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• Global Genetic Engineering Market 2024 To 2033
  Genetic Engineering Market Size, Trends and Insights By Product (Biochemical, Genetic Markers), By Devices (PCR, Gene Gun, Gel Assemblies), By Techniques (Gene Splicing, Artificial Selection, Recombinant DNA Technology, Synthesizing DNA, Gene Editing, Other), …
  datacite Sirsat, Nitin 2024 置信度 0.79 Genetic Engineering MarketGenetic Engineering Market SizeGenetic Engineering Market ShareGenetic Engineering Market TrendsGenetic Engineering Market Report
• Automated genome editing of induced pluripotent stem cells using the CRISPR/Cas9 method
  The discovery of the CRISPR/Cas9 system in 2012 has opened up new possibilities for targeted, inexpensive, and reproducible genetic modification of human cells. One of these possibilities is the targeted modification of induced pluripotent stem cells (iPS) for…
  datacite Nießing, Bastian 2024 置信度 0.76 Hochschulschriftgenome editing , CRISPR/Cas9 , automation , StemCellFactory , induced pluripotent stem cells , iPS cells
• Building a bridge between biophysics and neurobiology: A synergic approach to develop Alzheimer’s disease translational models and research tools
  Alzheimer's disease (AD) is the most prevalent neurodegenerative disorder, accounting for 60-70% of dementia cases diagnosed worldwide. The impact of AD is rapidly increasing, with deaths more than doubling from 2000 to 2021. As populations age, especially in …
  datacite Gonzalez Diaz, Alicia 2024 置信度 0.79 Amyloid-βiPSCOligomersPhase separationPreclinical models
• УСПЕХИ ГЕНОМНОГО РЕДАКТИРОВАНИЯ КАРТОФЕЛЯ
  Эффективность и точность подходов редактирования генома растений совершенствуются с каждым годом, благодаря чему значительно расширяются возможности улучшения различных характеристик сельскохозяйственных культур. Картофель – важнейшая овощная и техническая кул…
  datacite Дегтярёва, Влада, Долгов, Сергей, Мирошниченко, Дмитрий 2024 置信度 0.75 genome editingpotatoCRISPR/Cas9TALENгеномное редактирование
• Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system
  This repository contains extended and underlying data for "Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system". Modaffari et al., 2024.
  datacite Modaffari, Domenico 2024 置信度 0.75 Cell biologyMicrobiologyFOS: Biological sciences
• Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system
  This repository contains extended and underlying data for "Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system". Modaffari et al., 2024.
  datacite Modaffari, Domenico 2024 置信度 0.75 Cell biologyMicrobiologyFOS: Biological sciences
• Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system
  This repository contains extended and underlying data for "Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system". Modaffari et al., 2024.
  datacite Modaffari, Domenico 2024 置信度 0.75 Cell biologyMicrobiologyFOS: Biological sciences
• DeepFM-Crispr: Prediction of CRISPR On-Target Effects via Deep Learning
  Since the advent of CRISPR-Cas9, a groundbreaking gene-editing technology that enables precise genomic modifications via a short RNA guide sequence, there has been a marked increase in the accessibility and application of this technology across various fields.…
  datacite Bao, Condy, Liu, Fuxiao 2024 置信度 0.79 Quantitative Methods (q-bio.QM)Artificial Intelligence (cs.AI)Machine Learning (cs.LG)FOS: Biological sciencesFOS: Biological sciences
• JosefAlbers/Phi-3-Vision-MLX: Phi-3-MLX: Language and Vision Models for Apple Silicon
  Phi-3-MLX is a versatile AI framework that leverages both the Phi-3-Vision multimodal model and the recently updated (July 2, 2024) Phi-3-Mini-128K language model, optimized for Apple Silicon using the MLX framework. This project provides an easy-to-use interf…
  datacite JosefAlbers, Rémi 2024 置信度 0.72
• Versatile electroporation protocols enable reproducible CRISPR-RNP delivery across multiple primary mouse cells of the hematopoietic lineage
  Genetic engineering of primary hematopoietic cells is essential for mechanistic immunology studies, however the development of cell-based therapies yet remains constrained by two major factors: the fragility of many primary lineages and challenges of viral del…
  preprints 2026 置信度 0.81
• The Genetic Revolution in Cardiometabolic Disease: A Review of Novel RNA and Gene‐Editing Therapies Targeting APOC3, ANGPTL3, and Lp(a)
  Background: /Objectives: Despite the success of statin and PCSK9 inhibitor therapies, significant residual cardiovascular risk persists, driven by atherogenic triglyceride-rich lipoproteins (TRLs) and the independent, causal risk factor, lipoprotein(a) [Lp(a)]…
  preprints 2025 置信度 0.81
• WormTagDB: A Systematic Survey of Endogenously Tagged Proteins in C. elegans and Roadmap Towards the Tagged Proteome
  Endogenous protein tagging in Caenorhabditis elegans enables direct visualization and manipulation of proteins in vivo, providing native readouts of expression, localization, and dynamics. No coordinated effort currently exists to comprehensively tag proteins …
  preprints 2025 置信度 0.81
• Engineering quantitative root disease resistance in barley by targeting conserved SCAR susceptibility genes without compromising seed yield or mycorrhizal symbiosis
  Durable resistance to soil-borne pathogens remains elusive in cereals, partly because susceptibility (S) genes that facilitate root infection have not been identified in monocots. In the model legume Medicago truncatula , the SCAR/WAVE complex member MtAPI fun…
  preprints 2026 置信度 0.81
• Generating long deletions across the genome with pooled paired prime editing screens
  Engineered deletions are a powerful probe for studying genome architecture, function, and regulation. Yet, the lack of effective methods to create them in large numbers and at multi-kilobase scale has rendered interrogating all the gigabases of human DNA elusi…
  preprints 2025 置信度 0.81
• Sleeping ORANGE: A Transposase-Enhanced CRISPR Approach to Boost Endogenous Protein Tagging Efficiency
  Background Investigating the subcellular distribution of proteins is crucial for understanding complex cell behaviours and disease mechanisms, and fluorescence microscopy has become a key tool for visualising protein localisation. Endogenous protein tagging, w…
  preprints 2025 置信度 0.81
• Stromal Prostaglandin is a Dominant Spatial Regulator of Cell-fate Plasticity in Colorectal Cancer
  Abstract Colorectal cancer (CRC) tumours with high stromal content have a worse outcome, but the mechanisms governing this are unclear. Using high-throughput single-cell perturbation analysis of CRC patient-derived organoids (PDOs) and cancer-associated fibrob…
  preprints 2026 置信度 0.81
• A tRNA-gRNA multiplexing system for CRISPR genome editing in <i>Marchantia polymorpha</i>
  The liverwort Marchantia polymorpha is a widely used model organism for studying land plant biology, which has also proven to be a promising testbed for bioengineering. CRISPR/Cas9 technology has emerged as a transformative tool for precise genome modification…
  preprints 2025 置信度 0.81
• Oxidative Stress Susceptibility, Complement Dysregulation, and Metabolic Reprogramming in CFH Y402H Patient-Derived Choriocapillaris Endothelial Cells
  Abstract Age-related macular degeneration (AMD) is a leading cause of central vision loss. Immunofluorescence and gene expression studies in human donor eyes have shown that choriocapillaris endothelial cells (CECs) are lost before retinal pigment epithelium (…
  preprints 2026 置信度 0.86 biologyneurosciencecell biologymedicinecancer
• SUBCELLULAR FUNCTIONS OF <i>UBE3A</i> ISOFORMS DRIVE SYNAPTIC DYSFUNCTION IN ANGELMAN SYNDROME
  ABSTRACT Genetic defects of the gene encoding the ubiquitin ligase UBE3A cause a severe neurodevelopmental disorder, the Angelman syndrome (AS). The pathophysiology of AS remains unclear, hindering the development of effective therapies. Using AS animal models…
  preprints 2026 置信度 0.81
• Cutting Through the Artifacts: Dissecting gRNA Impurities with FUSS-seq
  CRISPR-based therapeutics rely on guide RNAs (gRNAs) and the Cas9 endonuclease for precise gene editing. Ensuring gRNA purity and base-level sequence integrity is essential for clinical translation. While industry-standard practice relies on liquid chromatogra…
  preprints 2025 置信度 0.81
• CRISMER: A Transformer-based Interpretable Deep Learning Approach for Genome-wide CRISPR-Cas9 Off-Target Prediction and Optimization
  CRISPR–Cas9 gene editing holds transformative promise for genetic therapies, but is hindered by off-target effects that undermine its precision and safety. To address this, we developed CRISMER, a hybrid deep-learning architecture that uses multi-branch convol…
  preprints 2025 置信度 0.81
• Gene Editing and Aging: A Bibliometric Analysis of Global Trends and Frontier Themes (2015-2024)
  Objective The accumulation of DNA damage and mutations is a key contributor to aging. Recent studies have shown that disrupting the Beclin 1-BCL2 autophagy regulatory complex through gene editing can extend lifespan in mice. The precise application of gene edi…
  preprints 2025 置信度 0.86 cancermedicinescience policymicrobiologydata science
• Human iPSC-derived liver organoids model multicellular tissue responses and therapeutic rescue in Wolman disease
  Wolman disease (WD), the severe infantile form of lysosomal acid lipase deficiency, is a rare metabolic disorder caused by inactivating mutations in the LIPA gene. Although WD is characterized by profound hepatic dysfunction, experimental human systems capable…
  preprints 2025 置信度 0.81
• Supercoiling twists Cas9 off-target discrimination when nicking and cleaving
  Programmed with an RNA guide, Cas9 nuclease directs double-strand DNA cleavage via its two nuclease domains. However, Cas9 sometimes falsely identifies DNA targets by binding and cleaving DNA that does not match its PAM and guide RNA sequence requirements. Cas…
  preprints 2026 置信度 0.81
• Retinal organoids mirror CRISPR/Cas9 gene editing efficiency observed in vivo
  Human retinal organoids are in vitro 3D structures that recapitulate key molecular and structural characteristics of the in vivo retina. They include the presence of all essential retinal cell types including photoreceptors, making them relevant models for pre…
  preprints 2024 置信度 0.81
• A mechanistic free-energy model explains and predicts sequence- and context-dependent CRISPR–Cas9 activity
  Accurate prediction of CRISPR-based gene editing remains challenging since existing models often fail to generalize across experimental and cellular contexts. We introduce CRISPRzip, a mechanistic kinetic model that quantitatively links nucleotide sequence and…
  preprints 2025 置信度 0.81
• Ovothiol A mediates singlet oxygen resistance and acclimation in Chlamydomonas
  Light is essential for photosynthetic organisms, but excess light can generate toxic levels of reactive oxygen species (ROS). To neutralize these ROS, plants and algae produce a variety of antioxidants like carotenoids, tocopherols, and glutathione. However, t…
  preprints 2026 置信度 0.81
• Metabolite import via SLC33A1 enables ATF6 activation by endoplasmic reticulum stress
  The transcription factor ATF6 has a central role in adapting mammalian cells to endoplasmic reticulum (ER) stress via the Unfolded Protein Response (UPR). This has driven efforts to identify modulators of ATF6 signalling. Here, an unbiased genome-wide CRISPR-C…
  preprints 2025 置信度 0.81
• Refined CRISPR/Cas9 genome editing in pea aphids uncovers the essential roles of <i>Laccase2</i> in overwintering egg adaptation
  The production of overwintering eggs is a critical adaptation for winter survival among many insects. Melanization contributes to eggshell pigmentation and hardening, consequently enhancing resistance to environmental stress. The complex life cycle of the pea …
  preprints 2024 置信度 0.81
• A CROP-seq screen of histone modifying enzymes reveals histone demethylase Kdm5c regulates inflammatory macrophage activation
  Background Macrophages adopt activation states along a spectrum from pro- to anti-inflammatory, enabling appropriate responses to pathogens and environmental cues. Dysregulated inflammatory macrophage activation contributes to diseases including sepsis, rheuma…
  preprints 2025 置信度 0.81
• Initiation of asexual reproduction by the AP2/ERF gene <i>GEMMIFER</i> in <i>Marchantia polymorpha</i>
  Summary Plants can propagate their own clones through asexual reproduction. Genetic and hormonal factors regulating asexual reproduction have begun to be elucidated in the liverwort Marchantia polymorpha , which produce asexual propagules called gemmae within …
  preprints 2026 置信度 0.81
• Design of Theophylline Binding Guide RNA for Allosteric Regulation of CRISPR/Cas9 System
  The possibility of CRISPR/Cas gene editing system regulation is an important point for creation of effective molecular biological instrument. Allosteric regulation at the level of guide RNAs is one of possible approach to regulate gene editing system. In this …
  preprints 2024 置信度 0.81
• Optimization of CRISPR/Cas9 Gene Editing System in Sheep Oocytes via Microinjection
  The CRISPR/Cas9 system has become a powerful tool for molecular design breeding in livestock such as sheep. However, the efficiency of the Cas9 system combined with zygote microinjection remains suboptimal. In this study, mature sheep oocytes were used for mic…
  preprints 2024 置信度 0.81
• A compendium of nonredundant short Polymerase III promoters for CRISPR applications
  Multiplex genome editing via CRISPR enables simultaneous gene knockout, activation, and repression, but current toolkits often rely on repetitive and lengthy Pol III promoters, limiting construct diversity and genetic stability. Here, we present a diverse coll…
  preprints 2024 置信度 0.81
• <i>Pseudomonas putida</i> JM37 as a novel bacterial chassis for ethylene glycol upcycling
  Ethylene glycol (EG), one of the main monomers of polyethylene terephthalate (PET), is an attractive target for microbial upcycling. Despite this interest, there is a limited number of described organisms that can efficiently metabolise EG. Here, we report the…
  preprints 2025 置信度 0.81
• Reciprocal interactions between EMT and BMP signalling drive collective cell invasion
  During collective cell invasion, epithelial-to-mesenchymal transition (EMT) and morphogen signalling-mediated cell fate specification are traditionally viewed as a linear cascade: morphogens drive cell fates that activate EMT programs. Here, we uncover recipro…
  preprints 2025 置信度 0.81
• A self-limiting Sterile Insect Technique alternative for <i>Ceratitis capitata</i>
  Genetic biocontrol systems have broad applications in population control of insects implicated in both disease spread and food security. In this study we establish and characterise a novel split-CRISPR/Cas9 system we term Sex Conversion Induced by CRISPR (SCIC…
  preprints 2024 置信度 0.81
• Improved gene editing and fluorescent-protein tagging in Aspergillus nidulans using a Golden Gate-based CRISPR-Cas9 plasmid system
  CRISPR-Cas9 systems can be used for precise genome editing in filamentous fungi, including Aspergillus nidulans . However, current CRISPR-Cas9 systems for A. nidulans rely on relatively complex or multi-step cloning methods to build a plasmid expressing both C…
  preprints 2024 置信度 0.81
• Cardiac Applications of CRISPR/AAV-Mediated Precise Genome Editing
  The ability to efficiently make precise genome edits in somatic tissues will have profound implications for gene therapy and basic science. CRISPR/Cas9 mediated homology-directed repair (HDR) is one approach that is commonly used to achieve precise and efficie…
  preprints 2024 置信度 0.81
• Efficacy of CRISPR-Cas9 Gene Editing for Targeting KRAS Mutations in Pancreatic Cancer: A systematic review
  Abstract Background Pancreatic cancer is one of the main causes of cancer-related deaths, especially pancreatic ductal adenocarcinoma and it’s characterized by a poor prognosis. The KRAS gene mutation is prevalent in about 85% of pancreatic cancer cases which …
  preprints 2024 置信度 0.81
• Application of CRISPR/Cas9 Technology in Rice Germplasm Innovation and Genetic Improvement
  Improving the efficiency of germplasm innovation has always been the aim of rice breeders. Tra-ditional hybrid breeding methods for variety selection rarely meet the practical needs of rice pro-duction. The emergence of genome editing technologies, such as CRI…
  preprints 2024 置信度 0.81
• A Key Role for <i>S-</i> Nitrosylation in Immune Regulation and Development in the Liverwort <i>Marchantia polymorpha</i>
  Nitric oxide (NO) is an important signaling molecule in flowering plant immunity. It rapidly accumulates in response to pathogen perception. In addition to it’s direct response to microbes, NO controls a range of defence responses primarily through S -nitrosyl…
  preprints 2025 置信度 0.81
• Efficient mutagenesis of maize inbreds using biolistics, multiplex CRISPR/Cas9 editing, and Indel-Selective PCR
  CRISPR/Cas9 based genome editing has advanced our understanding of a myriad of important biological phenomena. Important challenges to multiplex genome editing in maize include assembly of large complex DNA constructs, few genotypes with efficient transformati…
  preprints 2024 置信度 0.81
• Haplotype editing with CRISPR/Cas9 as a therapeutic approach for dominant-negative missense mutations in <i>NEFL</i>
  ABSTRACT Inactivation of disease alleles by allele-specific editing is a promising approach to treat dominant-negative genetic disorders, provided the causative gene is haplo-sufficient. We previously edited a dominant NEFL missense mutation with inactivating …
  preprints 2024 置信度 0.81
• Optimizing Genome Editing in Bovine Cells: A Comparative Study of Cas9 Variants and CRISPR Delivery Methods
  To assist in the establishment of an efficient CRISPR/Cas9 gene editing workflow in bovine cells, we compared the efficiency of four Streptococcus pyogenes Cas9 (SpCas9) nuclease variants (produced in-house or commercially) and two different Cas9/sgRNA ribonuc…
  preprints 2024 置信度 0.81
• Accurate prediction of CRISPR editing outcomes in somatic cell lines and zygote with few-shot learning
  The CRISPR-Cas system has revolutionized gene editing, while its outcome prediction remains unsatisfactory, especially in new cell states due to their distinct DNA repair preferences. In this study, we introduce inDecay, a flexible system for predicting CRISPR…
  preprints 2024 置信度 0.81
• Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins
  Programmable epigenome editors modify gene expression in mammalian cells by altering the local chromatin environment at target loci without inducing DNA breaks. However, the large size of CRISPR-based epigenome editors poses a challenge to their broad use in b…
  preprints 2024 置信度 0.81
• EASY-CRISPR: a toolbox for high-throughput single-step custom genetic editing in bacteria
  Targeted gene editing can be achieved using CRISPR/Cas9-assisted recombineering. However, high-efficiency editing requires careful optimization for each locus to be modified which can be tedious and time-consuming. In this work, we developed a simple, fast and…
  preprints 2025 置信度 0.86 biologysynthetic biologycomputational biologygeneticsmodular design
• New alleles of Arabidopsis <i>BIK1</i> reinforce its predominant role in pattern-triggered immunity and caution interpretations of other reported functions
  The receptor-like cytoplasmic kinase BIK1 and its close homolog PBL1 have been widely recognized as central components of plant immunity. However, most genetic studies of BIK1 and PBL1 functions were carried out with single T-DNA insertional mutant alleles. So…
  preprints 2025 置信度 0.81
• Successful delivery of CRISPR-Cas9 with a baculovirus vector for insect brain targets
  Summary CRISPR-Cas9 (clustered, regularly interspaced, short palindromic repeats with CRISPR-associated protein 9) is a powerful, versatile, and cost-effective molecular tool that can be used for genetic engineering purposes and beyond 1 and is especially suit…
  preprints 2024 置信度 0.81
• KOnezumi-AID: A Software to Automate the Design of Multiplex Knockouts Using Target-AID
  With the groundbreaking advancements in genome editing technologies, particularly CRISPR-Cas9, creating knockout mutants has become highly efficient. However, the CRISPR-Cas9 system introduces DNA double-strand breaks, which can increase the risk of chromosoma…
  preprints 2024 置信度 0.81
• Facilitating Gene Editing in Human Lymphoma Cells Using Murine Ecotropic γ-Retroviruses
  Genetic modifications using CRISPR-Cas9 have revolutionized cancer research and other pre-clinical studies. Exceptionally, these efficient tools are inadequate in a few disease models and cell lines due to the aberrant differentiation states and the accumulati…
  preprints 2024 置信度 0.81
• Cas9-expressing cattle using all-in-one CRISPR/Cas9 for bovine genome editing
  Abstract Background Livestock, particularly cattle, are crucial for biotechnology fields, such as genetic breeding, infectious diseases, bioreactors, and specific disease models. However, genetic engineering in cattle has lagged due to long gestation periods, …
  preprints 2024 置信度 0.81
• Targeted H3K9 acetylation at lncSox1 promoter by cell type-specific epigenome editing promotes intermediate progenitor proliferation in developing mouse cortex
  SUMMARY The distribution and level of epigenetic (chromatin) marks have implications for differential regulatory effects at specific gene loci. Herein, we applied a protocol which combines in vivo electroporation and a CRISPR-dead (d)Cas9 system to probe and e…
  preprints 2024 置信度 0.81
• Rapid degradation of 6 class I HDAC complexes reveals minimal functional overlap between complexes
  The class 1 HDACs 1, 2 and 3 form seven families of distinct large multiprotein complexes that regulate gene expression via deacetylation of lysines in histone tails. The degree of redundancy and functional overlap between complexes and their primary gene targ…
  preprints 2025 置信度 0.86 biologycomputational biologygeneticssynthetic biologyq-bio.gn
• Chemically-inducible CRISPR/Cas9 circuits for ultra-high dynamic range gene perturbation
  CRISPR/Cas9 technologies provide unique capabilities for modeling disease and understanding gene-to-phenotype connections. In cultured cells, chemical-mediated control of Cas9 activity can limit off-target effects and enable mechanistic study of essential gene…
  preprints 2024 置信度 0.81
• Coupling CRISPR Scanning with Targeted Chromatin Accessibility Profiling using a Double-Stranded DNA Deaminase
  Genome editing enables sequence-function profiling of endogenous cis-regulatory elements, driving understanding of their mechanisms and the development of gene therapies. However, these approaches cannot be combined with direct scalable readouts of chromatin s…
  preprints 2024 置信度 0.81
• CRISPR-CAS9 MEDIATED EDITING OF THE <i>LACZ</i> GENE FOR OPTIMIZING LIPASE PRODUCTION AND CHARACTERIZATION IN ENGINEERED <i>ESCHERICHIA COLI</i> USING POTATO PEEL AS A GLUCOSE SOURCE
  ABSTRACT Improper disposal of potato peels harms the environment, wasting nutrient-rich resources that could be used for beneficial enzyme production like lipase. This study aimed to edit the lacZ gene in Escherichia coli using utilizing the CRISPR Cas9 techno…
  preprints 2024 置信度 0.81
• StreptoCAD: An open-source software toolbox automating genome engineering workflows in streptomycetes
  Streptomycetes hold immense potential for discovering novel bioactive molecules for applications in medicine or sustainable agriculture. However, high-throughput exploration is hampered by the current Streptomyces genetic engineering methods that involve the m…
  preprints 2024 置信度 0.81
• A CRISPR/ <i>Sp</i> Cas9M-reporting system for efficient and rapid genome editing in <i>Caulobacter crescentus</i>
  As members of the α-proteobacteria group, Caulobacter crescentus and its relatives are known for their asymmetric life cycle and comprehensive applications in gene delivery, agricultural biotechnology, and the production of high-value compounds. However, genet…
  preprints 2024 置信度 0.81
• Pioneering Genome Editing in Parthenogenetic Stick Insects: CRISPR/Cas9-Mediated gene knockout in Medauroidea extradentata
  Abstract The parthenogenetic life cycle of the stick insect Medauroidea extradentata offers unique advantages for the generation of transgenic lines, as an isogenic and stable transgenic line can in principle be achieved already in the first generation. Howeve…
  preprints 2024 置信度 0.81
• Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells
  Verification of genome editing in human pluripotent stem cells (hPSCs), particularly in silent locus is desirable but challenging because it often requires complex and time-intensive lineage-specific or tissue-specific differentiation to induce their expressio…
  preprints 2024 置信度 0.81
• Application of a GRF-GIF chimera enhances plant regeneration for genome editing in tomato
  Genome editing has become a routine tool for functionally characterizing plant and animal genomes. However, stable genome editing in plants remains limited by the time- and labor- intensive process of generating transgenic plants, as well as by the efficient i…
  preprints 2024 置信度 0.81
• CRISPRoff epigenetic editing for programmable gene silencing in human cells without DNA breaks
  The advent of CRISPR-based technologies has enabled the rapid advancement of programmable gene manipulation in cells, tissues, and whole organisms. An emerging platform for targeted gene perturbation is epigenetic editing, the direct editing of chemical modifi…
  preprints 2024 置信度 0.81
• The Advancement and Applications of Prime Editing
  Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), an exceptionally potent genome-editing technique developed in 2012, is the ideal tool of the future for treating diseases by permanently correcting deleterious base mutations or disrupting dis…
  preprints 2024 置信度 0.86 nanotechnologybiologypharmacologygeneticsvariety
• The tryptophan-binding pockets of Arabidopsis AGO1 facilitate amplified RNA interference via SGS3
  ABSTRACT ARGONAUTE (AGO) proteins associate with small RNAs to form RNA-induced silencing complexes (RISCs). Arabidopsis AGO1 effects post-transcriptional silencing by microRNAs (miRNAs) and small interfering RNAs (siRNAs) and is necessary for siRNA amplificat…
  preprints 2025 置信度 0.72
• Translational reading frame determines the pathogenicity of C-terminal frameshift deletions in MeCP2: an alternative therapeutic approach
  Mutations in the MECP2 gene cause the severe neurological disorder Rett syndrome. A cluster of frameshift-causing C-terminal deletions (CTDs) lead to loss of ∼100 amino acids at the C-terminus of the MeCP2 protein, and account for approximately 10% of RTT-caus…
  preprints 2025 置信度 0.81
• Disruption of the SAGA CORE triggers collateral degradation of KAT2A
  SUMMARY The SAGA transcriptional co-activator complex regulates gene expression through histone acetylation at promoters, mediated by its histone acetyl transferase, KAT2A. While its structure and function have been extensively investigated, how the stability …
  preprints 2025 置信度 0.81
• Potential CRISPR/Cas9 Gene Editing Applications in Reversing Acquired Resistance to EGFR-TKIs due to Secondary and Tertiary Mutations to EGFR in NSCLC
  Lung cancer treatment has long been a major field of study in research due to the com-plexity and variability of cancer. Because drug resistance is a major hurdle for lung cancer treat-ment, in this article, we will review the epidermal growth factor receptor …
  preprints 2024 置信度 0.81
• <i>De novo</i> design of potent CRISPR-Cas13 inhibitors
  CRISPR-Cas systems are transformative tools for gene editing which can be tuned or controlled by anti-CRISPRs (Acrs) - phage derived inhibitors that regulate CRISPR-Cas activity. However, Acrs that are capable of inhibiting biotechnologically relevant CRISPR s…
  preprints 2024 置信度 0.81
• Awakening prime editing for precision engineering of probiotic <i>Escherichia coli</i> Nissle 1917
  CRISPR-Cas systems are transforming precision medicine with engineered probiotics as next-generation diagnostics and therapeutics. To promote human health and treat disease, engineering probiotic bacteria demands maximal versatility to enable non-natural funct…
  preprints 2024 置信度 0.81
• Unlocking The Potential and Myth of Personalized Vaccines, CRISPR-CAS9, and Bacteriotherapy, as Therapeutic Options for Cancer
  Recent advancements in cancer treatment, including targeted therapy, personalized vaccines, immunotherapy, and bacteriotherapy have shown promising potential in the curing of many kinds of cancer. The pursuit of innovative therapeutic approaches is impeded by …
  preprints 2024 置信度 0.81
• Engineered CRISPR-Base Editors as a Permanent Treatment for Familial Dysautonomia
  Familial dysautonomia (FD) is a fatal autosomal recessive sensory and autonomic neuropathy. FD is caused by a T-to-C point mutation in intron 20 of the Elongator acetyltransferase complex subunit 1 ( ELP1 ) gene, which results in tissue-specific skipping of ex…
  preprints 2024 置信度 0.81
• CRISPR associated enzymes are mislocalized to the cytoplasm in iPSC-derived neurons resulting in KRAB-specific degradation
  The use of CRISPR-associated enzymes in iPSC-derived neurons for precise gene targeting and high-throughput gene perturbation screens offers great potential but presents unique challenges compared to dividing cell lines. CRISPRi screens in iPSC-derived neurons…
  preprints 2024 置信度 0.81
• Enhanced <i>in vivo</i> gene knockout with undetectable off-targets using multiplexed Cas12a sgRNAs
  CRISPR nuclease-mediated gene knock-out is limited by suboptimal sgRNAs, inaccessible target sites, and silent mutations. Here, we present a Cas12a-based system that targets each gene with four sgRNAs to overcome these limitations, using Drosophila as a tracta…
  preprints 2024 置信度 0.81
• Nucleophagy removes cytotoxic trapped PARP1
  Poly (ADP-Ribose) Polymerase inhibitors (PARPi) induce cytotoxicity in homologous recombination repair (HRR)-deficient cancers by causing PARP1 to become trapped on chromatin, resulting in irreparable replication-associated DNA damage. Although increased clear…
  preprints 2025 置信度 0.81
• Structural basis for a dual-function type II-B CRISPR-Cas9
  Cas9 from Streptococcus pyogenes (SpCas9) revolutionized genome editing by enabling programmable DNA cleavage guided by an RNA. However, SpCas9 tolerates mismatches in the DNA-RNA duplex, which can lead to deleterious off-target editing. Here, we reveal that C…
  preprints 2024 置信度 0.81
• Engineering Adaptive Alleles for <i>Escherichia coli</i> Growth on Sucrose Using the EasyGuide CRISPR System
  Adaptive Laboratory Evolution (ALE) is a powerful approach for mining genetic data to engineer industrial microorganisms. This evolution-informed design requires robust genetic tools to incorporate the discovered alleles into target strains. Here, we introduce…
  preprints 2024 置信度 0.81
• Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction
  Single-cell transcriptomic and proteomic technologies enable molecular profiling of immune cells at scale but provide limited access to cellular phenotypes shaped by spatial organisation, organelle architecture and cytoskeletal remodelling. Here we present TGl…
  preprints 2026 置信度 0.81
• Single cell variation and rapid emergence of phenotypic heterogeneity in cell lines- a cautionary tale for devotees of CRISPR-Cas9
  The use and publication of research utilising CRISPR/cas9 in gene editing and knock outs (KOs) within cell lines is now widespread and has proved extremely powerful in the interrogation of gene function. However, the potential of experimental artefacts resulti…
  preprints 2024 置信度 0.81
• Hi-TARGET: A fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui
  Abstract Background Due to its ability to grow fast on CO 2 , CO and H 2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host for industrial biotechnology. In a circular carbon…
  preprints 2025 置信度 0.81
• Astrocytic modulation of population encoding in mouse visual cortex via GABA transporter 3 revealed by multiplexed CRISPR/Cas9 gene editing
  Astrocytes, which are increasingly recognized as pivotal constituents of brain circuits governing a wide range of functions, express GABA transporter 3 (Gat3), an astrocyte-specific GABA transporter responsible for maintenance of extra-synaptic GABA levels. He…
  preprints 2024 置信度 0.81
• Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency
  Plant gene editing technology has significantly advanced in recent years, thereby transforming both biotechnological research and agricultural practices. This review provides a comprehensive summary of recent advancements in this rapidly evolving field, showca…
  preprints 2024 置信度 0.81
• CRISPR/Cas9 alter NFYA binding site on CD44-regulating cis-element and control CD44 expression in breast cancer cells
  Abstract Background CRISPR/Cas9 is used for editing of non-coding sequence. This study tested the involvement of downstream cis-element in regulating CD44 expression and the possibility of using CRISPR/Cas9 system to manipulate the transcription factor binding…
  preprints 2024 置信度 0.81
• Defining Mechanistic Links Between the Non-Coding Variant rs17673553 in<i>CLEC16A</i>and Lupus Susceptibility
  Systemic lupus erythematosus (SLE) is a complex autoimmune disorder characterized by widespread inflammation and autoantibody production. Its development and progression involve genetic, epigenetic, and environmental factors. Although genome-wide association s…
  preprints 2024 置信度 0.81
• How Helpful May Be a CRISPR/Cas-Based System for Food Traceability?
  Crops obtained by genome editing (GE) technologies represent a notable and disruptive shift from genetically modified or transgenic plants, outpacing public perception and regulatory frame-works. Multiple aspects of GE are contributing to the advancement of mo…
  preprints 2024 置信度 0.81
• Ligase-mediated programmable genomic integration (L-PGI): an efficient site-specific gene editing system that overcomes the limitations of reverse transcriptase-based editing systems
  Summary Since their discovery, CRISPR/Cas9 systems have been repurposed for programmable targeted genomic editing. This has led to unprecedented advancement of gene editing for therapeutic benefit. Initial uses of CRISPR/Cas9 were focused on gene disruption vi…
  preprints 2024 置信度 0.81
• PETRA: Prime editing of transcribed regulatory elements to assay expression
  ABSTRACT Predicting how changes in human DNA sequence impact gene expression remains challenging. Here, we present PETRA ( P rime E diting of T ranscribed R egulatory elements to A ssay expression), a multiplexed genome editing method to quantify the effects o…
  preprints 2025 置信度 0.81
• CRISPR-GEM: A Novel Machine Learning Model for CRISPR Genetic Target Discovery and Evaluation
  CRISPR gene editing strategies are shaping cell therapies through precise and tunable control over gene expression. However, achieving reliable therapeutic effects with improved safety and efficacy requires informed target gene selection. This depends on a tho…
  preprints 2024 置信度 0.81
• CRISPR-Cas9/Cas12a Systems for efficient genome editing and large genomic fragment deletions in <i>Aspergillus niger</i>
  CRISPR technology has revolutionized fungal genetic engineering by accelerating the pace and expanding the feasible scope of experiments in this field. Among various CRISPR-Cas systems, Cas9 and Cas12a are widely used in genetic and metabolic engineering. In f…
  preprints 2024 置信度 0.81
• Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs
  ABSTRACT Nucleic acid nanostructures offer unique opportunities for biomedical applications due to their sequence-programmable structures and functions, which enable the design of complex responses to molecular cues. Control of the biological activity of thera…
  preprints 2024 置信度 0.81
• A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440
  Abstract Background Pseudomonas putida KT2440, a non-pathogenic soil bacterium, is a key platform strain in synthetic biology and industrial applications due to its robustness and metabolic versatility. Various systems have been developed for genome editing in…
  preprints 2024 置信度 0.81
• Gene editing tool-loaded biomimetic cationic vesicles with highly efficient bacterial internalization for in vivo eradication of pathogens
  Abstract In the post-COVID-19 era, drug-resistant bacterial infections emerge as one of major death causes, where multidrug-resistant Acinetobacter baumannii (MRAB) and drug-resistant Pseudomonas aeruginosa (DRPA) represent primary pathogens. However, the clas…
  preprints 2024 置信度 0.81
• Crackling Cloud: an event-driven, cloud-based CRISPR-Cas9 guide RNA design tool
  Gene editing has been revolutionised by the CRISPR-Cas9 technology. The versatility and ease-of-use of the technology far exceeds its predecessors, however, the selection of a high-quality guide RNA (gRNA) is critical to directing it to a target site. Selectin…
  preprints 2024 置信度 0.81
• Enhancement of CRISPR-Cas12a System through Universal Circular RNA Design
  Abstract The combination of isothermal amplification and CRISPR-Cas12a system is a valuable tool for detecting nucleic acids, especially in emergency situations or disease outbreaks. However, achieving compatibility between these technologies has been a challe…
  preprints 2024 置信度 0.81
• Emerging Strategies in Cartilage Repair and Joint Preservation
  Background: and Objectives. Cartilage repair remains a critical challenge in orthopaedic medicine due to the tissue&#039;s limited self-healing ability, contributing to degenerative joint conditions such as osteoarthritis (OA). In response, regenerative medici…
  preprints 2024 置信度 0.81
• FLP-15 functions through the GPCR NPR-3 to regulate local and global search behaviours in Caenorhabditis elegans
  Abstract The transition from well-fed to food-deprived conditions in C. elegans triggers a stereotypic exploration behaviour in C. elegans characterised by a temporal decrease in cumulative reorientations. We conducted a screen of neuropeptide mutants and iden…
  preprints 2025 置信度 0.77
• Molecular engineering and dynamic activation mechanism of a PAM-flexible compact CRISPR-Cas9
  Abstract Staphylococcus aureus Cas9 (SaCas9), which is smaller than the widely-used Streptococcus pyogenes Cas9 (SpCas9), has been harnessed for gene therapy using an adeno-associated virus vector. However, SaCas9 requires an NNGRRT (where N is any nucleotide …
  preprints 2024 置信度 0.81
• Mechanistic Insights into Dual-Active Liver and Blood-Stage Antiplasmodials
  The identification of novel antimalarials with activity against both the liver and blood stages of the parasite lifecycle would have the dual benefit of prophylactic and curative potential. However, one challenge of leveraging chemical hits from phenotypic scr…
  preprints 2025 置信度 0.81
• Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in <i>Plasmodium berghei</i>
  Malaria is caused by protozoan parasites of the genus Plasmodium and remains a global health concern. The parasite has a highly adaptable life cycle comprising successive rounds of asexual replication in a vertebrate host and sexual maturation in the mosquito …
  preprints 2024 置信度 0.81