First CRISPR-Cas9 Gene Editing Therapy for Sickle Cell Disease
  On December 8, 2023 the United States Food and Drug Administration (FDA) made a historic decision to approve the first drug harnessing clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 gene editing technology, CASGEVY™ (exagamglogene auto…
  crossref Sean T Campbell 2024-10-03T13:24:12Z 置信度 0.76
• Unlocking the future of medicine: CRISPR-Cas9 gene editing holds the key to transformation
  The Biochemical Society identifies and celebrates outstanding science communication talent in molecular biosciences with its annual Science Communication Prize. Cansu Kizilkaya, from Turkey, won the second prize in the written category for students studying fo…
  crossref Cansu Kizilkaya 2024-02-02T11:52:21Z 置信度 0.82 cell biologywearable computergeneticspopulationinternal medicine
• Next-generation CRISPR-based gene-editing therapies tested in clinical trials
  crossref Natalie Healey 2024-08-05T15:03:41Z 置信度 0.66
• Perspectives and Overview of CRISPR/Cas Technology in Plant Pathogenesis
  crossref Sakshi Patel, Ameeta Sharma, Neha Batra, Ronak Chetani 等 2024-03-18T08:02:35Z 置信度 0.66
• Review for "Application of CRISPR/Cas9 gene editing technique in the study of cancer treatment"
  crossref 2019-06-25T13:25:55Z 置信度 0.64
• Applications of CRISPR/Cas9 gene-editing technology in cancer
  Cancer has been a problematic disorder for many years, many people will develop cancer sometime in their life but there are few ways to cure it. Treatment for cancer in the modern era is a painful and expensive process with no guarantee that it will not come b…
  crossref Chihan Wang 2024-01-08T23:55:13Z 置信度 0.76
• Use of CRISPR/Cas9 Gene Editing Methods to Investigate the Mechanism of Trem2-Dependent Gene Expression in Macrophages
  Triggering Receptor Expressed on Myeloid Cells 2 (TREM2) is a surface receptor expressed in macrophages during tissue injury. This receptor plays a role in driving phagocytosis and dampening inflammation. Because of this, it plays a large part in diseases such…
  crossref Mohnish Alishala 2024-03-03T22:36:47Z 置信度 0.82 biologymedicinebiotechnologycell biologypharmacology
• The CRISPR-Cas System and Clinical Applications of CRISPR-Based Gene Editing in Hematology with a Focus on Inherited Germline Predisposition to Hematologic Malignancies
  Clustered regularly interspaced short palindromic repeats (CRISPR)-based gene editing has begun to transform the treatment landscape of genetic diseases. The history of the discovery of CRISPR/CRISPR-associated (Cas) proteins/single-guide RNA (sgRNA)-based gen…
  crossref Rina Kansal 2024-07-03T06:04:18Z 置信度 0.82 medicinebiologypharmacologyintensive care medicineinternal medicine
• CRISPR innovations in tissue engineering and gene editing
  The CRISPR/Cas9 system is a powerful tool for genome editing, utilizing the Cas9 nuclease and programmable single guide RNA (sgRNA). However, the Cas9 nuclease activity can be disabled by mutation, resulting in catalytically deactivated Cas9 (dCas9). By combin…
  crossref ZahraSadat Razavi, Madjid Soltani, Mohammad Souri, Andre J. van Wijnen 2024-10-18T05:11:10Z 置信度 0.76
• A New Era of CRISPR Technology to Improve Climate Resilience in Rice
  crossref Siddhi Parab, Shalini Tiwari, Sivasubramanian Rajarammohan, Sneh Lata Singla-Pareek 等 2024-03-18T08:02:35Z 置信度 0.63
• Therapeutic applications of CRISPR-Cas9 gene editing
  CRISPR-Cas9 is a gene editing tool used extensively in biological research that is now making its way into clinical therapies. With the first CRISPR therapy obtaining approval by the United States’ Food and Drug Administration (FDA) in late 2023, we look at cl…
  europepmc Aditya Bharti, Joann Mudge 2025 置信度 0.94 medicinecancerpharmacologybiotechnologymedline
• Gene Editing Technologies: CRISPR/Cas9 and Beyond for Genetic Disease Therapy and Research
  Gene editing technologies have revolutionized the field of genetic disease therapy and research. Among these, CRISPR/Cas9 stands out as a versatile tool for precisely targeting and modifying specific sequences within the genome. This paper provides an overview…
  crossref Sanjay Singh 2024-06-30T17:45:05Z 置信度 0.82 data sciencesynthetic biologycomputational biologybiologyengineering
• Nutrient Biofortification in Crop Plants by the CRISPR/Cas9 Technology: A Potential Approach for Sustainable Food Security
  crossref Indu Singh Sankhla, Anil Kumar, Chandra Pal Singh 2024-03-18T08:02:35Z 置信度 0.63
• CRISPR–Cas9 Gene Editing: Curing Genetic Diseases by Inherited Epigenetic Modifications
  Introduction CRISPR-Cas9 gene editing, leveraging bacterial defense mechanisms, offers precise DNA modifications, holding promise in curing genetic diseases. This review critically assesses its potential, analyzing evidence on therapeutic applications, challen…
  crossref Nikhil Deep Kolanu 2024-03-29T19:36:48Z 置信度 0.76
• Gene Editing by Ferrying of CRISPR/Cas Ribonucleoprotein Complexes in Enveloped Virus-Derived Particles
  The invention of next-generation CRISPR/Cas gene editing tools, like base and prime editing, for correction of gene variants causing disease, has created hope for in vivo use in patients leading to wider clinical translation. To realize this potential, deliver…
  crossref Jacob Hørlück Janns, Jacob Giehm Mikkelsen 2024-08-16T07:26:51Z 置信度 0.82 biologymedicinecomputational biologysynthetic biologycancer
• A Review of Gene Editing Technologies in Poultry Breeding: Focus on CRISPR/Cas9
  crossref Yanlin Wang, Qiqi Zhou, Jia Chen 2025-07-11T08:53:36Z 置信度 0.66
• The application of CRISPR/Cas9 technology in plant gene editing
  Due to its remarkable effectiveness, Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)/Cas9 is also highlighted in gene editing with the widespread use of the technique. In order to explore a gene's low temperature tolerance or other consequenc…
  crossref Qiming Cheng 2024-01-08T23:53:06Z 置信度 0.76
• Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing
  crossref 2020-04-07T08:48:17Z 置信度 0.64
• Application of CRISPR/Cas9 gene-editing technology in Alzheimer’s disease
  Alzheimer's disease (AD) is a prevalent neurodegenerative disorder. It triggers metabolic disorders within the body due to genetic mutations, thus leading to irreversible damage to one's memory and brain. The primary pathogenic features of AD are intracellular…
  crossref Xiangjun Zhang 2024-10-14T03:06:28Z 置信度 0.82 medicinediseaseneuroscienceinternal medicinebiology
• Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing
  crossref 2020-04-07T08:48:17Z 置信度 0.64
• Direct Cytosolic Delivery of CRISPR/Cas9-Ribonucleoprotein for Efficient Gene Editing
  crossref 2020-04-07T08:48:17Z 置信度 0.64
• Engineering CjCas9 for Efficient Base Editing and Prime Editing
  The CRISPR-Cas9 system has been applied for clinical applications of gene therapy. Most CRISPR-based gene therapies are derived from Streptococcus pyogenes Cas9, which is challenging to package into a single adeno-associated virus vector and limits its clinica…
  crossref Siyuan Liu, Yingdi Zhao, Qiqin Mo, Yadong Sun 等 2024-11-18T11:13:28Z 置信度 0.82 materials sciencenanotechnologychemical engineeringchemistrycomputational biology
• Review for "Gene Therapy and Gene Editing in Type 1 Diabetes: CRISPR-Based β-Cell Replacement and Treg Immune Modulation Approaches"
  crossref 2026-04-23T21:11:07Z 置信度 0.66
• CRISPR-editing of the virus vector Aedes albopictus cell line C6/36, illustrated by prohibitin 2 gene knockout
  Aedes mosquitoes are important virus vectors. We provide a toolkit for CRISPR-Cas9-editing of difficult-to-knockdown gene previously shown to be refractory to siRNA silencing in mosquito cells, which is pivotal in understanding vector biology, vector competenc…
  crossref Shiu-Wan Chan 2024-06-21T13:00:43Z 置信度 0.76
• A Protocol for CRISPR-Cas9-Mediated Knockout Construction in Actinobacteria
  The adaptation of various CRISPR-associated proteins (Cas) forms different clustered regularly interspaced short palindromic repeat (CRISPR) systems that are revolutionizing biomedical and basic science. CRISPR-Cas9 technology assists the user to engineer the …
  crossref Khushal Khambhati, Gargi Bhattacharjee, Vijai Singh 2024-11-19T12:10:48Z 置信度 0.76
• Gene Editing, Indiscriminate Degradation And Recyclic Catalytic Events In Crispr Cas Technology
  In "genome editing," a type of genetic engineering, DNA is added, taken away, or changed in an organism's genome. Biomedicine, biotechnology, and synthetic biology, just to name a few, have all gained a lot from how often this method is used. Before the editin…
  crossref Dr. Taha Nazir, Hameed A. Mirza, Sahar Radwan, Nida Taha 2024-01-30T03:09:42Z 置信度 0.82 biologycomputational biologycomputer sciencesynthetic biologyneuroscience
• Advancement in Gene Editing for Crop Improvement Highlighting the Application of Pangenomes
  This chapter presents a thorough exploration of the utilization of pangenomes, a sophisticated genetic repository that encompasses the complete genomic diversities among and within diverse species. Through the integration of genomic and phenotypic data, it is …
  crossref Biswajit Pramanik, Sandip Debnath, Anamika Das 2024-03-05T19:34:56Z 置信度 0.76
• Advances in CRISPR/Cas9-Based Gene Editing Technology in Mosquitoes
  As global warming increases, mosquito activity areas are expanding, alongside changes in the natural environment and the misuse of insecticides, thus further increasing mosquito resistance and exposing the world to an elevated risk of mosquito-borne diseases. …
  crossref Xiaohui Liu, Xinyu Zhou, Xiaoxue Xie, Chunxiao Li 2024-09-20T23:10:17Z 置信度 0.82 biologysynthetic biologybiological sciencesmedicineneuroscience
• The Application of CRISPR Gene Editing Technology in PET Biodegradation
  Plastics used in people's daily life bring convenience on the one hand, and on the other hand, a large amount of plastic waste also brings great pollution to the environment. Polyethylene terephthalate (PET) is a polymer polymerization material, which has beco…
  crossref Jingtong Liu, Ke Wu 2024-06-18T18:02:36Z 置信度 0.82 materials sciencechemistrychemical engineeringcatalysisphysics.app-ph
• Advancements in Gene Editing Technologies: From CRISPR to Beyond
  Gene editing technologies have revolutionized the field of genetics, offering unprecedented precision in modifying DNA sequences. This article explores the evolution of gene editing technologies, focusing on CRISPR-Cas9, the most transformative advancement in …
  crossref Dr. Fatima Tariq 2026-04-17T08:10:41Z 置信度 0.82 data sciencetransformative learningknowledge managementengineering ethicsfield
• Machine learning-inferred and energy landscape-guided analyses reveal kinetic determinants of CRISPR/Cas9 gene editing
  Abstract The CRISPR/Cas nucleases system is widely considered the most important tool in genome engineering. However, current methods for predicting on/off-target effects and designing guide RNA (gRNA) rely on purely data-driven approaches or focus solely on t…
  crossref Lei Jin, Risi Liyanage, Dongsheng Duan, Shi-Jie Chen 2024-05-02T13:35:21Z 置信度 0.82 drug discoveryadsorptionstatistical physicsecosystemq-bio.qm
• CRISPR RNA-Guided Gene Editing and its Clinical Research Applications in Hematology with Focus on Inherited Germline Predisposition to Hematologic Malignancies
  Clustered regularly interspaced short palindromic repeats (CRISPR) based gene-editing has begun to transform the treatment landscape of genetic diseases. The history of the discovery of CRISPR/CRISPR-associated (Cas) proteins/single guide RNA (sgRNA)-based gen…
  preprints Rina Kansal 2024 置信度 0.86 medicinepharmacologyintensive care medicineinternal medicinedisease
• Gene Editing Startups Fabricate Industrial-Grade CRISPR Tools
  crossref Gail Dutton 2019-05-29T09:44:09Z 置信度 0.64
• CRISPR-Cas9 Mediated Gene Editing in Tilapia: Enhancing Growth Rates and Disease Resistance
  CRISPR-Cas9 mediated gene editing has emerged as a transformative tool in aquaculture, offering precise genetic modifications to improve key traits in fish species such as tilapia. This study explores the potential of CRISPR-Cas9 technology to enhance growth r…
  crossref Chen Liang, Yi Lu, Rudi Mai 2024-11-15T06:07:33Z 置信度 0.76
• Induced Pluripotent Stem Cells and CRISPR-Cas9 Gene-Editing on Transthyretin Amyloid Cardiomyopathy
  Transthyretin amyloid cardiomyopathy is a fataldisease of the myocardium causing a protein buildup of Transthyretin. Over 120,000 people in the United States suffer from transthyretin amyloid cardiomyopathy, and half of those diagnosed will die within four yea…
  crossref Caden L. Reedy 2025-01-30T11:00:16Z 置信度 0.82 biologybiotechnologycancersynthetic biologycell biology
• CRISPR and Fanzor in sickle cell disease: current progress and future prospects.
  Advancements in genome editing have established a new frontier for the treatment of various genetic diseases, including sickle cell disease (SCD). SCD, the most prevalent monogenic blood disorder, causes severe pain, organ damage, and reduced life expectancy. …
  europepmc 2026 置信度 0.87
• A self-iterative orthogonal base-editing platform enables multiplex N-to-N diversification and genome-scale functional screening in Escherichia coli.
  Base editing enables precise genome modification without double-strand breaks but remains limited by narrow editing windows, DNA repair pathway biases, and restricted nucleotide diversity. Here, we report MUTATOR, a MUlTiplexAble and self-iTerative ORthogonal …
  europepmc 2026 置信度 0.87
• Genome Editing in Root and Tuber Crop Development in Sub-Saharan Africa.
  Precision genome editing, particularly using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9), is advancing crop improvement by enabling targeted and efficient genetic modifications. Root and tuber crops suc…
  europepmc 2026 置信度 0.94 biotechnologybiological sciences not elsewhere classifiedbiologysustainabilitygenetics
• Targeting biofilm-related genes in a clinical methicillin-resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; isolate using CRISPR-Cas9 gene editing.
  Background Methicillin-Resistant Staphylococcus aureus (MRSA) is a major clinical challenge due to its biofilm-forming ability. Innovative therapeutic strategies are essential to prevent bacterial attachment and disrupt biofilm structures. This study investiga…
  europepmc 2026 置信度 0.87
• CRISPR-Cas9-producing probiotic bacteria for editing NOX2/gp91&lt;sup&gt;phox&lt;/sup&gt; and treating inflammatory bowel disease.
  The primary pathogenic mechanism of inflammatory bowel disease (IBD) involves elevated levels of reactive oxide species (ROS) in the gut, leading to oxidative stress and damage to the intestinal barrier function. We engineered a non-pathogenic bacterial strain…
  europepmc 2026 置信度 0.87
• CRISPR-driven strategies to disrupt methicillin-resistant &lt;i&gt;Staphylococcus aureus&lt;/i&gt; biofilms: a review.
  Methicillin-resistant Staphylococcus aureus exhibits heightened tolerance to antimicrobial therapy through restricted drug penetration, extracellular polymeric substance-mediated protection, metabolic heterogeneity, and persister cell formation, limiting the e…
  europepmc 2026 置信度 0.87
• Advances in gene editing for legume improvement: technologies, progress, and prospects.
  Legumes are among the most important protein-rich crops in global agri-food systems. To meet the rising protein demand of a growing population, significant efforts are underway to enhance legume yield, nutritional quality, and resilience to environmental stres…
  europepmc 2026 置信度 0.87
• CRISPR/Cas9-Mediated Mutagenesis of OsERF94 Enhances Pre-Harvest Sprouting in Rice.
  Pre-harvest sprouting (PHS), where seeds germinate on panicles before harvest under humid conditions, is a serious global issue in cereal crop production, including rice. Fine-mapping of the previously reported chromosome 4 locus identified OsERF94 as a strong…
  europepmc 2026 置信度 0.87
• Reconstructing the complex architecture of the genome with molecular scissors: applying genome editing technology in precision medicine.
  Our past, present, and future are governed by the grand design of the genetic blueprint, which was mapped more than two decades back. While the eloquent design of our genome results from millions of years of evolution and has reached a near-perfect stage, unwa…
  europepmc 2026 置信度 0.87
• Genome editing of susceptibility gene &lt;i&gt;StDND2&lt;/i&gt; enhances &lt;i&gt;Phytophthora&lt;/i&gt; resistance in &lt;i&gt;Solanum tuberosum&lt;/i&gt;.
  Potato ( Solanum tuberosum L.) cultivation is severely constrained by multiple pathogens, among which late blight caused by the oomycete P. infestans remains the most destructive disease. Developing pathogen-resistant cultivars can enhance productivity and red…
  europepmc 2026 置信度 0.87
• From recalcitrance to precision: a robust regeneration, transformation and targeted gene editing framework in &lt;i&gt;Cajanus cajan&lt;/i&gt;.
  Pigeonpea ( Cajanus cajan (L.) Millsp.; 2n = 2× = 22) is a drought-tolerant perennial grain legume commonly cultivated in India's rain-fed and dry land zones, and it is a remarkable natural source of minerals and protein worldwide. Despite decades of research,…
  europepmc 2026 置信度 0.87
• Clinical Trial Landscape of Gene-Edited Autologous Hematopoietic Stem Cells for Hemoglobinopathies and Immunodeficiencies.
  Allogeneic hematopoietic cell transplantation (HCT) has been used for decades to treat certain malignant and non-malignant hematological conditions, but challenges remain. Increased understanding of disease mechanisms and recent developments in genome editing …
  europepmc 2026 置信度 0.87
• A genome sequence and efficient CRISPR/Cas9 gene editing tools for the solanaceous specialist pest Tetranychus evansi.
  Tetranychus evansi is an invasive spider mite pest of solanaceous crops worldwide. Its rapid global spread and ability to develop acaricide resistance highlight the need for robust genomic resources and functional genetic tools for custom control strategies. H…
  europepmc 2026 置信度 0.87
• Loss-of-function of MIR172b and prime editing of SNB reveal a regulatory module underlying cleistogamy in rice.
  Cleistogamy, or self-fertilization of a closed flower, can limit unintended gene flow and may contribute to varietal purity in rice (Oryza sativa), with potential value for transgene containment. We previously identified the natural cleistogamous mutant lodicu…
  europepmc 2026 置信度 0.87
• Multilayer regulation of CRISPR systems: integrating anti-CRISPR proteins, CRISPRi/a, and quorum sensing networks.
  The CRISPR-Cas system has evolved into a highly efficient platform for genome editing and programmable gene regulation, demonstrating broad application potential in microbiology, biotechnology, and medicine. However, traditional CRISPR tools typically rely on …
  europepmc 2026 置信度 0.94 computer sciencebiologysynthetic biologycomputational biologygenetics
• Advances in CRISPR-Cas for Diagnosis and Treatment of &lt;i&gt;Klebsiella pneumoniae&lt;/i&gt;.
  Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a significant pathogen for both hospital-acquired and community-acquired infections, characterized by its strong epidemic potential and high mortality rate, posing a severe threat to global public health. CR…
  europepmc 2026 置信度 0.87
• Programmable nanocarriers for precision plant engineering: converging nanotechnology, CRISPR, and next-generation breeding.
  The convergence of nanotechnology and genome editing in plant sciences is redefining modern precision breeding through efficient, transgene free, tissue culture independent pathways for genetic improvement in crops. Conventional breeding and transgenic tools a…
  europepmc 2026 置信度 0.94 biologymedicinebiotechnologycomputational biologyengineering
• High-fidelity genome and prime editing enabled by the AI-designed openCRISPR-1.
  Background RNA-guided nucleases such as CRISPR-Cas9 systems have revolutionized genome engineering by enabling programmable DNA modifications. Although structure-guided and evolution-derived high-fidelity Cas9 variants improve target specificity, they often co…
  europepmc 2026 置信度 0.87
• Targeted mutagenesis and base editing using engineered &lt;i&gt;Brevibacillus laterosporus&lt;/i&gt; Cas9 with expanded target scope in rice.
  The CRISPR/Cas9 system is now widely used for precise genome editing in many crop species, allowing targeted mutagenesis and precise base substitution. While CRISPR/Cas9-mediated genome editing is highly accurate, a limitation of this technology is the require…
  europepmc 2026 置信度 0.87
• Genome Editing of a Carotenogenic Gene for Lycopene Enhancement Increases Heavy Metal Stress Susceptibility in Tomato (Solanum lycopersicum L.).
  Improving the nutritional quality and abiotic stress tolerance of crop plants is essential for sustainable agriculture and global food security. Recent advances in genome editing, particularly the CRISPR/Cas9 system, have enabled precise modification of metabo…
  europepmc 2026 置信度 0.87
• Ribonucleic acid and gene therapies in cardiovascular disease: clinical applications, delivery challenges and emerging precision tools.
  Cardiovascular diseases remain a leading cause of global mortality despite advancements in pharmacotherapies, with current treatments facing challenges related to efficacy, tolerability and patient adherence. In response, advanced therapies, such as RNA and ge…
  europepmc 2026 置信度 0.87
• From QTL mapping to genome editing: advances and integrated strategies for improving aluminum tolerance in crops.
  Aluminum (Al) toxicity in acidic soils remains one of the most serious constraints on global crop production, limiting productivity across nearly one-third of the world's potentially arable land. As pressure grows to cultivate marginal lands under climate chan…
  europepmc 2026 置信度 0.87
• An evolutionary genomic perspective on preterm birth, genome editing, and pregnancy in the human species.
  The processes of labor and birth have a complex evolutionary history, with substantial variation among species showing differences in gestational length, offspring number, anatomy, and rates of fetal development. Understanding the genomic basis of pregnancy is…
  europepmc 2026 置信度 0.87
• Cis-regulatory elements in CAMTA-mediated stress signalling: mechanisms and prospects for CRISPR-based crop improvement.
  Enhancements in crop resilience strategies that maintain production are essential to address the challenges posed by climate change and increasing food consumption. Calcium-dependent signaling networks are essential for plant responses to abiotic and biotic st…
  europepmc 2026 置信度 0.87
• Genome-based taxonomy and provirus identification in &lt;i&gt;Halococcus&lt;/i&gt; from hypersaline environments.
  Halococcus species are widely distributed in hypersaline environments and possess unusual cell wall structures. However, their genomic diversity, antiviral defenses, and interactions with viruses remain poorly understood. Here, we sequenced and analyzed the ge…
  europepmc 2026 置信度 0.87
• Bacteriophage therapy against multidrug resistant bacterial infections demonstrates clinical advances and engineering innovations between 2020-2026.
  Purpose The global crisis of antimicrobial resistance has reached critical levels, with multi-drug resistant (MDR) bacterial pathogens threatening to render conventional antibiotics ineffective. This mini review synthesizes recent evidence from 2020 to 2026 on…
  europepmc 2026 置信度 0.87
• Corrigendum to "A virus-like particle candidate vaccine based on CRISPR/Cas9 gene editing technology elicits broad-spectrum protection against SARS-CoV-2" [Antivir. Res. 225 (2024) 105854].
  europepmc 2026 置信度 0.76
• CRISPR-based next-generation molecular diagnostics for bone infection.
  Bone and joint infections, notably osteomyelitis and periprosthetic joint infections, present a profound clinical challenge characterized by severe morbidity, prolonged hospitalizations, and substantial healthcare costs. Effective management is persistently hi…
  europepmc 2026 置信度 0.87
• Phage enabled precision drug delivery: dual function platforms for therapeutics and genetic cargo transport.
  The rising concern of antimicrobial resistance, coupled with the continually challenging management of complicated diseases such as cancer, has provided momentum toward precision molecular medicine. This review provides an overview of bacteriophage enabled str…
  europepmc 2026 置信度 0.87
• Editorial for Special Issue on Gene Therapy of Rare Diseases.
  A rare disease is a condition that affects only a small portion of the population [...].
  europepmc 2026 置信度 0.82
• Engraftment Outcome of CRISPR/Cas9-Edited Hematopoietic Stem Cells for Genetic Diseases: A Systematic Review and Meta-Analysis of Preclinical Evidence.
  Background CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9)-based gene editing represents a promising frontier for treating monogenic hematologic disorders. Several preclinical studies have demonstrated th…
  europepmc 2026 置信度 0.87
• CRISPR/Cas9 gene editing
  AccessScience is an authoritative and dynamic online resource that contains incisively written, high-quality educational material covering all major scientific disciplines. An acclaimed gateway to scientific knowledge, AccessScience is continually expanding th…
  crossref 2017-02-27T18:55:12Z 置信度 0.70
• Tests of Robustness in Peer Review
  Purpose: The purpose of this dissertation is to investigate the feasibility of using tests of robustness in peer review. This study involved selecting three high-impact papers which featured open data and utilized bioinformatic analyses but provided no source …
  datacite Leipzig,Jeremy 2021 置信度 0.76 BioinformaticsMetadataPeer reviewReproducibilityReproducible research
• Investigating the Potential of Proso Millet (Panicum miliaceum L.) as a Functional Food
  Abstract Proso millet (Panicum miliaceum L.) is among the earliest domesticated millet species and serves as a vital staple food for populations living in arid and semi-arid regions worldwide. This review highlights its rich nutritional profile, including high…
  datacite Tahere Razzaghi1, Fatemeh Naderi2, Maryam Salami1,3*, Gholamreza Askari4, Najmeh Salehi5 2025 置信度 0.77 medicinebiotechnologybiologybiochemistrypharmacology