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Plant regeneration and transformation remain significant bottlenecks towards the genetic improvement of most crop species by either genome editing or transgenic approaches. Recent research has therefore, transitioned from manual optimization of culture media a…
europepmc
2026
置信度 0.87
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Almost all protein-coding and long noncoding genes that are transcribed by RNA polymerase II employ cleavage and polyadenylation (CPA) for 3' end maturation of their nascent RNAs. More than 70% of human mRNA genes display alternative polyadenylation (APA), res…
europepmc
2026
置信度 0.87
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Medicinal plants are rich in bioactive constituents and are extensively utilized in the healthcare sector, holding significant value for both industrial and daily applications. In the context of economic development and escalating environmental challenges, the…
europepmc
2026
置信度 0.87
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Programmable genome editing technologies have reshaped the landscape of biomedical sciences, enabling the development of methods with great translational potential. CRISPR-Cas represents one of the most important and widely adopted genome editing tools, althou…
europepmc
2026
置信度 0.87
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Ischemic stroke (IS) is one of the most common neurological diseases worldwide and is caused by the blockage of cerebral blood vessels, leading to reduced blood flow and neuronal damage. Given the limitations of existing treatments, CRISPR gene-editing technol…
europepmc
2026
置信度 0.87
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The U12-dependent, or minor, spliceosome processes only 0.5% of human introns, and yet, it is known to profoundly influence gene expression and cellular signaling. The ZCRB1 protein is a core component of the U12 mono-snRNP, but its functional significance to …
europepmc
2026
置信度 0.87
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Myostatin gene (MSTN), a TGF-β superfamily member, negatively regulates skeletal muscle growth, positioning it as a prime genetic target for enhancing livestock meat production. This review synthesizes evidence from 1979 to 2025 on MSTN mutations across cattle…
europepmc
2026
置信度 0.87
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Background Limitations in genome editing of avian species, including chicken, due to the inaccessibility of one-cell zygotes, have led to the manipulation of primordial germ cells (PGCs) as the primary approach for generating genetically engineered birds. Alth…
europepmc
2026
置信度 0.87
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Parkinson's disease (PD) entails loss of substantia nigra dopamine (DA) neurons and α-synuclein pathology. Currently, no effective disease-modifying therapies have been developed. Human pluripotent stem cells (hPS cells) can generate DA neurons on scale, enabl…
europepmc
2026
置信度 0.87
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FRMD5 is a protein-coding gene on human chromosome 15q15.3 encoding a FERM domain-containing protein. Its mutations are linked to NEDEMA, a rare autosomal dominant neurodevelopmental disorder characterized by developmental delay, intellectual disability, ataxi…
europepmc
2026
置信度 0.87
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Background/objectives Despite the transformative potential of gene editing technologies, a systematic mapping of their translational trajectory from bench to bedside remains scarce. This bibliometric analysis aims to chart the bench-to-bedside evolution, ident…
europepmc
2026
置信度 0.87
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Globally, nearly one million species are currently threatened with extinction, highlighting the need for more efficient solutions to biological conservation. Genome editing, which allows for faster and more precise changes in genomes, is a promising technique …
europepmc
2026
置信度 0.87
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Plant secondary metabolites, including lignans, play essential roles in plant defense and adaptation, and their pharmacological properties are increasingly valued for human health. Lignans are dimers derived from the phenylpropanoid pathway whose biosynthesis …
europepmc
2026
置信度 0.87
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Hemophilia is an inherited bleeding disorder caused by mutations in the F8 or F9 gene, leading to a deficiency or dysfunction of coagulation factors VIII or IX. While current treatments, such as factor replacement, extended half-life factors, and gene therapy,…
europepmc
2026
置信度 0.87
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Introduction The rise of Klebsiella pneumoniae (KP) and carbapenem-resistant KP (CRKP) poses grave threats to public health and food safety, creating an urgent demand for rapid point-of-care testing (POCT). Traditional detection methods are limited by laborato…
europepmc
2026
置信度 0.87
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Key message Biolistic particle bombardment was used to deliver CRISPR-Cas9 ribonucleoprotein complexes (RNP) into the shoot apical meristem tissue of citrus and axillary meristem tissue of poplar, generating directed mutations in target genes. The use of meris…
europepmc
2026
置信度 0.87
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Crops increasingly face overlapping stresses such as heat, drought, salinity, and pathogens that conventional breeding or genome editing rarely overcome in combination. To address this, we propose CRISPR-enabled horizontal gene transfer (CRISPR-HGT) as a progr…
europepmc
2026
置信度 0.87
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Corneal neovascularization is a sight-threatening condition for which current treatments such as anti-VEGF agents are limited by invasiveness and side effects. We present the first non-viral, CRISPR/Cas9-based gene therapy delivered via topical eye drops that …
europepmc
2026
置信度 0.87
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Plant-associated microbiomes are central to crop productivity, nutrient efficiency, and stress resilience, yet conventional microbiome manipulation strategies, largely based on microbial inoculation and agronomic management, often suffer from inconsistent fiel…
europepmc
2026
置信度 0.94
biologysynthetic biologymicrobiologycomputational biologybiotechnology
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Fusarium wilt disease of lettuce caused by the soilborne fungus Fusarium oxysporum f. sp. lactucae (Fola) causes substantial crop losses worldwide. Four races of Fola have been identified with race 1 (Fola1) and the more recently emerged race 4 (Fola4) predomi…
europepmc
2026
置信度 0.87
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Global climate change poses significant challenges to agricultural production and food security, particularly in high-latitude regions such as northern China, where low temperatures, drought, and extreme weather severely affect crop growth and development. Add…
europepmc
2026
置信度 0.83
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Duck adenovirus 3 (DAdV-3) was firstly identified in Muscovy ducklings exhibiting slight pericardial effusion, swelling, and hemorrhaging in the kidneys, which is a poorly characterized duck virus. This study established a CRISPR/Cas12a coupled with a Lateral …
europepmc
2026
置信度 0.87
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Recent advances in gene therapy have highlighted the potential of CRISPR-based gene-editing systems combined with adeno-associated virus (AAV) vectors. However, the limited packaging capacity of AAV remains a significant challenge for the simultaneous expressi…
europepmc
2026
置信度 0.87
-
CRISPR-Cas9 has revolutionized the field of genome editing. While conventional gene supplementation therapies and the market of related gene therapy products are dominated by viral vectors, non-viral delivery strategies are increasingly being explored for in v…
europepmc
2026
置信度 0.87
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The European Food Safety Authority has issued several scientific opinions on plants, microorganisms and animals obtained through certain new genomic techniques (NGTs), following requests received by the European Commission. These scientific opinions provided c…
europepmc
2026
置信度 0.83
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Adoptive T cell therapies can deliver curative responses for refractory patients with B cell malignancies, yet clinical activity in solid tumors remains inconsistent. Tumor-intrinsic barriers dominating this inconsistency include the immunosuppressive solid tu…
europepmc
2026
置信度 0.87
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Main conclusion Trait development for commercial heirloom tomatoes can be advanced by optimization of tissue culture and transformation via Agrobacterium and CRISPR/Cas9 mutagenesis. Genetic improvement using new genome editing approaches relies on the efficie…
europepmc
2026
置信度 0.87
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Genetically modified (GM) plants have revolutionized agriculture for more than three decades. The production of a GM plants is a complex, multi-stage process. Several key methods are available for generating GM plants. The choice of transformation method depen…
europepmc
2026
置信度 0.87
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The emergence of CRISPR/Cas9 technology has transformed the landscape of gene editing, allowing for precise alterations in DNA that hold great promise for research and potential therapies. However, a significant concern is the occurrence of off-target effects,…
europepmc
2026
置信度 0.94
psychologycomputer scienceneurosciencedata sciencecomputational biology
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Rapeseed is a major oil crop worldwide, producing both oil and a high amount of protein. However, the use of its seed meal as a protein source for animal feed is limited by antinutritional factors, such as sinapine, which reduces nutrient absorption and affect…
europepmc
2026
置信度 0.87
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Antibiotic resistance (ABR) is a leading cause of death and a major public health threat globally. Without appropriate interventions, annual ABR-associated deaths have been projected to reach 10 million by 2050 worldwide. Hence, it is critical to develop novel…
europepmc
2026
置信度 0.87
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The environmental and therapeutic application of genetically engineered microorganisms necessitates the development of robust, irreversible biocontainment systems. In this study, we present an eEGM (editing-driven essential gene multiplex inactivation) module …
europepmc
2026
置信度 0.87
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CRISPR/Cas9-based gene editing of the malaria parasite Plasmodium falciparum has emerged as a transformative tool for advancing functional studies on parasite biology and identifying new therapeutic targets. Currently applied CRISPR/Cas9 methodologies depend o…
europepmc
2026
置信度 0.87
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Epilepsy affects millions of people globally and is marked by unpredictable seizures due to excessive brain activity. These seizures not only vary widely in brain origin and severity but can also be associated with a range of factors - from head injuries to in…
europepmc
2026
置信度 0.87
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Industrial activities and legacy contamination have generated metal-laden soils, radionuclide plumes, solvent-saturated sediments, and acidified pollutants. These are complex, hostile matrices where chemical treatments often redistribute rather than eliminate …
europepmc
2026
置信度 0.87
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Introduction The rapid advancement and adoption of CRISPR-Cas technologies in crop improvement has significantly outpaced existing regulatory frameworks, leading to inconsistencies in the global oversight of gene-edited organisms. As governments and internatio…
europepmc
2025
置信度 0.87
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The escalating global demand for animal products-meat, milk, and eggs-requires sustained advances in the efficiency, sustainability, and biological performance of livestock production systems [...].
europepmc
2026
置信度 0.81
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Antimicrobial resistance (AMR) is commonly framed as a consequence of mutation and selection, yet this perspective does not fully explain the speed and scale of global resistance dissemination. Here, we argue that AMR is better understood as an amplification p…
europepmc
2026
置信度 0.87
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CRISPR/Cas9 is a cornerstone of plant genome editing, yet the determinants of editing efficiency for a given single-guide RNAs (sgRNAs) and DNA double-strand break (DSB) repair outcomes remain poorly understood, particularly in plants. Here, we generated a lar…
europepmc
2026
置信度 0.87
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This essay analyses two novels that explore reproductive scenarios which may be implemented in the near future: human germline genome editing and in vitro gametogenesis (IVG), dramatised in Eve Smith's Off-Target (2022) and Kira Peikoff's Baby X (2024), respec…
europepmc
2026
置信度 0.87
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Virus-induced genome editing (VIGE) is expanding plant functional genomics by enabling precise and heritable genome modification across diverse species. While classical model systems such as Arabidopsis thaliana have provided foundational genetic insights, man…
europepmc
2026
置信度 0.87
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Key message The targeted mutation of the strigolactone biosynthetic gene, CCD8, through genome-editing CRISPR-Cas9 induces dwarfism and enhanced branching in Eustoma grandiflorum. In ornamental plants, modifying shoot architecture is a major breeding objective…
europepmc
2026
置信度 0.87
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Human induced pluripotent stem cells (iPSCs) are transforming adoptive cell therapy by combining unlimited self-renewal, broad differentiation potential, and high amenability to genome engineering. These attributes make iPSCs a versatile source for the develop…
europepmc
2026
置信度 0.87
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Key message CRISPR-Act3.0 is a robust tool for modulating fatty acid profiles in plants. We demonstrate that Agrobacterium rhizogenes-mediated transformation provides a rapid, cost-effective, and equipment-independent platform for validating sgRNA efficiency a…
europepmc
2026
置信度 0.87
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Mosquito-borne virus surveillance increasingly requires rapid, distributed detection of co-circulating pathogens, serotypes, and lineages across clinical and vector-sampling sites. CRISPR-Cas platforms offer a programmable toolkit for this purpose, but their r…
europepmc
2026
置信度 0.87
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Clustered regularly interspaced short palindromic repeats (CRISPR)-based genome and epigenome editing hold great promise for precision therapy, yet their clinical translation is limited by delivery challenges. Here, we present a transient, DNA-free virus-like …
europepmc
2026
置信度 0.87
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Antimicrobial resistance (AMR) is escalating worldwide, posing a serious threat to global public health by driving infections that are no longer treatable with conventional antibiotics. CRISPR-Cas technology offers a programmable and highly specific therapeuti…
europepmc
2026
置信度 0.87
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Background Neurodegenerative diseases are a major and growing global health burden. Their pathogenesis is complex, and effective therapies remain limited. Gene editing and stem cell-based strategies are reshaping the therapeutic landscape. However, the field h…
europepmc
2026
置信度 0.87
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Hypomorphic DCLRE1C variants impair T and B cell development, leading to combined immunodeficiency (CID) or leaky severe combined immunodeficiency (SCID). Current treatment options, such as allogeneic hematopoietic stem cell transplantation (aHSCT), are associ…
europepmc
2026
置信度 0.87
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CRISPR technologies are transforming cardiovascular therapy development by creating an increasingly seamless pipeline from potential target discovery to clinical translation. What began as a genome-editing tool has evolved into a versatile platform that enable…
europepmc
2026
置信度 0.87
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Staphylococcus pseudintermedius is a common commensal bacterium of dogs. It becomes an opportunistic pathogen when it evades the immune and exogenous antimicrobial control to cause pyoderma, otitis, and postoperative infections in dogs. The shift from coloniza…
europepmc
2026
置信度 0.87
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Introduction Antimicrobial resistance (AMR) is a major One Health concern driven by many factors including unregulated animal farming. This study aimed to perform a comprehensive phenotypic and genotypic characterization of Escherichia coli isolated from backy…
europepmc
2026
置信度 0.87
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Type 1 diabetes is a chronic autoimmune disease involving the specific destruction of pancreatic β-cells and consequent absolute insulin deficiency. Although exogenous insulin replacement aids in hyperglycemic management, it does not cure the disease and incre…
europepmc
2026
置信度 0.87
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The evolutionarily conserved RNA-binding protein Muscleblind can function as both a splicing regulator in the nucleus and a mRNA stabilizer in the cytosol. C. elegans mbl-1/ Muscleblind undergoes alternative splicing to generate long and short isoforms that co…
europepmc
2026
置信度 0.87
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Terminology (used consistently throughout): "sequence-guided therapeutics" refers to interventions whose target selection, triage, and monitoring are anchored to pathogen sequence features (genes/variants) rather than phenotype alone; "programmability" refers …
europepmc
2026
置信度 0.87
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Double-stranded DNA (dsDNA) serves as a fundamental repository of genetic information and plays a pivotal role in the diagnosis and therapeutic management of diseases. However, the inherent stability of the DNA double helix under physiological conditions prese…
europepmc
2026
置信度 0.87
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Advances in functional genomic technology, notably CRISPR using Cas9 or Cas12, now allow for large-scale double perturbation screens in which pairs of genes are inactivated, allowing for the experimental detection of genetic interactions (GIs). However, as it …
europepmc
2026
置信度 0.87
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Tissue-restricted gene expression serves as a key indicator of functional importance within specific organs. Despite advances in high-throughput sequencing and genome editing over the past decade, this principle remains the gold standard for identifying candid…
europepmc
2026
置信度 0.87
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Roots, Tubers, and Bananas (RTB), including banana and plantain, cassava, yam, sweetpotato, and potato crops, share several defining features that set them apart from cereals and legumes. They are essential for food and nutritional security for hundreds of mil…
europepmc
2026
置信度 0.87
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Anti-CRISPR (Acr) proteins are phage-encoded anti-defense factors that suppress CRISPR-Cas immunity in bacteria. AcrIIA8 was previously identified as an inhibitor of Streptococcus pyogenes Cas9 (SpyCas9) through functional assays of metagenomic libraries. Here…
europepmc
2026
置信度 0.87
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The utilization of bacteria for tumor treatment has a long-standing history. In recent years, with the continuous advancement of genetic engineering technologies, genetically engineered bacteria have attracted widespread attention in tumor-related research; ho…
europepmc
2026
置信度 0.87
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The CRISPR–Cas system has revolutionized molecular diagnostics and gene editing, yet its clinical translation is hindered by delivery barriers, off-target activity, immunogenicity, and manufacturing challenges. Compared with viral vectors and synthetic non-vir…
europepmc
2026
置信度 0.87
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Porcine reproductive and respiratory syndrome (PRRS) remains one of the most significant health and welfare challenges in global pig production. The disease is associated with substantial economic losses, impaired herd health, increased antimicrobial use, and …
europepmc
2026
置信度 0.87
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Mutation rates often rise under environmental stress, a process known as stress-induced mutagenesis. Among abiotic factors, heat stress is a potent driver that elevates mutation rates and enhances genetic variation, yet the underlying mechanisms in eukaryotes …
europepmc
2026
置信度 0.87
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The clustered regularly interspaced short palindromic repeat/associated protein 9 (CRISPR/Cas9) system has been used for the precise manipulation of target DNA, making efficient genome editing in cells a reality. The CRISPR/Cas9 system has shown great potentia…
europepmc
2026
置信度 0.87
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CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated nucleases) systems allow researchers to detect, capture, and even alter parts of an organism's genome. However, while the use of CRISPR-Cas has revolutionised many fields i…
europepmc
2026
置信度 0.87
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A major goal in CRISPR/Cas gene therapy delivery is achieving biocompatible, transient delivery of gene editing machinery directly in patients. Among emerging delivery options, extracellular vesicles (EVs) hold promise as a new class of non-viral biologically …
europepmc
2026
置信度 0.87
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Eye diseases caused by genetic mutations affect over 2.2 billion people worldwide. The development of CRISPR technology has opened exciting possibilities for how we diagnose and treat these conditions. However, designing effective CRISPR systems, managing pote…
europepmc
2026
置信度 0.87
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Background/Objectives : Immune checkpoints are critical regulatory pathways that maintain peripheral tolerance and prevent autoimmunity. Among these, the programmed death-1/programmed death-ligand 1 (PD-1/PD-L1) axis serves as a major inhibitory pathway that t…
europepmc
2026
置信度 0.87
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Protoplast-based systems provide a powerful and versatile platform for exploring how plants sense and respond to their environment. By enabling the direct delivery of proteins, DNA, and RNA into plant cells after cell wall removal, this approach facilitates pr…
europepmc
2026
置信度 0.87
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The advent of genetics, molecular biology, and genome sequencing has rapidly accelerated the development of elite genetic lines across various species, including poultry. It is now possible to introduce intra- or inter-species single nucleotide polymorphisms i…
europepmc
2025
置信度 0.87
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Alzheimer's disease is a devastating progressive neurodegenerative disorder characterized by extracellular amyloid-beta plaques and intracellular tau tangles. Despite recent advancements in amyloid-beta-targeting immunotherapies, achieving safe and definitive …
europepmc
2026
置信度 0.87
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Agricultural sustainability faces serious challenges from population growth, climate change and ecological degradation. Genetic modification (GM) technology can be regarded as a precise extension of the Green Revolution, aiming to balance yield enhancement wit…
europepmc
2026
置信度 0.87
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Conventional methods available for genome editing have proven non-specific, labour-intensive, and time-consuming. In this context, CRISPR/Cas technology represents a significant breakthrough. It is derived from a sophisticated microbial defence system consisti…
europepmc
2025
置信度 0.87
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Intervertebral disc degeneration (IDD) is the leading pathological cause of low back pain, while current clinical treatments are only palliative and cannot reverse the programmed cellular senescence driven by epigenetic dysregulation. This process is character…
europepmc
2026
置信度 0.87
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Marek's disease (MD), an important avian immunosuppressive and neoplastic diseases, has resulted in huge economic losses for the poultry industry worldwide. Over the past 50 years, the long-term prevalence of this disease and the growing immune pressure of MD …
europepmc
2026
置信度 0.87
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With an estimated number of 10 31 , bacteriophages are ubiquitous biological entities prevalent in various ecological niches (Hendrix et al., 1999;Mushegian, 2020). These viruses are natural predators of bacteria and pose a persistent threat to their populatio…
europepmc
2026
置信度 0.87
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The global rise of antimicrobial resistance (AMR) demands rapid and precise diagnostic tools capable of providing actionable results at the point of care. Traditional methods, including bacterial culture and PCR, face critical limitations: culture-based approa…
europepmc
2026
置信度 0.87
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Neurological and mental disorders are among the main causes of disability worldwide, affecting over three billion people and increasing the socioeconomic burden. Advances in molecular genetics and genome engineering have led to gene-targeted therapies that add…
europepmc
2026
置信度 0.87
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Functional validation of NLR genes is critical for confirming their specific roles and developing durable disease-resistant crops. The Fom-1 gene of Cucumis melo, controlling resistance to races 0 and 2 of F. oxysporum f. sp. melonis (FOM), had been identified…
europepmc
2026
置信度 0.87
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Rice serves as a fundamental staple crop, supporting the dietary needs of nearly one-third of the global population. This critical role necessitates immediate and strategic efforts to develop multi-attribute genotypes to ensure sustainable food and nutritional…
europepmc
2025
置信度 0.87
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Meristem function underlies organogenesis and yield potential in crop species, and its regulation depends on the crosstalk of genetic and hormonal networks that balance stem-cell niche maintenance and differentiation. During the shoot apical meristem (SAM) tra…
europepmc
2026
置信度 0.87
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Mutations in mitochondrial DNA can cause a wide range of neuromuscular and neurodegenerative diseases in humans. The heteroplasmy level, coexistence of both wild-type and mutant mtDNA within a cell, determines the manifestation and severity of disease symptoms…
europepmc
2026
置信度 0.87
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The emergence of advanced genome editing technologies has revolutionized research in life sciences, offering an unprecedented way to uncover unknown biological functions and innovative therapeutic strategies. Among all genome editing tools, CRISPR-Cas-based te…
europepmc
2026
置信度 0.87
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Sugarcane (Saccharum spp.) contributes approximately 80% of global sugar production and 40% of biofuel while serving as a promising feedstock for bioproducts (Brant et al., 2025). However, productivity faces mounting challenges from biotic threats including fu…
europepmc
2025
置信度 0.87
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Plant genome editing has undergone a transformative shift with the advent of advanced molecular tools, offering unprecedented levels of precision, flexibility and efficiency in modifying genetic material. While classical site-directed nucleases such as ZFNs, T…
europepmc
2025
置信度 0.87
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Lung adenocarcinoma is the most common form of lung cancer with a 5-year survival rate of 15%, largely due to asymptomatic metastasis and late diagnosis. Overexpression of Polycomb group (PcG) proteins, particularly EZH2, the catalytic component of Polycomb Re…
europepmc
2026
置信度 0.87
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The pursuit of sustainable livestock farming to meet the rising global protein demand has positioned myostatin (MSTN) gene editing as a key technology. However, the field's focus on the remarkable double-muscle phenotype has often overshadowed a systematic exa…
europepmc
2026
置信度 0.87
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Efficient and safe delivery of large genetic constructs such as CRISPR/Cas9 plasmids remains a critical bottleneck in gene therapy. In this work, the peptide-assisted lipofection (PAL) system was developed as a breakthrough non-viral vector for gene delivery, …
europepmc
2025
置信度 0.87
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Lactic acid bacteria (LAB) are pivotal microorganisms in the food industry. Current approaches for functional gene validation and trait improvement in LAB primarily rely on traditional gene editing and homologous recombination techniques. These methods are oft…
europepmc
2026
置信度 0.87
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CRISPR-based approaches can complement other genomics-based toxicology studies by enabling causal interrogation of gene function modulating chemical-induced toxicity. Moreover, CRISPR screens enable scalable and systematic identification of functional pathways…
europepmc
2026
置信度 0.87
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Myostatin ( MSTN ) gene knockout has attracted considerable interest for enhancing meat production in livestock species due to its well-established role as a negative regulator of skeletal muscle growth. In this study, we report the clinical outcome of a bi-al…
europepmc
2026
置信度 0.87
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We recently discovered distinct methylation patterns between the mitochondrial genome and the nuclear-encoded mitochondrial DNA sequences (NUMTs), with the mitochondrial genome being hypomethylated and NUMTs being hypermethylated. Given that genome editing usi…
europepmc
2026
置信度 0.87
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Cancer immunotherapy has recently become an essential approach for treating cancer, showing considerable promise as a substitute for surgery, radiation therapy, and conventional chemotherapy. It primarily aims to boost the host's natural defense system to comb…
europepmc
2026
置信度 0.87
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CRISPR/Cas12a technology, characterized by its distinctive trans-cleavage activity, has evolved beyond its gene-editing function to emerge as a powerful tool for molecular detection. This review systematically delineates its structural foundation and molecular…
europepmc
2026
置信度 0.94
computer sciencepharmacologymedicinetransformative learningbiological sciences not elsewhere classified
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Intervertebral disc degeneration (IVDD) is a leading cause of chronic low back pain, yet current clinical interventions remain largely palliative and fail to restore disc structure and function. While mesenchymal stem cells (MSCs), exosomes, and bioengineered …
europepmc
2026
置信度 0.87
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Biofilm mediated antimicrobial resistance (AMR) has become a critical global health and economic challenge, affecting both community and healthcare settings. Microbial Biofilms significantly enhance the antibiotic tolerance and cause the persistent and device-…
europepmc
2026
置信度 0.87
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Background/Objectives : Duchenne muscular dystrophy (DMD) is a fatal, progressive neuromuscular disorder caused by mutations in the dystrophin gene, leading to the absence of functional dystrophin protein. As the largest gene in the human genome, the DMD locus…
europepmc
2026
置信度 0.87
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Introduction The interaction between guide RNAs (gRNAs) and target DNA sequences is a critical factor in the effectiveness of CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated protein 9) gene editing. Predicting these int…
europepmc
2026
置信度 0.87
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CRISPR-Cas systems have transformed viral genetics by enabling precise and efficient manipulation of large DNA virus genomes. This review provides a practical framework for applying CRISPR technology to herpesviruses and other large DNA viruses as an alternati…
europepmc
2026
置信度 0.87